ﺑﺎرﮔﺬاري رﯾﺰ ﻣﻐﺬي ﻧﺎﻧﻮ ذره ﺳﻠﻨﯿﻢ در ﺳﺎﺧﺘﺎر ﺑﺎﮐﺘﺮيﻫﺎي ﻻﮐﺘﻮﺑﺎﺳﯿﻞ و ﺑﺮرﺳﯽ ﺗﻮان ﺿﺪ ﻣﯿﮑﺮوﺑﯽ آن ﺟﻬﺖ ﺳﺎﺧﺖ ﻣﮑﻤﻞ داروﯾﯽ ﺿﺪ اﺳﻬﺎل

Nano-Selenium Loading on Structure of Lactobacillus and Evaluating its Antimicrobial Potency to Make Anti-Diarrhea Drug-Supplement

اﺷﺮﺷﯿﺎﮐﻠﯽ ﻋﺎﻣﻞ اﺻﻠﯽ اﺳﻬﺎل ﺧﻮﻧﯽ اﺳﺖ. ﺗﺤﻘﯿﻖ ﺣﺎﺿﺮ در راﺳﺘﺎي ﭘﯿﺸﻨﻬﺎد داروي زﯾﺴﺖ ﻣﺤﯿﻄﯽ ﺿﺪ اﺳﻬﺎل، ﺑﻪ ﺑﺮرﺳﯽ اﺛﺮات ﻫﻢاﻓﺰاﯾﯽ و ﺿﺪ ﻣﯿﮑﺮوﺑﯽ ﻧﺎﻧﻮ ذرات ﺳﻠﻨﯿﻢ و ﻻﮐﺘﻮﺑﺎﺳﯿﻞﻫﺎ روي اﺷﺮﺷﯿﺎﮐﻠﯽ ﭘﺮداﺧﺘﻪ اﺳﺖ. ﺗﻮان ﺿﺪ ﻣﯿﮑﺮوﺑﯽ ﻧﻤﻮﻧﻪﻫﺎي آزﻣﺎﯾﺸﯽ ﺑﻪ روش اﻧﺘﺸﺎر از دﯾﺴﮏ و ﭼﺎﻫﮏ ﺑﻪ ﺻﻮرت اﻧﺪازهﮔﯿﺮي ﻫﺎﻟﻪ ﻋﺪم رﺷﺪ ارزﯾﺎﺑﯽ ﺷﺪ. ﻫﻤﭽﻨﯿﻦ ﮐﻤﺘﺮﯾﻦ ﻏﻠﻈﺖ ﻣﻬﺎر رﺷﺪ (MIC) و ﮐﻤﺘﺮﯾﻦ ﻏﻠﻈﺖ ﮐﺸﻨﺪﮔﯽ )(MBC) ﺑﻪ روش رﻗﺖﺳﺎزي اﻧﺠﺎم ﮔﺮدﯾﺪ. ﻧﺘﺎﯾﺞ ﺑﺪﺳﺖ آﻣﺪه ﻧﺸﺎن داد در ﻫﺮ دو روش ﭼﺎﻫﮏ و دﯾﺴﮏ ﺑﯿﺸﺘﺮﯾﻦ ﻗﻄﺮ ﻫﺎﻟﻪ ﻋﺪم رﺷﺪ ﺑﻪ ﺗﺮﺗﯿﺐ ﺑﺎ ﻣﯿﺎﻧﮕﯿﻦ 23/6 و 23/36 ﻣﯿﻠﯽﻣﺘﺮ ﻣﺘﻌﻠﻖ ﺑﻪ ﺗﯿﻤﺎر " ﻧﺎﻧﻮ ذرات ﺳﻠﻨﯿﻢ ﺑﺎرﮔﺬاري ﺷﺪه در ﻻﮐﺘﻮﺑﺎﺳﯿﻞﻫﺎ" روي ﮔﻮﻧﻪ اﺳﺘﺎﻧﺪارد اﺷﺮﺷﯿﺎﮐﻠﯽ ﺑﻮد. ﺗﻌﯿﯿﻦ MIC وMBC ﻧﯿﺰ ﺣﺎﮐﯽ از آن ﺑﻮد ﮐﻪ دو ﺗﯿﻤﺎر "ﻧﺎﻧﻮ ذرات ﺳﻠﻨﯿﻢ ﺑﺎرﮔﺬاري ﺷﺪه در ﻻﮐﺘﻮﺑﺎﺳﯿﻞﻫﺎ" و "ﻧﺎﻧﻮ ذرات ﺳﻠﻨﯿﻢ+ﻻﮐﺘﻮﺑﺎﺳﯿﻞ" ﺑﻪ ﺗﺮﺗﯿﺐ ﺑﺎ ﻣﯿﺎﻧﮕﯿﻦ ﮐﻞ 590/7 و 615/38 ﻣﯿﮑﺮوﮔﺮم ﺑﺮ ﻣﯿﻠﯽﻟﯿﺘﺮ ﺑﺮاي MIC و ﻣﯿﺎﻧﮕﯿﻦ ﮐﻞ 955/32 و 1195/67 ﻣﯿﮑﺮوﮔﺮم ﺑﺮ ﻣﯿﻠﯽﻟﯿﺘﺮ ﺑﺮاي MBC، ﭘﺎﯾﯿﻦﺗﺮﯾﻦ ﻣﻘﺎدﯾﺮ را ﻧﺴﺒﺖ ﺑﻪ ﺳﺎﯾﺮ ﺗﯿﻤﺎرﻫﺎ داﺷﺘﻨﺪ (0/05 < P). ﺗﻤﺎﻣﯽ آزﻣﻮنﻫﺎ ﺣﺎﮐﯽ از ﺗﻮان ﺿﺪ اﺳﻬﺎﻟﯽ ﺑﺎﻻي ﻧﻤﻮﻧﻪ "ﻧﺎﻧﻮ ذرات ﺳﻠﻨﯿﻢ ﺑﺎرﮔﺬاري ﺷﺪه در ﻻﮐﺘﻮﺑﺎﺳﯿﻞﻫﺎ" ﺑﻮد. از اﯾﻨﺮو ﺑﻪ ﺷﺮط ﺗﮑﺮار آزﻣﺎﯾﺶ در ﻣﻄﺎﻟﻌﺎت آﺗﯽ و اﻃﻤﯿﻨﺎن از ﺻﺤﺖ ﻧﺘﺎﯾﺞ ﺑﺪﺳﺖ آﻣﺪه، ﻣﯽﺗﻮان ﺳﺎﺧﺖ داروي زﯾﺴﺖ ﻣﺤﯿﻄﯽ ﺑﺎ اﯾﻦ ﻓﺮﻣﻮﻻﺳﯿﻮن را ﺟﻬﺖ اﺳﺘﻔﺎده درﻣﺎﻧﯽ ﺗﻮﺻﯿﻪ ﻧﻤﻮد.

Escherichia coli (E.coli) is the most important species that causes dysentery. Present study was conducted to investigate the synergistic antimicrobial effects of Nano-selenium and lactobacillus on E.coli to suggest anti-diarrhea bio-drug. The antimicrobial effects of experimental treatments against E.coli were evaluated by disk diffusion and well diffusion methods as a measure of the growth inhibition zone. Moreover, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by micro-dilution method. The results of the well and disk diffusion methods showed that in both tests, the highest growth inhibition zone of the "Nano selenium-loaded lactobacillus" treatment on the standard strain of E.coli was 23/6 and 23/36 mm respectively. The results of MIC and MBC determination showed that in all experimental periods, "Nano selenium-loaded lactobacillus" and "Nano-selenium + lactobacillus" treatments with 590/7 and 615/38 μg/ml for MIC and 955/32 and 1195/67 μg/ml for MBC had the lowest amounts compared to other treatments (P<0.05). The results of this experiment confirmed the anti-diarrhea ability of "Nano selenium-loaded lactobacillus" treatment. Therefore, provided that this test be repeated in future studies and ensured the accuracy of these results, manufacturing of bio-drug with this formulation may be advised for prevention or treatment of diarrheal disease.