RAPD در مزارع چغندرقند استان خراسان با استفاده از نشانگرهاي مولكولي Fusarium oxysproum F.SP.betae بررسي تنوع ژنتيكي گروه هاي سازگار قارچ

RAPD analysis of genetic diversity within and among vegetative compatibility groups (VCGs) of Fusarium oxysporum Esp. betae in sugar beet fields of Khorasan, Iran

Random amplified polymorphic DNA (RAPD) markers were used to assess the genetic diversity within and among VCGs of Fusarium oxysporum f. sp. betae in sugar beet fields of Khorasan, Iran. Nine pathogenic isolates from 9 different VCGs along with one pathogenic self-incompatible isolate were selected as representatives. RAPD reactions were performed on genomic DNA, extracted from mycelia] mass using University of British Columbia (UBC) primers. Six primers amplified 117 scorable RAPD loci, of which 83 loci (71%) were polymorphic. RAPD profiles could genitically distinguish different VCGs from each other. Cluster analysis placed the self-incompatible isolate GL9 as an outgroup. Estimation of genetic distance or genetic similarity between isolates of each VCGs revealed a higher level of genetic relatedness within a VCG compared to that of among VCGs. The non-pathogenic isolate GL3 was placed with pathogenic isolates KR2 and N36 in the same cluster. However, cluster analysis showed that the isolates KR2 and N36 had by far closer relationship with each other than with isolate GL3. Results of cluster analysis also confirmed the correlation between VCGs and geographic origins of isolates.