بررسي شاخص‌هاي فيزيولوژيكي و بيوشيميايي برخي رقم‌هاي سويا تحت تنش كادميوم

Study of physiological and biochemical parameters of some soybean (Glycine max) varieties (Telar, DPX, Caspian) under cadmium stress

در اﯾﻦ ﭘﮋوﻫﺶ، اﺛﺮات ﺗﯿﻤﺎر ﮐﺎدﻣﯿﻮم )200 ،0 و 400 ﻣﯿﮑﺮوﻣﻮﻻر( ﺑﺮ رﺷﺪ رﯾﺸﻪ و ﺳﺎﻗﻪ ، ﻣﺤﺘﻮاي ﻣﺎﻟﻮنديآﻟﺪﺋﯿﺪ، ﻓﻨﻞﮐﻞ، ﭘﺮاﮐﺴﯿﺪﻫﯿﺪروژن، ﮐﻠﺮوﻓﯿﻞ، آﻧﺘﻮﺳﯿﺎﻧﯿﻦ و ﻓﻌﺎﻟﯿﺖ آﻧﺰﯾﻢ ﻓﻨﯿﻞ آﻻﻧﯿﻦ آﻣﻮﻧﯿﺎﻟﯿﺎز )PAL( در ﺳﻪ رﻗﻢ ﺳﻮﯾﺎ ﺷﺎﻣﻞ ﺗﻼر، ﮐﺎﺳﭙﯿﻦ، DPX ﮔﺰارش ﺷﺪ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﺗﯿﻤﺎر ﮐﺎدﻣﯿﻮم ﺑﺎﻋﺚ ﮐﺎﻫﺶ ﭘﺎراﻣﺘﺮﻫﺎي رﺷﺪ رﯾﺸﻪ و ﺳﺎﻗﻪ و ﻫﻤﭽﻨﯿﻦ ﻣﺤﺘﻮاي ﮐﻠﺮوﻓﯿﻞ در رﻗﻢ ﻫﺎي ﺗﻼر، ﮐﺎﺳﭙﯿﻦ، DPX ﺷﺪه اﺳﺖ ﮐﻪ اﯾﻦ ﮐﺎﻫﺶ در رﻗﻢ ﺗﻼر ﭼﺸﻢﮔﯿﺮﺗﺮ ﺑﻮد. ﻣﺤﺘﻮاي آﻧﺘﻮﺳﯿﺎﻧﯿﻦ، ﻓﻨﻞ ﮐﻞ و ﻓﻌﺎﻟﯿﺖ آﻧﺰﯾﻢ ﻓﻨﯿﻞآﻻﻧﯿﻦآﻣﻮﻧﯿﺎﻟﯿﺎز در ﺗﯿﻤﺎر ﮔﯿﺎﻫﺎن ﺑﺎ ﮐﺎدﻣﯿﻮم در ﺳﻪ رﻗﻢ ﺳﻮﯾﺎ اﻓﺰاﯾﺶ ﯾﺎﻓﺖ. ﻣﺤﺘﻮاي ﻣﺎﻟﻮنديآﻟﺪﺋﯿﺪ در ﻫﺮ ﺳﻪ رﻗﻢ در ﺗﯿﻤﺎر 400 ﻣﯿﮑﺮوﻣﻮﻻري ﮐﺎدﻣﯿﻮم ﻧﺴﺒﺖ ﺑﻪ ﺷﺎﻫﺪ، اﻓﺰاﯾﺶ ﻣﻌﻨﯽداري ﻧﺸﺎن داده اﺳﺖ.. ﻧﺘﺎﯾﺞ اﯾﻦ ﺗﺤﻘﯿﻖ ﻧﺸﺎن داد ﮐﻪ رﻗﻢ ﺗﻼر ﺗﺤﺖ ﺗﻨﺶ ﮐﺎدﻣﯿﻮم ﺣﺴﺎﺳﯿﺖ ﺑﯿﺸﺘﺮي ﻧﺴﺒﺖ ﺑﻪ رﻗﻢﻫﺎي ﮐﺎﺳﭙﯿﻦ و DPX دارد.

In the current study, the effect of different concentrations of cadmium (0, 200, 400μM) on root and shoot growth, malondialdehyde (MDA), total phenol, hydrogen peroxide (H2O2), chlorophyll and anthocyanin contents, phenylalanine ammonia lyase (PAL) activity were investigated in three varieties of soybean plant (Telar, DPX, Caspian). The results showed that Cd treatment caused reduction of root and shoot biomass, chlorophyll a content in all three cultivars with the highest reduction in Telar. Significant increase in total phenolics and anthocyanin contents and PAL activity was observed in all the three soybean cultivars with the Cd treatment. The content of MDA in all three cultivars was significantly increased in 400µM concentration of Cd treatment as compared to control. The results of this study indicated that Telar cultivar was the most sensitive cultivare to cadmium stress compared with Caspian and DPX cultivars.