Saffron is one of the most important economic plants in the world that also has many pharmaceutical applications. Genus Crocus L. (Saffron) with about 90 species of the Iridaceae family in the northern hemisphere, especially in West Asia and Iran are scattered. The present study was to investigate the antimicrobial activity of extracts of various parts such as cover of corm, corm, perianth and stigma of the variety of saffron (C. sativus var. Haussknechtii) on some pathogenic microorganisms (Bacillus cereus, Enterococcus faecalis, Escherichia coli , Pseudomonas fluorescens, Salmonella typhimurium, Staphylococcus aureus) extrusion. Plant specimens were collected from four different habitats of Kermanshah province and then were identified in the herbarium of Razi University. After preparing raw plant extract four different parts of the plants (cover of corm, corm, perianth, stigma) by soaking (maceration) were extracted. Microbial tests using minimum inhibitory concentration (MIC) of the broth micro-dilution method were determined. The results of analysis by statistical tests showed a significant correlation (001/0