GIARDIA LAMBLIA جهت كشت RPMI 1640 استفاده از كشت

Invitro cultivation of Giardia lamblia by use of RPMI 1640

Protozoa of the genus Giardia lamblia lives in proximal part of the small intestine of humans and causes disturbance, disease and malabsorption, especially in children. Since this protozoa is one of the most prevalent pathogens in humans and is very important in the field of medicine, therefore, investigation about this parasite is necessary. Biological study of protozoa needs in vitro culture of trophozoites. Due to the development of serological tests and need for parasite antigens, in vitro cultivation of trophozoite is warranted. In this study, RPMI 1640 culture medium was used for Giardia growth and reproduction. Giardia cysts were purified by the four - layer sucrose method. After induction by acid solution, cycts were transferred to culture tubes and incubated in 37°C. After 24 hours, samples were checked once a day for one week. From 53 culture samples,Giardia lamblia trophozoite was seen in 25 samples. Reproduction of protozoa in a large scale was not possible for a long time. Mean period of trophozoite viability was found to be 38.6 hours. Results of this study demonstrated that RPMI 1640 can be used for Giardia excystation and maintenance, but it is necessary to add other nutritional materials for reproduction and passage of trophozoites.