ﺑﺮرﺳﯽ اﺛﺮ ﭘﻤﺎد ﺳﻮﺧﺘﮕﯽ ادﯾﺐ درم ﺑﺮ ﺗﺮﻣﯿﻢ زﺧﻢ ﺳﻮﺧﺘﮕﯽ درﺟﻪ دو ﻋﻤﻘﯽ در ﻣﻮش ﺻﺤﺮاﺋﯽ

Effect of Adib Derm Ointment on the Healing of Full - Thickness Second Degree Burn Wound in Rat

زﻣﯿﻨﻪ و ﻫﺪف: ﺳﻮﺧﺘﮕﯽﻫﺎ ﻣﻌﻤﻮﻻً ﺑﻪ ﻋﻨﻮان ﺷﺪﯾﺪﺗﺮﯾﻦ ﻧﻮع ﺗﺮوﻣﺎ در ﻧﻈﺮ ﮔﺮﻓﺘﻪ ﻣﯽﺷﻮﻧﺪ. ﮔﯿﺎﻫﺎن داروﯾﯽ در ﻣﻘﺎﯾﺴﻪ ﺑﻪ ﻣﻮاد ﻣﺼﻨﻮﻋﯽ ﺑﺎ اراﺋﻪ درﻣﺎن اوﻟﯿﻪ ارزان، اﯾﻤﻦ و ﻃﺒﯿﻌﯽ ﻣﯽﺗﻮاﻧﺪ ﯾﮏ ﻣﻨﺒﻊ ﻣﻔﯿﺪ و ﻣﺆﺛﺮ در درﻣﺎن ﺳﻮﺧﺘﮕﯽ ﺑﺎﺷﺪ. ﻫﺪف ﻣﺎ در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﺮرﺳﯽ اﺛﺮ ﭘﻤﺎد ﺳﻮﺧﺘﮕﯽ ادﯾﺐ درم ﺑﺮ ﺗﺮﻣﯿﻢ زﺧﻢ ﺳﻮﺧﺘﮕﯽ درﺟﻪ دو ﻋﻤﻘﯽ در ﻣﻮش ﺻﺤﺮاﺋﯽ ﻣﯽﺑﺎﺷﺪ. ﻣﻮاد و روش ﻫﺎ: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﺠﺮﺑﯽ ﮐﻪ در آزﻣﺎﯾﺸﮕﺎه ﺣﯿﻮاﻧﺎت ﻣﺮﮐﺰ آﻣﻮزﺷﯽ درﻣﺎﻧﯽ ﺣﻀﺮت ﻓﺎﻃﻤﻪ )س( ﺗﻬﺮان در ﺳﺎل 1394 اﻧﺠﺎم ﺷﺪ، در ﻗﺴﻤﺖ ﺧﻠﻒ 24 ﻣﻮش ﺻﺤﺮاﺋﯽ ﻧﺮ ﺑﺎﻟﻎ ﻧﮋاد Sprague-Dawley ﺑﻪ وزن ﺗﻘﺮﯾﺒﯽ 250-300 ﮔﺮم ﺳﻮﺧﺘﮕﯽ درﺟﻪ دو ﻋﻤﻘﯽ ﺑﻪ اﺑﻌﺎد 2×4 ﺳﺎﻧﺘﯿﻤﺘﺮ اﯾﺠﺎد ﮔﺮدﯾﺪ و ﺳﭙﺲ در ﻣﺤﻞ ﺳﻮﺧﺘﮕﯽ ﮔﺮوه ﯾﮏ از ﭘﻤﺎد ﺳﻮﺧﺘﮕﯽ ادﯾﺐ درم )ﺷﺮﮐﺖ داروﯾﯽ ادﯾﺐ اﮐﺴﯿﺮ اﯾﺮان( و در ﮔﺮوه دوم ﮔﺎز وازﻟﯿﻦ )ﮐﻨﺘﺮل( ﭘﺎﻧﺴﻤﺎن ﺑﻪ ﺻﻮرت روزاﻧﻪ ﺗﺎ ﺑﻬﺒﻮد ﮐﺎﻣﻞ اﺳﺘﻔﺎده ﺷﺪ و روﻧﺪ ﺑﻬﺒـﻮدي زﺧﻢ ﺑﺎ ﻋﮑﺲ ﺑﺮداري ﺑﻪ ﺻﻮرت ﻫﺮ 7 روز ﯾﮏ ﺑﺎر ﺑﺎ ImageJ, ver. 1.45, (NIH, Maryland, USA) ﭘﺎﯾﺶ ﺷﺪ. در روزﻫﺎي 14 و 28 ﺟﻬﺖ ﺑﺮرﺳﯽ ﻣﯿﺰان ﮐﻼژن و ﺳﻠـﻮل ﻫﺎي اﻟﺘﻬﺎﺑﯽ ﻧﻤﻮﻧﻪ از زﺧﻢ ﺳﻮﺧﺘﮕﯽ ﺑﺮاي ﭘﺎﺗﻮﻟﻮژي ﻓﺮﺳﺘـﺎده ﺷﺪ. دادهﻫﺎي ﺟﻤـﻊآوري ﺷﺪه ﺑﺎ اﺳﺘﻔﺎده از ﻧﺮم اﻓﺰار SPSS وﯾﺮاﯾﺶ 19 و آزﻣﻮنﻫﺎي ﺗﯽ ﻣﻘﺎﯾﺴﻪ ﻣﯿﺎﻧﮕﯿﻦﻫﺎي دو ﮔﺮوه ﻣﺴﺘﻘﻞ و آزﻣﻮن آﻧﺎﻟﯿﺰ وارﯾﺎﻧﺲ اﻧﺪازهﮔﯿﺮيﻫﺎي ﻣﮑﺮر ﺗﺠﺰﯾﻪ و ﺗﺤﻠﯿﻞ ﺷﺪﻧﺪ. ﯾﺎﻓﺘﻪ ﻫﺎ: ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﻣﯿﺎﻧﮕﯿﻦ وﺳﻌﺖ زﺧﻢ در روزﻫﺎي 7 ﺗﺎ 21 در ﮔﺮوه ﭘﻤﺎد ادﯾﺐ درم از 1/088 ± 7/783 ﺑﻪ 1/062 ± 1/519 و در ﮔـﺮوه ﮐﻨﺘـﺮل از 1/386 ± 7/620 ﺑﻪ 0/867 ± 1/210 ﮐﺎﻫﺶ ﭘﯿﺪا ﮐﺮده اﺳـﺖ، اﻣﺎ اﯾﻦ ﮐﺎﻫـﺶ وﺳﻌﺖ زﺧـﻢ ﺑﯿﻦ دو ﮔﺮوه اﺧﺘـﻼف ﻣﻌﻨـﺎداري ﻧﺪاﺷـﺖ. ﻣﯿﺎﻧﮕﯿـﻦ اﻣﺘﯿـﺎز ﭘﺎﺗﻮﻟـﻮژي در روز 28 در ﮔـﺮوه ﭘﻤـﺎد ادﯾﺐ درم ﺑﯿﺸﺘـﺮ ﺑﻮده و اﯾﻦ اﺧﺘﻼف ﻣﻌﻨﺎدار ﺑـﻮده اﺳﺖ )0/001 < P(. ﻧﺘﯿﺠﻪ ﮔﯿـﺮي: ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ ﻧﺘﺎﯾﺞ ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ، ﻫﺮ ﭼﻨﺪ اﺳﺘﻨﺒﺎط ﻣﯽﮔﺮدد ﮐﻪ اﯾﻦ ﭘﻤﺎد ﺳﻮﺧﺘﮕﯽ ﺗﺎ ﺣﺪودي ﺗﺄﺛﯿﺮ ﻣﺜﺒﺖ داﺷﺘﻪ، اﻣﺎ ﭘﯿﺸﻨﻬﺎد ﻣﯽﮔﺮدد در زﻣﯿﻨﻪ ﺗﺄﺛﯿﺮ اﯾﻦ ﭘﻤﺎد ﺗﺮﮐﯿﺒﯽ ﻣﻄﺎﻟﻌﺎت ﺑﺎ ﺣﺠﻢ ﻧﻤﻮﻧﻪ وﺳﯿﻊﺗﺮ، ﺑﺎ ﺗﻐﯿﯿﺮ در روش اﻧﺠﺎم ﭘﺎﻧﺴﻤﺎن و ﻧﯿﺰ در زﺧﻢﻫﺎي ﺳﻮﺧﺘﮕﯽ ﺳﻄﺤﯽ اﻧﺠﺎم ﮔﺮدد.

Introduction & Objective: Burns are usually considered as the most violent trauma. Wound healing is a complex process that is usually associated with various growth and biological factors. During the process of wound healing, the dressing used for restoration and repairing tissues of the dermis and epidermis of wound. Medicinal plants provide initial treatment compared to synthetic materials are inexpensive, safe and natural can be a source of useful and effective in the treatment of burns. The aim of this study was to evaluate the effects of Adib Derm on the healing of full- thickness second degree burn wound in Rat. Materials & Methods: In this experimental study which was performed in the animal lab of the Hazrat Fatemeh Hospital in Tehran, 24 adult male rats with an approximate weight of 250- 300 grams, with deep burns of 2 cm 4×2 dimensions were prepared and divided into 2 groups of 12. We used Adib derm (Adib exir pharmaceutical company - Iranian scholar) in first group and Vaseline gas in the second group (control) to dressing every day until healing is complete and the wound healing process by shooting as every 7 days with Image J, ver. 1.45, (NIH, Maryland, USA) was monitored. On 14 and 28 to determine the amount of collagen and inflammatory cells were sent for pathological sample from burn wounds. Results: The results showed that during days 7 to 21 in each group reduced wound size (P < 0.001) and the reduction of wound size had no significant difference between the two groups. Average rating of pathology at day 28 was higher in the Adib Derm ointment group and this difference was significant (P < 0.001). Conclusions: According to the results of the present study, the effect of this ointment on the recovery of burn wounds was not as much as we expected. Thus, it is suggested that further studies perform with a larger sample size and with the change in the method of dressing and superficial burns.