پديد آورندگان :
وطن دوست حسين دانشگاه محقق اردبيلي - گروه زراعت و اصلاح نباتات , سيد شريفي رئوف دانشگاه محقق اردبيلي - گروه زراعت و اصلاح نباتات , خيري زاده اروق يونس دانشگاه محقق اردبيلي - گروه زراعت و اصلاح نباتات
كليدواژه :
اسيد چرب , عملكرد كوانتومي , محتواي كلروفيل , كلزا و هدايت روزنه‌اي
چكيده فارسي :
ﻣﻨﻈﻮر ﺑﺮرﺳﯽ اﺛﺮ ﺳﻄﻮح آﺑﯿﺎري و ﺑﺎﮐﺘﺮيﻫﺎي ﻣﺤﺮك رﺷﺪ ﺑﺮ ﻋﻤﻠﮑﺮد و ﺑﺮﺧﯽ ﺷﺎﺧﺺﻫﺎي ﻓﯿﺰﯾﻮﻟـﻮژ ي و ﺑﯿﻮﺷـ ﯿﻤﯿﺎﯾﯽ ﮐﻠـﺰا، آزﻣﺎ ﯾﺸـ ﯽ ﺑﻪﺻﻮرت ﻓﺎﮐﺘﻮرﯾﻞ در ﻗﺎﻟﺐ ﻃﺮح ﭘﺎﯾﻪ ﺑﻠﻮكﻫﺎي ﮐﺎﻣﻞ ﺗﺼﺎدﻓﯽ در اﯾﺴﺘﮕﺎه ﺗﺤﻘﯿﻘﺎت ﮐﺸﺎورزي اردﺑﯿﻞ ﺑﺎ ﺳﻪ ﺗﮑﺮار در ﺳﺎل زراﻋـ ﯽ 1395 اﺟﺮا ﺷﺪ. ﻋﻮاﻣﻞ ﻣﻮرد ﺑﺮرﺳﯽ ﺷﺎﻣﻞ ﺳﻄﻮح آﺑﯿﺎري در ﺳﻪ ﺳﻄﺢ )ﻗﻄﻊ آﺑﯿﺎري در 50 درﺻﺪ ﻣﺮﺣﻠﻪ ﮔﻞدﻫﯽ و ﺗﺸﮑﯿﻞ ﺧﻮرﺟﯿﻦ، ﻗﻄﻊ آﺑﯿﺎري در 50 درﺻﺪ ﻣﺮﺣﻠﻪ ﺗﺸﮑﯿﻞ داﻧﻪ، آﺑﯿﺎري ﮐﺎﻣﻞ ﺑﻪﻋﻨﻮان ﺷﺎﻫﺪ( و ﮐﺎرﺑﺮد ﮐﻮدﻫﺎي زﯾﺴﺘﯽ در ﭼﻬﺎر ﺳﻄﺢ )ﻋﺪم ﺗﻠﻘﯿﺢ ﺑﺬر ﺑﺎ ﺑﺎﮐﺘﺮي، ﺗﻠﻘﯿﺢ ﺑﺎ ﻧﯿﺘﺮوﺑﺎﮐﺘﺮ )Nitrobacter(، ﺳﻮدوﻣﻮﻧﺎس )Psedomonas( و آزوﺳﭙﺮﯾﻠﯿﻮم )Azospirillum( ﺑﻮدﻧﺪ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﻣﺤﺪودﯾﺖ آﺑﯽ، ﻋﻤﻠﮑﺮد، ﻋﻤﻠﮑﺮد ﮐﻮاﻧﺘﻮﻣﯽ، ﻫﺪاﯾﺖ روزﻧﻪاي، ﻣﺤﺘﻮاي ﮐﻠﺮوﻓﯿﻞ و ﻣﻘﺎدﯾﺮ ﻟﯿﻨﻮﻟﺌﯿﮏ، اوﻟﺌﯿﮏ، اﯾﮑﻮزﻧﻮﺋﯿﮏ و ﭘﺎﻟﻤﯿﺘﻮﻟﺌﯿﮏ اﺳـ ﯿﺪ را ﮐـﺎﻫﺶ و ﻣﺤﺘـﻮ اي اروﺳﯿﮏ اﺳﯿﺪ، آراﺷﯿﺪﯾﮏ اﺳﯿﺪ و ﭘﺮوﻟﯿﻦ را اﻓﺰاﯾﺶ داد. ﻣﻘﺎﯾﺴﻪ ﻣﯿﺎﻧﮕﯿﻦﻫﺎ ﻧﺸﺎن داد ﺑﺎﻻﺗﺮﯾﻦ ﻋﻤﻠﮑﺮد داﻧﻪ )1/62 ﺗﻦ در ﻫﮑﺘـﺎر(، ﻋﻤﻠﮑـﺮد ﮐﻮاﻧﺘﻮﻣﯽ )0/523(، ﻫﺪاﯾﺖ روزﻧﻪاي )14/7 ﻣﯿﻠﯽﻣﻮل ﺑﺮ ﻣﺘﺮﻣﺮﺑﻊ ﺑﺮﺛﺎﻧﯿﻪ(، ﻣﺤﺘﻮاي ﮐﻠﺮوﻓﯿﻞ )32/9(، اوﻟﺌﯿﮏ اﺳﯿﺪ )69/24 درﺻﺪ(، ﻟﯿﻨﻮﻟﺌﯿﮏ اﺳﯿﺪ )10/45 درﺻﺪ(، اﯾﮑﻮازاﻧﻮاﺋﯿﮏ اﺳﯿﺪ )1/34 درﺻﺪ( در ﺣﺎﻟﺖ ﺗﻠﻘﯿﺢ ﺑﺎ آزوﺳﭙﺮﯾﻠﯿﻮم و آﺑﯿﺎري ﮐﺎﻣﻞ و ﺑﯿﺸﺘﺮﯾﻦ ﻣﺤﺘﻮاي اروﺳﯿﮏ اﺳـ ﯿﺪ )2/89 درﺻﺪ( و آراﺷﯿﺪﯾﮏ اﺳﯿﺪ )0/94 درﺻﺪ( در ﻋﺪم ﺗﻠﻘﯿﺢ و ﻗﻄﻊ آﺑﯿﺎري در ﻣﺮﺣﻠﻪ ﺗﺸﮑﯿﻞ داﻧﻪ ﻣﺸﺎﻫﺪه ﺷﺪ. ﺑﯿﺸﺘﺮﯾﻦ ﻣﺤﺘﻮاي ﭘـﺮوﻟ ﯿﻦ )1/52 ﻣﯿﮑﺮوﮔﺮم در ﮔﺮم وزن ﺗﺮ( در ﺣﺎﻟﺖ ﺗﻠﻘﯿﺢ ﺑﺎ آزوﺳﭙﺮﯾﻠﯿﻮم و ﻗﻄﻊ آﺑﯿﺎري در ﻣﺮﺣﻠﻪ ﮔﻞدﻫﯽ و ﺗﺸﮑﯿﻞ ﺧﻮرﺟﯿﻦ ﺑﻪدﺳﺖ آﻣﺪ. ﺑﻪ ﻧﻈـﺮ ﻣﯽرﺳﺪ ﮐﺎرﺑﺮد ﺑﺎﮐﺘﺮيﻫﺎي ﻣﺤﺮك رﺷﺪ ﺑﻪدﻟﯿﻞ ﺑﻬﺒﻮد ﺑﺮﺧﯽ ﺷﺎﺧﺺﻫﺎي ﻓﯿﺰﯾﻮﻟﻮژﯾﮏ ﻣﺎﻧﻨﺪ ﻋﻤﻠﮑﺮد ﮐﻮاﻧﺘﻮﻣﯽ، ﻣﺤﺘﻮاي ﮐﻠﺮوﻓﯿـ ﻞ، ﻫـﺪ اﯾﺖ روزﻧﻪاي و ﭘﺮوﻟﯿﻦ ﻣﯽﺗﻮاﻧﺪ ﺑﺮاي ﺑﻬﺒﻮد ﺗﻮﻟﯿﺪ ﮐﻠﺰا ﺗﺤﺖ ﺷﺮاﯾﻂ ﮐﻢآﺑﯽ ﺗﻮﺻﯿﻪ ﺷﻮد.
چكيده لاتين :
In order to study the effect of irrigation levels and plant growth promoting rhizobacteria on yield, some physiological
and biochemical indices of rapeseed, a factorial experiment was conducted based on randomized complete block design
with three replications in Agricultural Research Station of Ardabil, Ardabil, Iran in 2016. The factors included in the
experiment were irrigation in three levels (irrigation withholding at 50% of flowering and pod formation stages,
irrigation withholding at 50% of grain formation stage, full irrigation or control) and application of bio-fertilizers in
four levels (no inoculation, inoculations with Nitrobacter, Psedomonas and Azospirillum). Results showed that water
limitation decreased yield, quantum yield, stomatal conductance, chlorophyll index, linoleic acid, eicosenoic acid and
palmitoleic acid, but increased erucic acid, arashidic acid and proline contents. Mean comparisons showed that the
highest grain yield (1.62 ton ha-1), quantum yield (0.523), stomatal conductance (14.7 mmol m-2 s-1), chlorophyll
content (32.9), linoleic acid (10.45%) and eicosenoic acid contents (1.34%) were obtained in inoculation with
Azosprillum and full irrigation. The highest contents of erucic acid (2.89%) and arashidic acid (0.94%) were observed at
no inoculation and irrigation withholding in grain formation stage. The maximum of proline content (1.52 μg g-1 FW)
was obtained in inoculation with Azosprillum and irrigation withholding at flowering and pod formation stage. It seems
that application of plant growth promoting rhizobacteria leads to improving of some physiological traits such as
quantum yield, chlorophyll content, stomatal conductance and proline content and can be recommended for profitable
canola production under water limitation conditions.
