اثر هشت هفته تمرين تناوبي شديد بر فعاليت سيستم يوبي كويتين – پروتئازوم در عضلة تند انقباض موش‌هاي صحرايي پس از انفاركتوس ميوكارد

The Effect of Eight Weeks of High Intensity Interval Training on Ubiquitin-Proteasome System in Fast Twitch Muscle of Post Myocardial Infarction Rats

و آﺗﺮوﻓﯽ ﻋﻀﻼﻧﯽ اﺷﺎره ﮐﺮد؛ ﺑﻨﺎﺑﺮاﯾﻦ، ﻫﺪف از اﻧﺠﺎم ﭘﮋوﻫﺶ ﺣﺎﺿﺮ، ﺑﺮرﺳﯽ ﺗﺄﺛﯿﺮ ﻫﺸﺖ ﻫﻔﺘﻪ ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ )HIIT( ﺑﺮ ﻓﻌﺎﻟﯿﺖ ﺳﯿﺴﺘﻢ ﯾﻮﺑﯽ ﮐﻮﯾﺘﯿﻦ-ﭘﺮوﺗﺌﺎزوم در ﻋﻀﻠﮥ ﺗﻨﺪ اﻧﻘﺒﺎض ﻣﻮشﻫﺎي ﺻﺤﺮاﯾﯽ ﭘﺲ از اﻧﻔﺎرﮐﺘﻮس ﻣﯿﻮﮐﺎرد )MI( ﺑﻮد. MI ﺑﺎ ﺑﺴﺘﻦ ﺷﺮﯾﺎن ﮐﺮوﻧﺮي ﻧﺰوﻟﯽ ﭼﭗ LAD)( ﺑﻪﻣﺪت 30 دﻗﯿﻘﻪ اﻧﺠﺎم ﺷﺪ. 32 ﻣﻮش ﺻﺤﺮاﯾﯽ ﻧﺮ ﻧﮋاد وﯾﺴﺘﺎر ﺑﻪﻃﻮر ﺗﺼﺎدﻓﯽ ﺑﻪ ﭼﻬﺎر ﮔﺮوه ﮐﻨﺘﺮل MI-CTL) MI(، ﮔﺮوه MI-HIIT، ﮔﺮوه ﮐﻨﺘﺮل HIIT و ﮔﺮوه ﮐﻨﺘﺮل ﺟﺮاﺣﯽ )Sham( ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ ﺑﻪﻣﺪت ﻫﺸﺖ ﻫﻔﺘﻪ، ﺳﻪ روز در ﻫﻔﺘﻪ و ﻫﺮ ﺟﻠﺴﻪ 30 دﻗﯿﻘﻪ دوﯾﺪن ﺑﺎ ﺷﺪت 85 ﺗﺎ 90 درﺻﺪ Vo2max اﻧﺠﺎم ﺷﺪ. 24 ﺳﺎﻋﺖ ﺑﻌﺪ از آﺧﺮﯾﻦ ﺟﻠﺴﮥ ﺗﻤﺮﯾﻨﯽ رتﻫﺎ ﺑﯽﻫﻮش ﺷﺪﻧﺪ و ﺑﺎﻓﺖ ﻋﻀﻠﮥ ﺑﺎزﮐﻨﻨﺪة ﻃﻮﯾﻞ اﻧﮕﺸﺘﺎن ﭘﺎ )EDL( ﺧﺎرج ﺷﺪ. ﺑﺮاي ﺳﻨﺠﺶ ﺑﯿﺎن ﻣﺘﻐﯿﺮﻫﺎ )1-TNF-a ،NF-KB ،P38 MAPK ،Murf و NOX2( از روش RT-PCR اﺳﺘﻔﺎده ﺷﺪ. ﻫﻤﭽﻨﯿﻦ، از آزﻣﻮن آﻣﺎري ﺗﺤﻠﯿﻞ وارﯾﺎﻧﺲ ﯾﮏراﻫﻪ و آزﻣﻮن ﺗﻌﻘﯿﺒﯽ ﺗﻮﮐﯽ ﺑﺮاي ﺗﺤﻠﯿﻞ دادهﻫﺎ اﺳﺘﻔﺎده ﺷﺪ )0.05

Weakness, intolerance of exercise and eventually muscle atrophy are important features of patients with heart failure. Therefore, the purpose of this study was to investigate the effect of 8 weeks of high intensity interval training on ubiquitin-proteasome system in fast twitch muscle of post myocardial infarction (MI) rats. Methods: Myocardial infarction was induced by left artery descending ligation for 30 min. 32 male Wistar rats were randomly divided into 4 groups (n = 8): MI control (MI-CTL), high-intensity interval MI (MI-HIIT), high-intensity interval (HIIT) and Sham- operated. Exercise training was undergoing 40 min, 3 sessions per week for 8-weeks (85-90% VO2max). 24 hours after the last training session, the rats were anesthetized and their extensor digitorum longus (EDL) tissue removed. RT-PCR method was used to measure gene expression (NOX2, TNF-a, NF-KB, P38 MAPK, Murf-1) was used. Data analysis were performed using a one-way ANOVA and Tukey follow up tests, considering as significance level (P < 0.05) . Results: 8-week HIIT significantly decreased NOX2, TNF-a in MI-HIIT compared with MI-CTL and Sham (P=0.03). These reductions were paralleled a significant decrease in activity NF-KB, P38, Murf-1 in MI-HIIT compared with MI-CTL and Sham (P<0.05). Also, NOX2 change in HIIT group was significantly higher than MI-CTL and Sham (P<0.05). According to the results of HIIT probably as an effective treatment, it reduces muscle atrophy in fast muscle contraction of heart patients.