مطالعات تشريحي دمبرگ در جنس زبان در قفا در ايران

Anatomical study on the petiole of Consolida species in Iran

ﺟﻨﺲ زﺑﺎن در ﻗﻔﺎ ) ( Consolidaاز ﺗﯿﺮه آﻻﻟﻪ در ﻃﺎﯾﻔﻪ Delphinieaeﻗﺮار دارد. اﯾﻦ ﺟﻨﺲ ﺷﺎﻣﻞ 52 ﮔﻮﻧﻪ ﺑﻮده و اﻋﻀﺎي ﺟﻨﺲ Aconitella را ﻧﯿﺰ در ﺑﺮ ﻣﯽ ﮔﯿﺮد. زﺑﺎن در ﻗﻔﺎ ﺑﺎ 24 ﮔﻮﻧﻪ در اﯾﺮان ﯾﮑﯽ از ﭘﺮ ﮔﻮﻧﻪ ﺗﺮﯾﻦ ﮐﺸﻮر ﻫﺎ در ﺟﻨﻮب ﻏﺮب آﺳﯿﺎ ﻣﺤﺴﻮب ﻣﯽ ﺷﻮد. در اﯾﻦ ﺗﺤﻘﯿﻖ وﯾﮋﮔﯽ ﻫﺎي ﺗﺸﺮﯾﺤﯽ دﻣﺒﺮگ 16 ﮔﻮﻧﻪ از ﺟﻨﺲ زﺑﺎن در ﻗﻔﺎ ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. از ﻫﺮ ﮔﻮﻧﻪ ﺳﻪ ﺟﻤﻌﯿﺖ ﺑﺮرﺳﯽ ﺷﺪ. ﺑﻌﺪ از ﭘﯿﺶ ﺗﯿﻤﺎر ﻧﻤﻮﻧﻪ ﻫﺎ، ﺑﺮش ﻫﺎي دﺳﺘﯽ از دﻣﺒﺮگ ﮔﺮﻓﺘﻪ ﺷﺪ و ﺑﺮش ﻫﺎ ﺗﻮﺳﻂ رﻧﮓ ﻫﺎي ﮐﺎرﻣﻦ و ﺳﺒﺰ ﻣﺘﯿﻞ رﻧﮓ آﻣﯿﺰي ﺷﺪﻧﺪ. از ﻧﻤﻮﻧﻪ ﻫﺎ ﺗﻮﺳﻂ ﻣﯿﮑﺮوﺳﮑﻮپ ﻧﻮري اوﻟﯿﻤﭙﻮس ﻋﮑﺲ ﺑﺮداري ﺷﺪ. ﻧﺘﺎﯾﺞ ﻣﺎ ﻧﺸﺎن داد ﮐﻪ وﺟﻮد ﮔﻮﺷﻪ ، ﺣﻀﻮر ﯾﮏ ﻻﯾﻪ ﮐﻼﻧﺸﯿﻢ در زﯾﺮ اﭘﯿﺪرم، ﻧﻮع و ﺗﻌﺪاد دﺳﺘﺠﺎت آوﻧﺪي وﺟﻮد ﺑﺎﻓﺖ ﻣﮑﺎﻧﯿﮑﯽ ﺑﯿﻦ دﺳﺘﺠﺎت آوﻧﺪي و وﺟﻮد ﮐﺮك ﺻﻔﺎت ﮐﻠﯿﺪي در ﺗﺸﺨﯿﺺ ﮔﻮﻧﻪ ﻫﺎ ﻫﺴﺘﻨﺪ. ﺑﺮ اﺳﺎس ﻣﻄﺎﻟﻌﺎت ﺗﺸﺮﯾﺤﯽ دﻣﺒﺮگ ﮔﻮﻧﻪ ﻫﺎي دو زﯾﺮﺟﻨﺲ Consolida Aconitella , از ﯾﮑﺪﯾﮕﺮ ﺟﺪا ﺷﺪه و در دو ﮔﺮوه ﻗﺮار ﮔﺮﻓﺘﻨﺪ ﮐﻪ ﺗﺎﯾﯿﺪ ﮐﻨﻨﺪه ﻧﺘﺎﯾﺞ ﻣﻄﺎﻟﻌﺎت ﺳﯿﺴﺘﻤﺎﺗﯿﮏ ﻣﻮﻟﮑﻮﻟﯽ در ﺟﻨﺲ است

The genus Consolida(DC.) S. F. GRAY of Gray is a member of Ranuculaceae family, Delphinieae tribe.. It comprises approximately 52 species which almost all of them are weed and includes the members of the genus Aconitella Spach. The genus distributed in Iran with 24 species, Iran is thus one of the richest countries for the genus in South-West Asia. Consolida has separated from Delphinium by De Candolle based on single-spurred petals , one follicle and annual life cycle and has occurred in separate section. Finally it introduced as a separate genus by Gray in 1821. In this study the petiole anatomy of 16 species of the genus Consolida have investigated. Three population from each species have examined. After pre-treatment of the material , hand sections prepared and Carmen and methyl green solution used for staining the materials. Olympus light microscope used for study and photography. Our results showed that the presence of : angle, collenchymas layer below the epidermis, the number of vascular bundles LB, vascular sheath type, mechanical tissue between the vascular bundles and type of vascular bundles and indumentums of petiole are diagnostic characters for distinguishing the species. Based on anatomical results the species of two subgenera (Consolida and Aconitella) have separated and this confirmed the results of molecular systematic study of the genus.