پديد آورندگان :
تيماس، امير دانشگاه محقق اردبيلي، اردبيل , سيف دواتي، جمال دانشگاه محقق اردبيلي - گروه علوم دامي، اردبيل , سيف زاده، صياد دانشگاه محقق اردبيلي، اردبيل , عبدي بنمار، حسين دانشگاه محقق اردبيلي - گروه علوم دامي، اردبيل , شريفي، رضا دانشگاه محقق اردبيلي - گروه علوم دامي، اردبيل
كليدواژه :
وتيرات مونوگليسريد , پروبيوتيك , فراسنجههاي خوني , گوساله هلشتاين
چكيده لاتين :
Introduction4 Probiotic supplements improve livestock growth, increase in intake of feed, improve digestion and
absorption of nutrients and health conditions. Feeding butyrate in combination with milk and starter prior to weaning of
calves may enhance rumen development, body weight, health status and growth performance. The butyrate supplement
used in this study is monoglyceride butyrate and during digestion of ruminal fat, first glycerol is separated from the
glyceride composition, and then the isolated bacteria are exposed to digestion and absorption of butyric acid. Butyric
acid is one of the absorbed volatile fatty acids and metabolism of the rumen wall, the final product of which is
metabolized by the rumen stem cell epithelial cells, a beta-hydroxy butyrate compound. The aim of this study was to
evaluate effect of butyrate monoglyceride and probiotic additives on growth performance, some blood parameters and
digestibility of nutrients in Holstein suckling calves.
Materials and Methods For this experiment, 24 newborn Holstein calves with an average age of 6 days, and
about 36±2 kg weight, with 4 treatments and 6 replications were performed in a completely randomized factorial design
(2×2). The treatments included: 1) base rations (starter and whole milk) 2) base ration + daily 2 grams of probiotic
(Protexin) mixed with milk 3) base rations + daily ration 5 grams of butyrate monoglyceride mixed with starter 4) base
ration + 2 grams of periobiotic mixed in milk + 5 grams of monoglyceride butyrate mixed with starter. During the
experiment, functional traits such as daily gain, daily intake and feed conversion were determined. Calves were weighed
individually on days 30 and 65. In order to measure apparent digestibility at the end of the course, the feces were
collected for 5 consecutive days. The collected samples were dried in an oven at 65 ° C for 48 hours. During the 30th
and 65th days of the experiment, blood samples were taken from the vein before the morning meal. Blood samples were
transferred to the laboratory for centrifugation (at 3500 rpm) for 15 minutes. The serum samples were stored at -20 ° C
until measured. Blood glucose, urea, cholesterol, triglyceride, albumin, total protein and beta-hydroxy butyric acid were
measured using laboratory kits (Pars Test, Iran) and using the CS-400 auto-analyzer. Data were analyzed using MIXED
and SAS software.
Results and Discussion The results showed that final weight was not affected by probiotic and probiotic and
butyrate monoglyceride interacting (P>0.05). But it was affected by the butyrate monoglyceride (P<0.05). Daily feed
intake was not affected by experimental treatments (P>0.05). Daily weight gain was not affected by probiotic and
probiotic and butyrate monoglyceride interactions (P>0.05). But it was affected by the butyrate monoglyceride
(P<0.05). Feed conversion ratio was not affected by treatments during the whole period (P>0.05). But in the first month
it was affected by the probiotic (P<0.05). The results of this study showed that the concentration of beta-hydroxy
butyric blood was not affected by the probiotic factor (P<0.05), while the butyrate supplementation factor was effective
in increasing the concentration of beta-hydroxy-butyric (P<0.05). Also, there was a tendency for the probabiotic and
monoglyceride butyrate interactions to be meaningful (P<0.055). The level of beta-hydroxybutyric acid indicates rumen
development and the beginning of the metabolism of the final products of fermentation by rumen epithelium The results
showed that digestion of calves was not affected by experimental diets (P>0.05). The interaction factor of probiotic and
butyrate monoglyceride had a significant effect on blood glucose concentration (P<0.05). Also, beta-hydroxy butyric
acid is affected by butyrate supplementation (P<0.05). However, experimental treatments of butyrate and probiotic
(Protexin) had no effect on cholesterol, triglyceride, albumin, and total protein (P>0.05).
Conclusion The probiotic (Protexin) and the interaction factor of probiotic and butyrate monoglyceride did not affect
daily intake, daily gain, final body weight of calves and feed conversion ratio, while the addition of butyrate
monoglyceride in the diet affected the weight gain and calving weight of the calves daily. Nutrient utilization and
digestibility of dairy calves was not affected by experimental diets. Beta-hydroxy-butyric acid was also affected by
butyrate supplementation. However, experimental treatments of butyrate and probiotic (Protexin) had no effect on
cholesterol, triglyceride, albumin, and total protein. In general, supplementation of the diet of infected calves with
butyrate monoglycerides can have beneficial effects on the performance and development of rumen in infant calves. In
general, it can be concluded that butyrate monoglyceride as supplementation of the diet (milk replacer or starter diet)
may enhance rumen development in Holstein suckling calves.
