تأثير عصاره الكلي حنا روي فاكتورهاي خوني ماهي كپور معمولي (Cyprinus carpio)

The Effect of Ethanolic Extract of Lawsonia inermis on the Hematological Parameters in Cyprinus carpio

ﺑﺮاي اﻧﺠﺎم اﯾﻦ آزﻣﺎﯾﺶ 280 ﻗﻄﻌﻪ ﻣﺎﻫﯽ ﮐﭙﻮر ﻣﻌﻤﻮﻟﯽ )ﺑﺎ وزن ﻣﺘﻮﺳﻂ 22/30±1/21 ﮔﺮم( ﺗﺤﺖ ﭼﻬﺎر ﺗﯿﻤﺎر ﺷﺎﻫﺪ، ﮔﺮوه ﺣﻤﺎم داﺋﻢ ﻋﺼﺎره اﻟﮑﻠﯽ 0/01 درﺻﺪ، ﮔﺮوه ﺣﻤﺎم داﺋﻢ ﻋﺼﺎره اﻟﮑﯽ 0/1 درﺻﺪ، ﮔﺮوه ﺣﻤﺎم داﺋﻢ ﻋﺼﺎره اﻟﮑﻠﯽ 0/5 درﺻﺪ ﺑﻪ ﻣﺪت 60 ز ﻗﺮار ﮔﺮﻓﺘﻨﺪ. در اﻧﺘﻬﺎي دوره ﺑﺎﻻﺗﺮﯾﻦ ﻣﯿﺰان ﮔﻠﺒﻮلﻫﺎي ﺳﻔﯿﺪ ﻣﺮﺑﻮط ﺑﻪ ﺗﯿﻤﺎر 0/01 و ﺑﻪ دﻧﺒﺎل آن ﺗﯿﻤﺎر 0/1 ﺑﻮده و در ﺗﯿﻤﺎر ﺷﺎﻫﺪ ﻣﯿﺰان ﮔﻠﺒﻮلﻫﺎي ﺳﻔﯿﺪ ﮐﻤﺘﺮﯾﻦ ﻣﻘﺪار را در ﻣﯿﺎن ﺳﺎﯾﺮ ﺗﯿﻤﺎرﻫﺎ ﻧﺸﺎن داد 0/05< p. ﺑﯿﺸﺘﺮﯾﻦ ﺗﻌﺪاد ﮔﻠﺒﻮلﻫﺎي ﻗﺮﻣﺰ ﻣﺮﺑﻮط ﺑﻪ ﺗﯿﻤﺎر 0/5 ﺑﺪون اﺧﺘﻼف ﻣﻌﻨﯽ دار ﺑﺎ ﺗﯿﻤﺎر 0/1 و ﺷﺎﻫﺪ ﺑﻮد0/05> p. ﻣﯿﺰان ﻫﻤﺎﺗﻮﮐﺮﯾﺖ در ﺷﺎﻫﺪ ﻣﻘﺎدﯾﺮ ﺑﺎﻻﺗﺮي را در ﻣﻘﺎﺑﻞ ﺗﯿﻤﺎرﻫﺎي 0/01، 0/1 و 0/5 ﻧﺸﺎن داد 0/05< p. در ﻣﻮرد ﻫﻤﻮﮔﻠﻮﺑﯿﻦ ﺗﯿﻤﺎر ﺷﺎﻫﺪ ﺑﺎﻻﺗﺮﯾﻦ ﻣﯿﺰان را دارا ﺑﻮد و ﺑﻪ دﻧﺒﺎل آن ﺗﯿﻤﺎر 0/01 ﻗﺮار داﺷﺖ0/05< p. در ﻣﻮرد ﺷﺎﺧﺺ ﻣﯿﺰان ﻣﺘﻮﺳﻂ ﻫﻤﻮﮔﻠﻮﺑﯿﻦ ﮔﻠﺒﻮﻟﯽ ﺑﺎﻻﺗﺮﯾﻦ ﻣﯿﺰان ﻣﺮﺑﻮط ﺑﻪ ﺗﯿﻤﺎر 0/01 و ﮐﻤﺘﺮﯾﻦ ﻣﻘﺪار ﻣﺮﺑﻮط ﺑﻪ ﺗﯿﻤﺎر 0/5 ﺑﻮد0/05< p. ﺗﯿﻤﺎر ﺷﺎﻫﺪ از ﻧﻈﺮ ﻋﺪدي در رﺗﺒﻪ دوم ﻗﺮار داﺷﺖ. ﻋﺼﺎره ﺣﻨﺎ در ﭘﺎﯾﺎن دوره 28 ﻏﻠﻈﺖ ﺳﺒﺐ ﺑﺎﻻ رﻓﺘﻦ ﻣﺘﻮﺳﻂ ﻫﻤﻮﮔﻠﻮﺑﯿﻦ ﮔﻠﺒﻮﻟﯽ در ﺗﯿﻤﺎرﻫﺎ ﺷﺪ و ﺣﺠﻢ ﻣﺘﻮﺳﻂ ﮔﻠﺒﻮﻟﯽ ﮐﻤﺘﺮﯾﻦ ﻣﻘﺪار را در ﺗﯿﻤﺎر 0/5 ﺑﯿﺸﺘﺮﯾﻦ ﻣﻘﺪار ﻣﺮﺑﻮط ﺑﻪ ﺗﯿﻤﺎر ﺷﺎﻫﺪ داﺷﺖ0/05< p

In this study, a total number of 280 Cyprinus carpio weighing on average (22.30±1.21g) were randomly distributed in four treatment groups, including control and three experimental groups. In experimental groups, fish were kept under constant bath of ethanolic extract of Lawsonia inermis (henna) at following rate of 0.01%, 0.1% and 0.5% for 60 days. At the end of experiment, the highest values of WBC were recorded in the 0.01% and 0.1% C.B of ethanolic extract of henna respectively and the lowest value were recorded in the control group (P< 0.05). The highest value of RBC were recorded in the 0.5% C.B. of ethanolic extract of henna (P>0.05). The highest value of hematocrite were recorded in the control group and the highest values of hemoglobin in the control group and in the 0.01% C.B. of ethanolic extract of henna, respectively. The highest value of mean corpuscular hemoglobin were recorded in the 0.01% C.B. of ethanolic extract of henna and the lowest value of mean corpuscular hemoglobin in the 0.5% C.B. of ethanolic extract of henna. Mean corpuscular hemoglobin increased under ethanolic extract of henna. The lowest value of mean corpuscular volume were recorded in the 0.5% C.B. of ethanolic extract of henna and the highest value of mean corpuscular volume were recorded in the control group.