تأثير تمرينات تداومي با شدت متوسط بر بيان پروتئين گيرندۀ وانيلوئيدي نوع 1 در عضلات كندانقباض رت‌هاي نژاد ويستار

The Effect of Moderate Continuous Training on TRPV1 Protein Expression in Slow-Contraction Muscles of Wistar Rats

ﮔﯿﺮﻧﺪة واﻧﯿﻠﻮﺋﯿﺪي ﻧﻮع 1 )TRPV1( از ﮔﯿﺮﻧﺪهﻫﺎي ﻣﺮﺑﻮط ﺑﻪ درد در دﺳﺘﮕﺎه ﺣﺴﯽ اﺳﺖ ﮐﻪ ﺑﻪوﺳﯿﻠﮥ ﮐﭙﺴﺎﯾﺴﯿﻦ،ﮔﺮﻣﺎ، اﺳﯿﺪﯾﺘﻪ و ﺑﺮﺧﯽ ﻣﺘﺎﺑﻮﻟﯿﮏﻫﺎ ﻓﻌﺎل ﻣﯽﺷﻮد. ﻧﻘﺶ اﯾﻦ ﮔﯿﺮﻧﺪة ﮐﺎﺗﯿﻮﻧﯽ در اﯾﺠﺎد درد ﺷﻨﺎﺧﺘﻪ ﺷﺪه اﺳﺖ. ﮔﺰارشﻫﺎي ﺟﺪﯾﺪ از ﻧﻘﺶ TRPV1 در اﻋﻤﺎل دﯾﮕﺮ ﻧﯿﺰ ﺣﮑﺎﯾﺖ دارد و ﺗﺄﺛﯿﺮ اﯾﻦ ﮔﯿﺮﻧﺪه در رﻓﻠﮑﺲ ﻓﺸﺎر ورزﺷﯽ و ﭘﺎﺳﺦ ﻗﻠﺒﯽ- ﻋﺮوﻗﯽ در ﺑﺮﺧﯽ ﺑﯿﻤﺎريﻫﺎ در ﺣﺎل ﺑﺮرﺳﯽ اﺳﺖ. اﯾﻦ ﺗﺤﻘﯿﻖ ﺑﺎ روش ﺗﺠﺮﺑﯽ و ﺑﺎ ﻫﺪف رﺳﯿﺪن ﺑﻪ ﭘﺎﺳﺨﯽ ﻣﻨﺎﺳﺐ ﺑﺮاي درك ﺗﺄﺛﯿﺮ ﺗﻤﺮﯾﻨﺎت ﺗﺪاوﻣﯽ ﺑﺎ ﺷﺪت ﻣﺘﻮﺳﻂ ﺑﺮ ﺑﯿﺎن ﭘﺮوﺗﺌﯿﻦ TRPV1 در ﻋﻀﻠﮥ ﻧﻌﻠﯽ ﻃﺮح ﺷﺪ. ﺑﺪﯾﻦﻣﻨﻈﻮر 16 ﺳﺮ رت ﻧﺮ ﻧﮋاد وﯾﺴﺘﺎر ﺑﺎ وزن 200-220 ﮔﺮم، در دو ﮔﺮوه، ﺷﺎﻣﻞ ﯾﮏ ﮔﺮوه ﺗﻤﺮﯾﻦ و ﯾﮏ ﮔﺮوه ﮐﻨﺘﺮل، ﺑﻪﺻﻮرت ﺗﺼﺎدﻓﯽ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﮔﺮوه ﺗﻤﺮﯾﻦ 6 ﻫﻔﺘﻪ و ﻫﺮ ﻫﻔﺘﻪ 5 ﺟﻠﺴﻪ ﺗﻤﺮﯾﻦ ﻣﺪاوم ﺑﺎ ﺷﺪت ﻣﺘﻮﺳﻂ اﻧﺠﺎم دادﻧﺪ و در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﮔﺮوه ﮐﻨﺘﺮل ﮐﻪ در ﺗﻤﺎم ﺷﺮاﯾﻂ ﻣﺸﺎﺑﻪ ﮔﺮوه ﺗﻤﺮﯾﻦ ﺑﻮد وﻟﯽ ﻫﯿﭻ ﺗﻤﺮﯾﻦ ورزﺷﯽ اﻧﺠﺎم ﻧﻤﯽدادﻧﺪ، ﺑﺮرﺳﯽ ﺷﺪﻧﺪ. رتﻫﺎ ﺗﻮﺳﻂ اﺻﻮل اﺳﺘﺎﻧﺪارد ﺑﯽﻫﻮش، ﻗﺮﺑﺎﻧﯽ و ﺑﺎﻓﺖﻫﺎي ﻣﻮردﻧﻈﺮ اﺳﺘﺨﺮاج ﺷﺪ. ﯾﺎﻓﺘﻪﻫﺎي آﻣﺎري از ﺧﺮوﺟﯽ آزﻣﻮن t ﻣﺴﺘﻘﻞ ﻣﺴﺘﺨﺮج از ﯾﺎﻓﺘﻪﻫﺎي آزﻣﺎﯾﺸﮕﺎﻫﯽ وﺳﺘﺮن ﺑﻼﺗﯿﻨﮓ ﺑﺮاي ﻣﻘﺎدﯾﺮ ﺑﯿﺎن ﭘﺮوﺗﺌﯿﻦ ﮔﯿﺮﻧﺪة TRPV1 ﻧﺸﺎن داد ﮐﻪ در ﮔﺮوه ﺗﻤﺮﯾﻦ اﻓﺰاﯾﺶ ﻣﻌﻨﺎداري )0/033=P( در ﺑﯿﺎن اﯾﻦ ﮔﯿﺮﻧﺪه ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل در ﻋﻀﻠﮥ ﻧﻌﻠﯽ ﺑﻪوﺟﻮد آﻣﺪه اﺳﺖ. اﯾﻦ ﻧﺘﺎﯾﺞ ﻧﺸﺎن ﻣﯽدﻫﻨﺪ ﺑﺎ اﻓﺰاﯾﺶ در ﺑﯿﺎن ﭘﺮوﺗﺌﯿﻦ ﮔﯿﺮﻧﺪه TRPV1، اﻋﺼﺎب ﺣﺴﯽ ﻧﺴﺒﺖ ﺑﻪ ﺗﻤﺮﯾﻨﺎت ورزﺷﯽ ﺳﺎزﮔﺎري ﭘﯿﺪا ﻣﯽﮐﻨﻨﺪ.

The vanilloid receptor 1 (TRPV1) is a nociceptor receptor in sensitive system which is activated by capsaicin, heat, acidity and some metabolites. The role of this cation receptor is known at pain. New reports suggest that TRPV1 plays a role in other processes and researchers are studying the effect of this receptor on exercise pressure reflex (EPR) and cardiovascular response in some diseases. In this experimental study, the aim of this study was to find an appropriate answer to perceive the effect of moderate continuous training on the expression of TRPV1 protein in soleus muscle. 16 male Wistar rats (weight: 200-220 gr) were randomly assigned to 2 groups: training and control. The training group performed 6 weeks (5 sessions per week) of moderate continuous training. The control group did not have any training but there was no difference between these groups in all conditions. The rats were anesthetized, sacrificed and their required tissues were extracted by standard procedures. The results of independent t test derived from Western Blotting laboratory findings for TRPV1 receptor protein expression showed a significant increase (P=0.033) in the expression of this receptor in soleus muscle in the training group compared with the control group. These results show that sensory nerves are adapted to training with an increase in TRPV1 receptor protein expression.