شيمي سبز براي ساخت نانوذرات MgO در ماتريس مزوپور تهيه شده از كاه برنج و مطالعه خواص اسپكتروسكوپي و ضد باكتري آن

ﭼﮑﯿﺪه: در ﺳﺎﺧﺖ و ﺷﻨﺎﺳﺎﯾﯽ ﻣﺎده ﻣﺰوﭘﻮر ﺑﺎ اﺳﺘﻔﺎده از ﺧﺎﮐﺴﺘﺮ ﮐﺎه ﺑﺮﻧﺞ ﺑﻪ ﻋﻨﻮان ﯾﮏ ﻣﻨﺒﻊ ﺳﯿﻠﯿﺲ ارزان و ﺟﺎﯾﮕﺰﯾﻦ ﮔﺰارش ﺷﺪه اﺳﺖ.ﮐﺎه ﺑﺮﻧﺞ ﺑﺮاي ﺗﻮﻟﯿﺪ ﺳﯿﻠﯿﺲ آﻣﻮرف در 500 درﺟﻪ ﺳﺎﻧﺘﯽ ﮔﺮاد ﺳﻮزاﻧﺪه ﺷﺪ و ﺳﭙﺲ ﺑﺮاي ﺗﻬﯿﻪ ﻣﺎده ﻣﺰوﭘﻮر ﻣﻮرد اﺳﺘﻔﺎده ﻗﺮار ﮔﺮﻓﺖ. ﻧﺎﻧﻮذرات ﻣﻨﯿﺰﯾﻢ اﮐﺴﯿﺪ ﺑﻄﻮر ﻣﻮﻓﻘﯿﺖ آﻣﯿﺰي ﺑﺎ روش واﮐﻨﺶ ﺣﺎﻟﺖ ﺟﺎﻣﺪ در ﻣﺎﺗﺮﯾﺲ ﻣﺰوﭘﻮر ﺳﻨﺘﺰ ﺷﺪﻧﺪ. ﻧﻤﻮﻧﻪ ﻫﺎي ﺳﻨﺘﺰ ﺷﺪه ﺑﺎ اﺳﺘﻔﺎده از SEM ،FTIR ،XRD و TEMﻣﻮرد ارزﯾﺎﺑﯽ و ﺷﻨﺎﺳﺎﯾﯽ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﻓﻌﺎﻟﯿﺖ ﺿﺪ ﻣﯿﮑﺮوﺑﯽ ﻧﺎﻧﻮذرات ﺑﻪ روش ﺗﻌﯿﯿﻦ ﺣﺪاﻗﻞ ﻏﻠﻈﺖ ﻣﻤﺎﻧﻌﺖ ﮐﻨﻨﺪه از رﺷﺪ و اﻧﺘﺸﺎر از دﯾﺴﮏ، در ﻣﻘﺎﯾﺴﻪ ﺑﺎ آﻧﺘﯽ ﺑﯿﻮﺗﯿﮏ ﻫﺎي اﺳﺘﺎﻧﺪارد، ﻋﻠﯿﻪ اﺳﺘﺎﻓﯿﻠﻮﮐﻮﮐﻮس اورﺋﻮس و اﺷﺮﺷﯿﺎﮐﻠﯽ ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﺮﻓﺖ. ﻧﺘﺎﯾﺞ XRD، ﭘﯿﮏ ﻫﺎي ﭘﺮاش ﺑﺮاي ﻫﺮ دو ﺗﺮﮐﯿﺐ را در ﻧﺎﻧﻮﮐﺎﻣﭙﻮزﯾﺖ ﻧﺸﺎن ﻣﯽدﻫﺪ. ﻣﻄﺎﻟﻌﺎت XRD ﻧﺸﺎن ﻣﯽدﻫﺪ ﮐﻪ ﻧﺎﻧﻮ ذرات MgO ﺳﻨﺘﺰ ﺷﺪه داراي ﺳﺎﺧﺘﺎر ﻣﮑﻌﺒﯽ ﻣﯽﺑﺎﺷﻨﺪ. . ﺗﺼﺎوﯾﺮ ﻣﯿﮑﺮوﺳﮑﻮپ اﻟﮑﺘﺮوﻧﯽ ﻋﺒﻮري، ذرات ﮐﻮﭼﮏ ﻣﺘﻌﻠﻖ ﺑﻪ ﻧﺎﻧﻮذرات ﻣﻨﯿﺰﯾﻢ اﮐﺴﯿﺪ را ﺑﺎ ﻗﻄﺮ ﺣﺪاﮐﺜﺮ 19 ﻧﺎﻧﻮﻣﺘﺮ ﻧﺸﺎن داد.

The fabrication and characterization of mesoporous material using rice straw ash as an alternative cheap silica source is reported. Rice straw, combusted at 500 ºC for the production of amorphous silica, has been used for the preparation of mesoporous material. Magnesium oxide nanoparticles were successfully synthesized in mesoporous matrix through a solid state reaction method. The synthesized samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), and Scanning electron microscope (SEM). Disk diffusion method and MIC were used to evaluate the antimicrobial activity of nanoparticles on Staphylococcus aureus and Escherichia coli compared to standard commercial antibiotic disks. XRD results reveal diffraction peaks for each of the two compounds in the nanocomposite. The XRD studies show that the synthesized MgO nanoparticles have cubic structure. Transmission electron microscopy images showed small particles belong to magnesium oxide nanoparticles with maximum diameter 19 nm. MgO nanoparticles showed the bactericidal activity against both Gram-positive and Gram-negative bacteria. MgO indicate strong antibacterial activity related to alkalinity and active oxygen species. The zone diameters of MgO/RSA-MCM-41 nanocomposite were 17 mm for Gram-negative (E. coli) and 15 mm for Gram-positive (S. aureus) bacteria.