اثر حفاظت كبدي عصاره چاي سبز (Camellia sinensis) در سميت كبدي ناشي از سم ديازينون در موش‌هاي ماده

Hepatoprotective effects of green tea (Camellia sinensis) on diazinon induced liver damage in female mice

سابقه و هدف: ديازينون سبب افزايش توليد راديكال­هاي آزاد و آسيب به بافت­هاي بدن مي­شود. هدف از اين مطالعه، بررسي اثر چاي سبز روي بافت و آنزيم­هاي كبد در موش هاي تيمار شده با ديازينون بود. روش بررسي: در اين مطالعه تجربي، 40 سر موش ماده نژاد NMRI به 8 گروه تقسيم شدند: گروه كنترل1 ماده­اي دريافت نكرد؛ گروه كنترل2 و كنترل3 به ترتيب روغن زيتون و سالين را به مدت 7 روز دريافت كردند؛ گروه شاهد1 تزريق IP سم ديازينون را به مقدار mg/kg60 به مدت 7 روز و گروه شاهد2 و شاهد3 عصاره چاي سبز را به ترتيب با دوز 200 و 300 mg/kg به مدت 28 روز دريافت كردند؛ گروه آزمايشي 1و2 تحت تيمار با عصاره چاي سبز و ديازينون قرار گرفتند، به طوري كه ابتدا به ترتيب دوزهاي ذكر شده چاي سبز را براي 28 روز و ديازينون را به مقدار mg/kg60 از روز 14 به مدت 7 روز متوالي دريافت كردند. 48 ساعت پس از آخرين تزريق بافت كبد جدا شد و بررسي­هاي هيستوپاتولوژيك و سطح فعاليت آنزيم هاي كبدي بررسي شد. داده­ها با آزمون ANOVA تحليل شدند. يافته­ ها: فعاليت آنزيم­هاي كبدي در گروه شاهد1 نسبت به گروه كنترل افزايش داشت كه پس از به كارگيري عصاره چاي سبز در گروه­هاي آزمايشي 1و2 كاهش نشان داد (05/0

Background: Diazinon increases the production of free radicals and hurts the body tissues. The aim of this study was to evaluate the effect of green tea on tissue and liver enzymes in diazinon-treated rats. Materials and methods: In this experimental study, 40 NMRI female mice were divided into 8 groups: control group1 did not receive substance; control group 2 and control group 3 received olive oil and saline, respectively, for 7 days; shamgroup 1 received IP injection of diazinon in dose of 60 mg/kg for 7 days; the sham group 2 and sham group3 received green tea extracts with 200 and 300 mg/kg for 28 days. Experimental group 1 and 2 were treated with 200 and 300 mg/kg green tea extract for 28 days, respectively. Diazinon was prescribed with dose of 60 mg/kg since 14- day for 7 consecutive days in the experimental groups. 48 hours after the last injection, liver tissue was studied for histopathologic examination. The level of liver enzymes activity was evaluated. Data were analyzed by ANOVA. Results: The activity of liver enzymes in the control group 1 increased compared to the control group, which decreased after application of green tea extract in experimental groups 1 and 2. The histopathological results showed that hepatocytes were destroyed in the control group 1, while tissue necrosis was not observed in experimental groups 1 and 2. Conclusion: It could be concluded that the consumption of Camellia sinensis normalizes liver enzyme levels and improves liver tissue changes due to diazinon.