پديد آورندگان :
حافظي، فرهاد دانشگاه علوم پزشكي ايران - بيمارستان حضرت فاطمه - بخش جراحي ترميمي و پلاستيك - گروه جراحي ترميمي و پلاستيك، , علمي راد، حميد دانشگاه علوم پزشكي ايران - بيمارستان حضرت فاطمه - بخش جراحي ترميمي و پلاستيك - گروه جراحي ترميمي و پلاستيك، , پدرام، ميرسپاس دانشگاه علوم پزشكي تهران
چكيده فارسي :
ﺯﻣﻴﻨﻪ ﻭ ﻫﺪﻑ: ﻣﻴﻮﺓ ﮐﻴﻮﻱ (Actinidia Deliciosa) ﺩﺭ ﺁﻣﺎﺩﻩ ﺳﺎﺯﻱ ﮔﻮﺷﺖ ﺟﻬﺖ ﻣﺼﺎﺭﻑ ﺁﺷﭙﺰﻱ ﺑﮑﺎﺭ ﻣﻲ ﺭﻭﺩ. ﺍﻳﻦ ﻣﺎﺩﻩ ﺑﻪ ﺳﺮﻋﺖ ﻭ ﺑﻄﻮﺭ ﻣﺆﺛﺮﻱ ﺑﺎﻋﺚ ﻧﺮﻡ ﺷﺪﻥ ﻭ ﺣﺘﻲ ﻟﻪ ﺷﺪﻥ ﮔﻮﺷﺖ ﻣﻲ ﮔﺮﺩﺩ. ﻣﺸﺎﻫﺪ ﺍﻳﻦ ﺍﺛﺮ ﻭ ﻋﺪﻡ ﻭﺟﻮﺩ ﺗﺤﻘﻴﻘﺎﺕ ﻭ ﻣﻄﺎﻟﺐ ﻣﻨﺘﺸﺮ ﺷﺪﻩ ﺩﺭ ﺍﻳﻦ ﻣﻮﺭﺩ ﻣﺎ ﺭﺍ ﺑﺮ ﺁﻥ ﺩﺍﺷﺖ ﺗﺎ ﺑﺎ ﺍﻧﺠﺎﻡ ﺍﻳﻦ ﺗﺤﻘﻴﻖ
ﺩﺭ ﻣﺤﻴﻂ ﺁﺯﻣﺎﻳﺸﮕﺎﻩ ﺑﺮ ﺭﻭﻱ ﺣﻴﻮﺍﻧﺎﺕ ﺍﺯ ﺍﺛﺮ ﺑﺨﺸﻲ ﺁﻥ ﺁﮔﺎﻫﻲ ﻳﺎﺑﻴﻢ.
ﻣﻮﺍﺩ ﻭ ﺭﻭﺵ ﻫﺎ: 35 ﻣﻮﺵ ﺁﺯﻣﺎﻳﺸﮕﺎﻫﻲ ﻧﺮ ﺩﺭ ﺳﻪ ﮔﺮﻭﻩ ﺁﺯﻣﺎﻳﺶ G1, ﺷﺎﻫﺪ G2 ﻭ ﺷﺎﻫﺪ G3 ﻣﻮﺭﺩ ﺑﺮﺭﺳﻲ ﻗﺮﺍﺭ ﮔﺮﻓﺘﻨﺪ. ﺗﺤﺖ ﺑﻴﻬﻮﺷﻲ ﻋﻤﻮﻣﻲ، ﺳﻮﺧﺘﮕﻲ ﻣﺤﺪﻭﺩ ﺩﺭﺟﻪ 3 ﺩﺭ ﻧﺎﺣﻴﻪ ﭘﺸﺖ ﻫﺮ ﺳﻪ ﮔﺮﻭﻩ ﺑﺼﻮﺭﺕ ﻳﮑﺴﺎﻥ ﺍﻳﺠﺎﺩ ﺷﺪ. ﮔﺮﻭﻩ ﺁﺯﻣﺎﻳﺶ ﺑﻪ ﻭﺳﻴﻠﺔ ﻋﺼﺎﺭﺓ ﮐﻴﻮﻱ ﻭ ﮔﺮﻭﻩ ﮐﻨﺘﺮﻝ ﺑﻪ ﻭﺳﻴﻠﻪ ﻣﺎﺩﻩ ﺑﺪﻭﻥ ﮐﻴﻮﻱ ﺭﻭﺯﺍﻧﻪ ﭘﺎﻧﺴﻤﺎﻥ ﮔﺮﺩﻳﺪ ﻭ ﺑﺼﻮﺭﺕ ﻫﻔﺘﮕﻲ ﺯﺧﻢ ﻫﺎ ﻣﻌﺎﻳﻨﻪ ﻭ ﺍﺯ ﺁﻧﻬﺎ ﻓﺘﻮﮔﺮﺍﻓﻲ ﺗﻬﻴﻪ ﺷﺪ. ﺩﺭ ﺭﻭﺯ ﺑﻴﺴﺘﻢ ﻣﻄﺎﻟﻌﻪ ﺑﻴﻮﭘﺴﻲ ﺍﺯ ﻟﺒﻪ ﺯﺧﻢ ﻫﺎﻱ ﮔﺮﻭﻩ ﻫﺎﻱ G1 ﻭ G2 ﺑﺮﺩﺍﺷﺘﻪ ﺷﺪﻩ ﻭ
ﺗﻤﺎﻡ ﻣﻮﺵ ﻫﺎﻱ ﺩﺭ ﮔﺮﻭﻩ ﻫﺎﻱ G1 ﻭ G2 ﺑﻪ ﺭﻭﺵ ﺍﺳﺘﺎﻧﺪﺍﺭﺩ ﻣﻌﺪﻭﻡ ﮔﺮﺩﻳﺪ. ﭘﻨﺞ ﻣﻮﺵ ﺩﺭ ﮔﺮﻭﻩ G3 ﺗﺎ ﭘﺎﻳﺎﻥ ﺟﺪﺍ ﺷﺪﻥ Scab ﻧﮕﻬﺪﺍﺭﻱ ﺷﺪﻧﺪ.
ﻳﺎﻓﺘﻪ ﻫﺎ: ﺩﺭ ﮔـﺮﻭﻩ ﺁﺯﻣـﺎﻳﺶ، ﺍﺳـﮑﺎﺭ ﺩﺭ ﺗﻤﺎﻣﻲ ﻣﻮﺵ ﻫﺎ ﺑﻪ ﻃﻮﺭ ﮐﺎﻣﻞ ﮐﻨﺪﻩ ﺷـﺪﻩ ﺑﻮﺩ، ﺣﺎﻝ ﺁﻧﮑﻪ ﺩﺭ ﮔـﺮﻭﻩ ﮐﻨﺘـﺮﻝ ﻓﻘـﻂ ﺩﻭ ﻋﺪﺩ ﺍﺯ ﻣـﻮﺵ ﻫﺎ ﻓﺎﻗـﺪ ﺍﺳﮑﺎﺭ ﺑﻮﺩﻧﺪ ﻭ ﺍﻳﻦ ﺍﺯ ﻟﺤـﺎﻅ ﺁﻣﺎﺭﻱ ﻣﻌﻨﻲ ﺩﺍﺭ ﺑﻮﺩﻩ ﺍﺳﺖ P <0.001)(. ﻣﻴﺎﻧﮕﻴـﻦ ﺳﻄﺢ ﻣﻘﻄـﻊ ﺯﺧـﻢ ﺩﺭ ﮔـﺮﻭﻩ ﺁﺯﻣﺎﻳﺶ mm2 212/3869±88/80938 ﻭ ﺩﺭ ﮔـﺮﻭﻩ ﮐﻨﺘـﺮﻝmm2 388/4749±140/6967 ﺑﻮﺩ ﮐﻪ ﺍﻳﻦ ﺗﻔﺎﻭﺕ ﺍﺯ ﻟﺤـﺎﻅ ﺁﻣﺎﺭﻱ ﻣﻌﻨﻲ ﺩﺍﺭ ﺑـﻮﺩ P<0.001)(. ﺑﻪ ﺍﻳﻦ ﺗﺮﺗﻴﺐ ﺳﻄﺢ ﻣﻘﻄـﻊ ﺯﺧـﻢ ﺩﺭ ﻫـﺮ ﺩﻭ ﮔﺮﻭﻩ ﻣﻮﺭﺩ ﺁﺯﻣﺎﻳﺶ ﺑﻄﻮﺭ ﻣﻌﻨـﻲ ﺩﺍﺭﻱ ﻧﺴﺒﺖ ﺑﻪ ﮔـﺮﻭﻩ ﮐﻨﺘﺮﻝ ﮐﻤﺘﺮ ﺑﻮﺩ. ﺩﺭ ﮔـﺮﻭﻩ G3 ﭘﻮﺳﺖ ﺯﺧﻢ ﻫﺎ ﺑﻴﻦ ﺭﻭﺯ ﻫﺎﻱ 30 ﺍﻟﻲ 42 ﺑﻄﻮﺭ ﺧﻮﺩﺑﺨـﻮﺩﻱ ﺍﺯ ﺑﺴﺘﺮ ﺯﺧﻢ ﻫﺎ ﺟـﺪﺍ ﺷﺪﻧﺪ،ﺣﺎﻝ ﺁﻧﮑﻪ ﺍﻳﻦ ﺍﺗﻔﺎﻕ ﺩﺭ ﮔﺮﻭﻩ ﺁﺯﻣﺎﻳﺶ ﻗﺒﻞ ﺭﻭﺯ ﺑﻴﺴﺘﻢ ﺍﻧﺠـﺎﻡ ﭘﺬﻳﺮﻓﺖ. ﺩﺭ ﺑﺮﺭﺳﻲ ﭘﺎﺗﻮﻟـﻮﮊﻳﮏ ﺗﻔـﺎﻭﺕ ﻣﻬﻤﻲ ﺩﺭ ﺑﻴﻦ ﺩﻭ ﮔﺮﻭﻩ ﻭﺟﻮﺩ ﻧﺪﺍﺷﺖ P < 0.05)(.
ﻧﺘﻴﺠﻪ ﮔﻴـﺮﻱ: ﺩﺑـﺮﻳﺪﻣﺎﻥ، ﺍﻳﺠـﺎﺩ ﺍﭘﻴﺘﻠﻴـﻮﻡ ﺟـﺪﻳﺪ ﻭ ﺍﻧﻘﺒﺎﺽ ﺯﺧـﻢ ﺩﺭ ﮔـﺮﻭﻩ ﺁﺯﻣﺎﻳﺶ ﻧﺴﺒﺖ ﺑﻪ ﮔـﺮﻭﻩ ﮐﻨﺘـﺮﻝ ﺳﺮﻳﻌﺘـﺮ ﻭ ﺑﻬﺘﺮ ﺑﻮﺩﻩ ﺍﺳﺖ ﺑﻪ ﺩﻧﺒﺎﻝ ﺩﺑﺮﻳﺪﻣﺎﻥ ﺁﻧﺰﻳﻤـﺎﺗﻴﮏ ﺳﺮﻳﻊ، ﺑﻬﺒﻮﺩ ﺯﺧـﻢ ﭘﻴﺸﺮﻓﺖ ﻣﻨﺎﺳﺒﻲ ﺩﺍﺷﺘﻪ ﺍﺳﺖ ﻭ ﻫﻴـﭻ ﺍﺛﺮﻱ ﺍﺯ ﺗﺨـﺮﻳﺐ ﺑﺎﻓﺖ ﺳﺎﻟﻢ ﻭﺟﻮﺩ ﻧﺪﺍﺷﺘﻪ ﺍﺳﺖ.
چكيده لاتين :
Introduction & Objective: Actinidia deliciosa (kiwi fruit) is used as a meat tenderizer. It acts rapidly
and efficiently to soften meat and even to crush it, if it is allowed to work for more than a few hours.
Observing this effect and considering the lack of studies addressing this subject in the literature, the authors
decided to investigate the use of this natural remedy in an animal model.
Materials & Methods: Thirty-five male rats were divided randomly into three groups. Under general
anesthesia, a limited standard 3rd degree burn was produced on the back of each rat. For the intervention
group (G1, 15 rats), the wounds were covered with fresh kiwi fruit; for control groups 2 and 3 (G2, 15 rats;
G3, 5 rats), the dressing was a neutral ointment (Emulsifier the 1220). Weekly wound observations were
documented for all groups. Groups 1 and 2 were sacrificed on the day 20th, and the group 3 was kept alive
until complete eschar separation. The wounds of groups 1 and 2 were excised in full thickness and underwent
to microscopic evaluation.
Results: On the day 20th, all eschars had become detached and fallen off in the intervention group (G1),
whereas in groups G2 and G3 the eschars were still firmly attached to the base of the wounds (except in two
rats of group G2). This finding was statistically significant (P <0.001). The average wound surface area in
group G1 was 212.3869±88.80938 mm2, whereas in the group G2 it was 388.4749±140.6967 mm2, Thus, the
wound surface area was significantly (P < 0.001) smaller in the intervention group. The eschars in the group
G3 were separated spontaneously between days 30th and 42nd, while in all the rats of the kiwi-treated group,
this phenomenon occurred before the day 20th. The pathologic study revealed no considerable differences
between groups G1 and G2 (P < 0.05).
Conclusions: Debridement, neo-epithelialization and scar contraction were faster in the kiwi-treated
group than in the untreated group. Following rapid enzymatic debridement, healing appeared to progress
normally, with no evidence of damage to the adjacent healthy tissue.
