اثر 4 هفته تمرين هوازي بر سطوح سرمي و كبدي نئورگولين-4 موش‌هاي صحرايي ديابتي

The Effect of 4 Weeks of Aerobic Exercise on Serum and Liver Levels of Neuregulin-4 in Diabetic Rats

دﻳﺎﺑﺖ ﺑﺎ اﻓﺰاﻳﺶ ﻟﻴﭙﻮژﻧﺰ و ﺑﻴﺎن ژنﻫﺎي آن در ﻛﺒﺪ و ﻓﻌﺎﻟﻴﺖ ﻧﺌﻮرﮔﻮﻟﻴﻦ-4 ﺑﺎ ﺗﻀﻌﻴﻒ ﻟﻴﭙﻮژﻧﺰ ﻛﺒﺪي ﻫﻤﺮاه اﺳﺖ. ﻫﺪف ﭘﮋوﻫﺶ ﺣﺎﺿﺮ ﺑﺮرﺳﻲ ﺗﺄﺛﻴﺮ 4 ﻫﻔﺘﻪ ﺗﻤﺮﻳﻦ ﻫﻮازي ﺑﺮ ﺗﻐﻴﻴﺮات ﺳﻄﻮح ﺳﺮﻣﻲ و ﻛﺒﺪي ﻧﺌﻮرﮔﻮﻟﻴﻦ-4 و ﻧﻴﻤﺮخ ﻟﻴﭙﻴﺪي ﻣﻮشﻫﺎي دﻳﺎﺑﺘﻲ ﺑﻮد. 32 ﺳﺮ ﻣﻮش ﺻﺤﺮاﻳﻲ ﻧﺮ ﺑﺎﻟﻎ، ﺑﻪﺻﻮرت ﺗﺼﺎدﻓﻲ ﺑﻪ ﮔﺮوهﻫﺎي ﻫﺸﺖﺗﺎﻳﻲ ﻛﻨﺘﺮل ﺳﺎﻟﻢ، ﻛﻨﺘﺮل دﻳﺎﺑﺘﻲ، ﺗﻤﺮﻳﻦ و دﻳﺎﺑﺘﻲ ﺗﻤﺮﻳﻦ ﺗﻘﺴﻴﻢ ﺷﺪﻧﺪ. اﻟﻘﺎي دﻳﺎﺑﺖ ﺑﺎ ﺗﺰرﻳﻖ درونﺻﻔﺎﻗﻲ ﻣﺤﻠﻮل اﺳﺘﺮﭘﺘﻮزوﺗﻮﺳﻴﻦ )mg/kg 55( اﻧﺠﺎم ﮔﺮﻓﺖ. ﻓﺮاﻳﻨﺪ ﺗﻤﺮﻳﻦ ﺑﻪ ﻣﺪت 4 ﻫﻔﺘﻪ )5 ﺟﻠﺴﻪ در ﻫﻔﺘﻪ( ﺑﺎ ﺳﺮﻋﺖ 15 ﺗﺎ 18 ﻣﺘﺮ در دﻗﻴﻘﻪ و ﺑﺮاي 25 ﺗﺎ 44 دﻗﻴﻘﻪ اﺟﺮا ﺷﺪ. 48 ﺳﺎﻋﺖ ﭘﺲ از آﺧﺮﻳﻦ ﺟﻠﺴﺔ ﺗﻤﺮﻳﻨﻲ، ﺧﻮنﮔﻴﺮي و ﺑﺎﻓﺖﺑﺮداري اﻧﺠﺎم ﮔﺮﻓﺖ و ﺳﻄﻮح ﻧﺌﻮرﮔﻮﻟﻴﻦ-4 اﻧﺪازهﮔﻴﺮي ﺷﺪ. ﺗﻔﺎوﺗﻲ در ﺳﻄﻮح ﺳﺮﻣﻲ و ﻛﺒﺪي ﻧﺌﻮرﮔﻮﻟﻴﻦ-4 ﺑﻴﻦ ﮔﺮوهﻫﺎي دﻳﺎﺑﺘﻲ و ﻏﻴﺮدﻳﺎﺑﺘﻲ ﻣﺸﺎﻫﺪه ﻧﺸﺪ )0/05>P(. ﺳﻄﻮح ﺳﺮﻣﻲ ﻛﻠﺴﺘﺮول و HDL ﺑﻴﻦ ﮔﺮوهﻫﺎي ﻣﺬﻛﻮر ﺗﻔﺎوت ﻣﻌﻨﺎداري ﻧﺪاﺷﺖ )0/05>P(. ﺗﺮيﮔﻠﻴﺴﺮﻳﺪ و LDL ﺑﻴﻦ اﻳﻦ ﮔﺮوهﻫﺎ ﺑﺎ اﻓﺰاﻳﺶ ﻣﻌﻨﺎدار ﻫﻤﺮاه ﺑﻮد )0/05P(. ﻫﻤﭽﻨﻴﻦ 4 ﻫﻔﺘﻪ ﺗﻤﺮﻳﻦ ﻫﻮازي ﺑﺮ ﺳﻄﻮح ﺳﺮﻣﻲ ﻛﻠﺴﺘﺮول، ﺗﺮيﮔﻠﻴﺴﺮﻳﺪ، HDL و LDL در ﻣﻮشﻫﺎي ﺳﺎﻟﻢ ﻣﺆﺛﺮ ﻧﺒﻮد )0/05>P(. ﺗﻤﺮﻳﻦ در ﻣﻮشﻫﺎي دﻳﺎﺑﺘﻲ ﻣﻮﺟﺐ ﻛﺎﻫﺶ ﻣﻌﻨﺎدار ﺳﻄﻮح ﺳﺮﻣﻲ ﻛﻠﺴﺘﺮول و ﺗﺮيﮔﻠﻴﺴﺮﻳﺪ ﺷﺪ )0/05P(. ﺑﺎ اﻳﻨﻜﻪ ﺳﻄﻮح ﻧﻴﻤﺮخ ﻟﻴﭙﻴﺪي دﭼﺎر ﺗﻐﻴﻴﺮات ﻣﺤﺴﻮس ﺷﺪ، اﺣﺘﻤﺎﻻً ﺗﻤﺮﻳﻨﺎت ورزﺷﻲ ﻫﻮازي در 4 ﻫﻔﺘﻪ ﻧﻤﻲﺗﻮاﻧﺪ ﻣﺤﺮك اﻓﺰاﻳﺶ ﻧﺌﻮرﮔﻮﻟﻴﻦ-4 ﮔﺮدﺷﻲ و ﺑﺎﻓﺘﻲ ﻛﺒﺪ ﺑﺎﺷﺪ

Diabetes is associated with increased lipogenesis and expression of lipogenic genes in the liver, and neuregulin-4 activity is associated with inhibition of liver lipogenesis. The aim of this study was to investigate the effect of 4 weeks of aerobic exercise on changes of serum and liver levels of neuregulin-4and lipid profile in diabetic rats. ­32 male adult rats were randomly divided into healthy control, control diabetic, exercise and exercise diabetic groups (each group 8 rats). Diabetes was induced by intraperitoneal injection of streptozotocin solution (55 mg/kg). The exercise was performed for 4 weeks, 5 sessions per week, at speeds of 15 to 18 m/min. and for 25 to 44 minutes. 48 hours after the last exercise session, blood samples were collected and liver tissue was removed and Neuregulin-4 levels were measured. The results showed no difference in serum and liver levels of neuregulin-4 between diabetic and healthy groups (P>0.05). Serum levels of cholesterol and HDL were not significantly different among the above groups (P>0.05). Triglyceride and LDL were significantly higher among these groups (P<0.05). 4 weeks of aerobic exercise did not affect serum and liver levels of neuregulin-4 in diabetic and healthy rats (P>0.05). Also, 4 weeks of aerobic exercise did not affect serum levels of cholesterol, triglyceride, HDL and LDL in healthy rats (P>0.05). Exercise in diabetic rats significantly decreased serum levels of cholesterol and triglyceride (P<0.05) but it did not make any significant differences in HDL and LDL (P>0.05). ­Although the levels of lipid profile changed significantly, 4 weeks of aerobic exercise probably cannot stimulate increases of neuregulin-4 in serum and liver tissue.