ﺗﺄﺛﯿﺮ ﭘﯿﺶ آﻣﺎده ﺳﺎزي ﺑﺎ ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ ﺑﺮﻣﯿﺰان ﮐﻠﻮﺗﻮ و TNF-α ﻣﻮشﻫﺎي ﻣﺒﺘﻼ ﺑﻪ ﺑﯿﻤﺎري ام اس

The Effect of High Intensity Interval Training Preconditioning on Klotho and TNF-α Female Mice with Multiple Sclerosis

ﺳﺎﺑﻘﻪ و ﻫﺪف: ﻓﻌﺎﻟﯿﺖ ورزﺷﯽ ﻧﻘﺶ ﻣﻬﻤﯽ در اﻓﺰاﯾﺶ ﺑﯿﻮﻣﺎرﮐﺮﻫﺎي ﻣﯿﻠﯿﻦ ﺳﺎز دارد. ﻫﻤﭽﻨﯿﻦ، ﻋﻼﺋﻢ ﭘﺎﺗﻮﻟﻮژﯾﮏ را در ﻣﻮشﻫﺎي ﻣﺒﺘﻼ ﺑﻪ ام اس در ﻣﺪل آﻧﺴﻔﺎﻟﻮﻣﯿﻠﯿﺖ ﺧﻮد اﯾﻤﻨﯽ )EAE( ﺑﻬﺒﻮد ﻣﯽﺑﺨﺸﺪ. ﻫﺪف از ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ ارزﯾﺎﺑﯽ اﺛﺮ 6 ﻫﻔﺘﻪ ﭘﯿﺶ آﻣﺎده ﺳﺎزي ﺑﺎ ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ (HIIT) ﺑﺮ ﻣﯿﺰان ﮐﻠﻮﺗﻮ و ﭘﺮوﺗﺌﯿﻦ ﭘﺮوﺗﺌﻮﻟﯿﭙﯿﺪ )PLP( و ﻣﯿﺰان ﭘﯿﺸﺮﻓﺖ ﺑﯿﻤﺎري در ﻣﻮشﻫﺎي ﻣﺒﺘﻼ ﺑﻪ ﺑﯿﻤﺎري ام اس ﺑﻮد. ﻣﻮاد و روﺷﻬﺎ: ﺣﯿﻮاﻧﺎت ﭘﺲ از آﺷﻨﺎﺳﺎزي ﺑﺎ آزﻣﺎﯾﺸﮕﺎه ﺑﻪ دو ﮔﺮوه ﺗﻤﺮﯾﻦ (EX=30)و ﮐﻨﺘﺮل (Con=20 ) ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﭘﺲ از 4 ﻫﻔﺘﻪ، ﮔﺮوه ﺗﻤﺮﯾﻦ ﺑﻪ ﺳﻪ ﮔﺮوه و ﮐﻨﺘﺮل ﺑﻪ دو ﮔﺮوه ﺗﻘﺴﯿﻢ و EAEﺑﻪ ﻧﯿﻤﯽ از ﺣﯿﻮاﻧﺎت در ﮔﺮوه ﮐﻨﺘﺮل و دو ﺗﻤﺮﯾﻦ اﻟﻘﺎ ﺷﺪ. در ﮔﺮوه EX-EAE1ﺑﻌﺪ از 4 ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ، EAE اﻟﻘﺎ و ﭘﺮوﺗﮑﻞ ﺗﻤﺮﯾﻦ ﺗﺎ ﻫﻔﺘﻪ ﺷﺸﻢ اداﻣﻪ داﺷﺖ. در ﮔﺮوه EX-EAE2ﺑﻌﺪ از 4 ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ، EAE اﻟﻘﺎ و ﺗﻤﺮﯾﻦ ﻣﺘﻮﻗﻒ ﺷﺪ. ﮔﺮوه 6EX ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ را اﺟﺮا ﮐﺮدﻧﺪ. ﺑﺮاي ﺗﺤﻠﯿﻞ¬ﻫﺎي آﻣﺎري از آزﻣﻮن ﺗﺤﻠﯿﻞ وارﯾﺎﻧﺲ ﯾﮏ ﻃﺮﻓﻪ اﺳﺘﻔﺎده ﺷﺪ. ﯾﺎﻓﺘﻪ ﻫﺎ: ﻣﯿﺰان ﮐﻠﻮﺗﻮ و PLP در ﮔﺮوه EAE-EX1 در ﻣﻘﺎﯾﺴﻪ ﺑﺎ EAEو 0.0001≤EAE-EX2 (P و ﻫﻤﭽﻨﯿﻦ در ﮔﺮوه EX در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﮔﺮوه ﮐﻨﺘﺮل (0.01≤P≤0.001, P). اﻓﺰاﯾﺶ ﯾﺎﻓﺖ. ﻫﻤﭽﻨﯿﻦ، دﻣﯿﻠﯿﻨﻪ ﺷﺪن درﮔﺮوه EAE-EX1 درﻣﻘﺎﯾﺴﻪ ﺑﺎ ﮔﺮوه EAE ﮐﺎﻫﺶ ﯾﺎﻓﺖ (0.007≤P). در ﻫﺮ دو ﮔﺮوه ورزش ﻣﯿﺰان TNF-αﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه EAE ﮐﺎﻫﺶ داﺷﺖ (0.0001≤P). ﻧﺘﯿﺠﻪﮔﯿﺮي: ﻧﺘﺎﯾﺞ ﺗﺤﻘﯿﻖ ﺣﺎﺿﺮ ﻧﺸﺎن داد ﭘﯿﺶ آﻣﺎده ﺳﺎزي ﺑﺎ ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ ﻣﯿﺰان ﮐﻠﻮﺗﻮ و PLP را اﻓﺰاﯾﺶ و ﭘﯿﺸﺮﻓﺖ ﺑﯿﻤﺎري را در ﻣﺪل (EAE) ﮐﺎﻫﺶ داد.

Background Purpose Exercise activity plays an important role in increasing biomarkers such as Klotho and PLP and improves clinical outcome in mice with MS. In the present study, we evaluated whether 6 weeks of high-intensity interval training (HIIT) increases Klotho or PLP levels and decreased TNF-α in cerebellum tissue. Methodology: Animals were divided into two groups of training (EX) and control (Con). After 4 weeks, the exercise and control groups were divided into two groups and EAE was induced in half of the animals in the control and EX groups. In the EX-6 weeks group after 4 weeks of training, EAE induction and training protocol continued until the sixth week. In the EX-4 weeks group after 4 weeks of training, EAE was induced and the training was stopped. Results: Klotho and PLP levels increased in the EAE-EX1 group compared to the EAE and EAE-EX2 groups (P≤0.0001). Also, demyelination levels decreased in the EAE-EX1 group compared to the EAE group (P≤0.007). TNF-α concentration decreased in both EAE-exercise groups (P≤0.0001). Conclusion: The results of this study showed that HIIT increased Klotho and PLP levels and improved clinical outcomes in the in mice with MS.