مقايسه ريخت‌سنجي هندسي ماهي تيله‌خوس (Capoeta capoeta) در سه حوضه ايران

Geometric morphometric comparison of teilehkhus fish (Capoeta capoeta) in three basins of Iran

ﺑﻪ ﻣﻨﻈﻮر ﻣﻘﺎﯾﺴﻪ رﯾﺨﺖﺳﻨﺠﯽ ﺟﻤﻌﯿﺖﻫﺎي ﺗﯿﻠﻪﺧﻮس Capoeta capoeta 713 ﻗﻄﻌﻪ ﻣﺎﻫﯽ از 32 رودﺧﺎﻧﻪ ﺣﻮﺿﻪ ﺧﺰر، اروﻣﯿﻪ و ﻫﺮﯾﺮود ﻣﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﺮﻓﺖ. ﻧﻤﻮﻧﻪﻫﺎ ﭘﺲ از ﺻﯿﺪ، ﺗﻮﺳﻂ ﻣﺤﻠﻮل ﮔﻞ ﻣﯿﺨﮏ 1٪ ﺑﯿﻬﻮش و در ﻓﺮﻣﺎﻟﯿﻦ 10% ﺗﺜﺒﯿﺖ و ﺑﻪ آزﻣﺎﯾﺸﮕﺎه اﻧﺘﻘﺎل ﯾﺎﻓﺘﻨﺪ. ﺗﺼﺎﯾﺮ دﯾﺠﯿﺘﺎل ﺑﺎ اﺳﺘﻔﺎده از دورﺑﯿﻦ ﻫﺸﺖ ﻣﮕﺎﭘﯿﮑﺴﻠﯽ ﺑﺎ ﺷﺮاﯾﻂ ﯾﮑﺴﺎن ﺗﻬﯿﻪ و ﺑﺎ اﺳﺘﻔﺎده از ﻧﺮماﻓﺰار Tpsdig2 ﻟﻨﺪﻣﺎرك ﮔﺬاري ﺷﺪﻧﺪ. روي ﻫﻤﮕﺬاري ﻟﻨﺪﻣﺎركﻫﺎ ﺑﺎ اﺳﺘﻔﺎده از ﺗﺤﻠﯿﻞ ﭘﺮوﮐﺮاﺳﺖ و ﺣﺬف ﺗﻐﯿﯿﺮات ﻏﯿﺮﺷﮑﻞ ﺑﺎ ﻧﺮماﻓﺰار TpsRelw اﻧﺠﺎم ﺷﺪ. ﻧﺘﺎﯾﺞ ﺗﺤﻠﯿﻞ ﻣﺘﻐﯿﺮﻫﺎي ﮐﺎﻧﻮﻧﯽ ﻧﺸﺎن داد ﮐﻪ ﺗﻔﺎوت ﻣﻌﻨﯽداري ﺑﯿﻦ ﺟﻤﻌﯿﺖﻫﺎي ﺳﻪ ﺣﻮﺿﻪ وﺟﻮد دارد و ﺟﻤﻌﯿﺖ ﻫﺮﯾﺮود ﮐﺎﻣﻼً از ﺟﻤﻌﯿﺖ اروﻣﯿﻪ ﺗﻔﮑﯿﮏ ﺷﺪ. ﺟﻤﻌﯿﺖ ﺧﺰر ﺑﺎ ﻫﺮ دو ﺟﻤﻌﯿﺖ ﻫﻢﭘﻮﺷﺎﻧﯽ ﻧﺸﺎن داد. ﺗﺤﻠﯿﻞ ﺧﻮﺷﻪاي ﺟﻤﻌﯿﺖﻫﺎ ﺑﺎ ﺿﺮﯾﺐ ﮐﻮﭘﺮﻧﯿﮏ 0/818، ﺟﻤﻌﯿﺖﻫﺎي ﻣﻮرد ﻣﻄﺎﻟﻌﻪ را ﺑﻪ دو ﮔﺮوه اﺻﻠﯽ ﺗﻘﺴﯿﻢ ﮐﺮد، ﺧﺰر و ﻫﺮﯾﺮود در ﯾﮏ ﮔﺮوه و اروﻣﯿﻪ در ﯾﮏ ﮔﺮوه ﻣﺠﺰا ﮐﻪ ﻧﺸﺎن دﻫﻨﺪه ﺷﺒﺎﻫﺖ ﺑﯿﺸﺘﺮ ﺣﻮﺿﻪ ﺧﺰر و ﻫﺮﯾﺮود ﺑﻪ ﻫﻤﺪﯾﮕﺮ اﺳﺖ. ﻓﺎﺻﻠﻪ ﺟﻐﺮاﻓﯿﺎﯾﯽ و ﺗﻔﺎوتﻫﺎي زﯾﺴﺘﮕﺎﻫﯽ ﻣﯽﺗﻮاﻧﺪ ﻋﻠﺖ اﯾﻦ اﻣﺮ ﺑﺎﺷﺪ، ﺑﺎ اﯾﻦ وﺟﻮد ﺑﺮاي ﻧﺘﯿﺠﻪﮔﯿﺮي دﻗﯿﻖﺗﺮ ﻧﯿﺎز ﺑﻪ ﻣﻄﺎﻟﻌﺎت ﻣﻮﻟﮑﻮﻟﯽ ﻣﯽﺑﺎﺷﺪ

In order to compare the geometric morphometrics of teilehkhus (Capoeta capoeta) populations, 713 specimens from 32 rivers in Caspian Sea, Urmia and Harirud basins were examined. Specimens were anesthetized in 1% clove oil solution after catching by a seine net and fixed in 10% formalin and transfered to laboratory for further studies. The specimens were photographed in a sililar condition by a Cannon 8MP and land marked in Tpsdig2 software. Superimposition of the landmarks was performed by Procrustes analysis to eliminate non-shape variations in TpsRelw. The Canonical Variate Ana;ysis (CVA) results of geometric morphometry showed significant differences among the populations and Harirud population was fully separated from Urmia populations. Caspia Sea population overlapped the both other populations. The Cluster Analysis (CA) grouped Harirud and Caspian Sea populations as a separate group from Urmia population, indication a more similarity between the Harirud and Caspian Sea populations. However, for more details, molecular studies are needed.