ﺑﺮرﺳﯽ اﺛﺮ ﻧﺎﺧﺎﻟﺼﯽ ﻣﺲ ﺑﺮ ﻗﺎﺑﻠﯿﺖ آﻧﺘﯽ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ ﻧﺎﻧﻮذرات ZnSe آﻻﯾﯿﺪه ﺑﻪ ﻣﺲ ﻋﻠﯿﻪ ﺑﺎﮐﺘﺮيﻫﺎي ﮔﺮم ﻣﻨﻔﯽ اﺷﺮﯾﺸﯿﺎ ﮐﻮﻻي و ﺳﻮدوﻣﻮﻧﺎس آﺋﺮوژﯾﻨﻮزا

در ﭘﮋوﻫﺶ ﺣﺎﺿﺮ، اﺑﺘﺪا ﻧﺎﻧﻮذرات ZnSe:Cu ﺑﺎ درﺻﺪﻫﺎي ﻣﺨﺘﻠﻒ ﻧﺎﺧﺎﻟﺼﯽ ﻣﺲ (0، 0/1، 0/75 و 1/5 درﺻﺪ) و ﻫﻤﭽﻨﯿﻦ ZnSe ﺗﺤﺖ ﺗﺎﺑﺶ ﻣﺎﯾﮑﺮووﯾﻮ ﺑﻪ ﻣﺪت 6 دﻗﯿﻘﻪ ﺑﻪ روش آﺑﯽ ﺗﻬﯿﻪ ﺷﺪه و ﺳﭙﺲ، ﺧﻮاص آﻧﺘﯽ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ آنﻫﺎ روي دو ﺑﺎﮐﺘﺮي ﮔﺮم ﻣﻨﻔﯽ اﺷﺮﯾﺸﯿﺎ ﮐﻮﻻي و ﺳﻮدوﻣﻮﻧﺎس آﺋﺮوژﯾﻨﻮزا، ﺑﻪ روش اﻧﺘﺸﺎر دﯾﺴﮏ ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﺮﻓﺖ. در اﯾﻦ ﭘﮋوﻫﺶ ﺑﻪ ﻣﻨﻈﻮر ﺳﻨﺘﺰ ﻧﺎﻧﻮذرات ﻣﺬﮐﻮر، ﭘﻮدرﻫﺎي ﺳﻠﻨﯿﻮم و ﺳﺪﯾﻢ ﺑﻮروﻫﯿﺪرﯾﺪ ﺑﻪ ﻋﻨﻮان ﻣﻮاد اوﻟﯿﻪ ﺗﻬﯿﻪ ﻣﻨﺒﻊ ﯾﻮن ﺳﻠﻨﯿﻮم و اﺳﺘﺎت روي ﺑﻪ ﻋﻨﻮان ﻣﻨﺒﻊ ﯾﻮن روي ﻣﻮرد اﺳﺘﻔﺎده ﻗﺮار ﮔﺮﻓﺘﻪ اﺳﺖ. ﺑﺎ ﺗﻨﻈﯿﻢ pH ﻣﺤﻠﻮل روي ﻣﻘﺪار 11/2، از ﺗﯿﻮﮔﻠﯿﮑﻮﻟﯿﮏ اﺳﯿﺪ (TGA) ﺑﻪ ﻋﻨﻮان ﻋﺎﻣﻞ ﭘﻮﺷﺸﯽ ﺟﻬﺖ ﻣﻤﺎﻧﻌﺖ از ﮐﻠﻮﺧﻪاي ﺷﺪن ﻧﺎﻧﻮذرات ﺳﻨﺘﺰ ﺷﺪه اﺳﺘﻔﺎده ﺷﺪه اﺳﺖ. ﻧﺘﺎﯾﺞ ﻣﺸﺨﺼﻪﻧﮕﺎري ﭘﺮاش ﭘﺮﺗﻮ اﯾﮑﺲ و ﻃﯿﻒ ﺳﻨﺠﯽ ﻓﺮاﺑﻨﻔﺶ - ﻣﺮﺋﯽ ﻣﺤﻠﻮل ﺣﺎوي ﻧﺎﻧﻮذرات ﻧﺸﺎن دﻫﻨﺪه ﮔﺴﺘﺮهي 1/94-2/14 ﺑﺮاي اﻧﺪازه ﻧﺎﻧﻮذرات و ﮔﺎف ﺣﺪود eV 3.50-3.65 ﻣﯽﺑﺎﺷﺪ. ﺟﻬﺖ ﻣﻄﺎﻟﻌﻪ ﺧﺎﺻﯿﺖ آﻧﺘﯽ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ ﻧﻤﻮﻧﻪﻫﺎ، اﺑﺘﺪا ﺑﺎﮐﺘﺮيﻫﺎي اﺷﺮﯾﺸﯿﺎ ﮐﻮﻻي و ﺳﻮدوﻣﻮﻧﺎس آﺋﺮوژﯾﻨﻮزا ﺑﺮ ﻣﺤﯿﻂ ﮐﺸﺖ ﻣﻮﻟﺮ ﻫﯿﻨﺘﻮن آﮔﺎر ﺗﻠﻘﯿﺢ ﺷﺪ و ﺳﭙﺲ دﯾﺴﮏﻫﺎي ﺑﺎرﮔﺬاري ﺷﺪه ﺗﻮﺳﻂ ﻧﺎﻧﻮذرات ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﺑﺮ روي آنﻫﺎ ﻗﺮار ﮔﺮﻓﺖ. ﭘﺲ از ﮔﺬﺷﺖ 18 ﺳﺎﻋﺖ از ﻣﺎﻧﺪﮔﺎري ﻧﻤﻮﻧﻪﻫﺎي ﻧﻬﺎﯾﯽ در اﻧﮑﻮﺑﺎﺗﻮر، ﻗﻄﺮ ﻫﺎﻟﻪﻫﺎي ﻣﻤﺎﻧﻌﺖ از رﺷﺪ ﻫﺮ ﺑﺎﮐﺘﺮي (ﻣﯿﺰان ﺣﺴﺎﺳﯿﺖ آنﻫﺎ) اﻧﺪازهﮔﯿﺮي ﺷﺪ. ﯾﺎﻓﺘﻪﻫﺎي اﯾﻦ ﭘﮋوﻫﺶ ﺣﺎﮐﯽ از آن اﺳﺖ ﮐﻪ اﯾﻦ ﻧﺎﻧﻮذرات داراي ﺧﺎﺻﯿﺖ آﻧﺘﯽ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ ﻗﺎﺑﻞ ﺗﻮﺟﻬﯽ ﺑﺮ روي ﺑﺎﮐﺘﺮيﻫﺎي ﮔﺮم ﻣﻨﻔﯽ اﺷﺮﯾﺸﯿﺎ ﮐﻮﻻي و ﺳﻮدوﻣﻮﻧﺎس آﺋﺮوژﯾﻨﻮزا ﻣﯽﺑﺎﺷﻨﺪ و ﻗﺎﺑﻠﯿﺖ اﺳﺘﻔﺎده در ﺣﻮزه داروﻫﺎي آﻧﺘﯽ ﺑﺎﮐﺘﺮي را دارا ﻣﯽﺑﺎﺷﻨﺪ. ﻧﺘﺎﯾﺞ ﺣﺎﺻﻞ از اﯾﻦ آزﻣﺎﯾﺶ ﻧﺸﺎن داد ﮐﻪ ﺑﯿﺸﺘﺮﯾﻦ ﻓﻌﺎﻟﯿﺖ ﻧﺎﻧﻮذرات آﻻﯾﯿﺪه، ﻣﺮﺑﻮط ﺑﻪ درﺻﺪ ﻧﺎﺧﺎﻟﺼﯽ ﻣﺲ 1/5 روي ﺑﺎﮐﺘﺮي اﺷﺮﺷﯿﺎ ﮐﻮﻻي ﻣﯽﺑﺎﺷﺪ. ﻫﻤﭽﻨﯿﻦ ﻧﻤﻮﻧﻪ ﺑﺪون ﻧﺎﺧﺎﻟﺼﯽ ﺗﻬﯿﻪ ﺷﺪه ﺗﺤﺖ زﻣﺎن ﺗﺎﺑﺶ ﻣﺎﯾﮑﺮووﯾﻮ ﺑﻪ ﻣﺪت 6 دﻗﯿﻘﻪ، داراي ﺑﯿﺸﺘﺮﯾﻦ ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ ﺑﺮاي ﺑﺎﮐﺘﺮي ﺳﻮدوﻣﻮﻧﺎس آﺋﺮوژﯾﻨﻮزا ﺑﻮد.

In the present research, ZnSe:Cu nanoparticles with different Cu-dopant content and also under 6 min microwave irradiation were synthesized by aqueous method, and then their antibacterial properties on two Escherichia coli and Pseudomonas aeruginosa gram negative bacteria were investigated. In this work, to synthesize the mentioned nanoparticles, Se and NaBH4, as initial raw materials of Se ion source, and zinc acetate as Zn ion source have been used. By adjusting the pH at 11.2, TGA has been used as capping agent to prevent agglomeration of nanoparticles. Results of XRD and UV- Visible spectroscopy, show the range of 1.94-2.14 for particles size and 3.50-3.65 eV for energy gap. To study the antibacterial properties, Escherichia coli and Pseudomonas bacteria were used as strain tests. Results of this research imply on that these nanoparticles have considerable antibacterial activity against the mentioned gram-negative bacteria, and have the usage ability in the field of antibacterial drugs. The highest antibacterial activity is devoted to the 1.5% Cu-doped nanoparticles on Escherichia coli. Also the undoped sample prepared under 6 min microwave irradiation had the highest antibacterial activity against the Pseudomonas aeruginosa.