ارزيابي پارامترهاي فيزيكي پوست با پردازش تصاوير فراصوتي در طول تابش با نور فرابنفش در مدل حيواني چروك شدگي

Evaluation of Physical Parameters of Skin by Consecutive Ultrasonic Image Processing During Ultraviolet Radiation in an Animal Model of Wrinkled Skin

ﭘﯿﺮي ﭘﻮﺳﺖ ﺑﻪ دو دﺳﺘﻪ ﭘﯿﺮي ذاﺗﯽ و ﭘﯿﺮي ﺧﺎرﺟﯽ ﺗﻘﺴﯿﻢ ﻣﯽ ﺷﻮد. ﭘﯿﺮي ﭘﻮﺳﺖ در اﺛﺮ ﻣﻮاﺟﻬﻪ ﻣﮑﺮر ﺑﺎ اﺷﻌﻪ ﻓﺮاﺑﻨﻔﺶ )ﭘﯿﺮي ﺧﺎرﺟﯽ( ﺑﺎ ﭘﯿﺮي ﮐﻪ در اﺛﺮ ﮔﺬﺷﺖ زﻣﺎن )ﭘﯿﺮي ﻣﺰﻣﻦ( اﯾﺠﺎد ﻣﯽ ﺷﻮد، ﻣﺘﻔﺎوت اﺳﺖ. ﭘﯿﺪاﯾﺶ ﭼﯿﻦ و ﭼﺮوك ﻫﺎي ﭘﻮﺳﺘﯽ ﻧﺎﺷﯽ از ﺗﺎﺑﺶ ﻧﻮر آﻓﺘﺎب ﺑﻪ دﻟﯿﻞ آﺗﺮوﻓﯽ ﭼﺮﺑﯽ ﻧﺎﺣﯿﻪ زﯾﺮ ﺟﻠﺪي و ﮐﺎﻫﺶ ﺗﻮﻟﯿﺪ ﮐﻼژن و اﻻﺳﺘﯿﻦ اﯾﺠﺎد ﻣﯽ ﺷﻮد و در ﻧﺘﯿﺠﻪ ﻣﻮﺟﺐ ﺗﻐﯿﯿﺮ ﺧﺼﻮﺻﯿﺎت ﺑﯿﻮﻣﮑﺎﻧﯿﮑﯽ ﺑﺎﻓﺖ ﭘﻮﺳﺖ ﻣﯽ ﮔﺮدد. اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﯽ آﺳﯿﺐ ﭘﻮﺳﺘﯽ ﻧﺎﺷﯽ از ﺗﺎﺑﺶ اﻣﻮاج ﻓﺮاﺑﻨﻔﺶ B ﺗﻮﺳﻂ ﭘﺮدازش ﺗﺼﺎوﯾﺮ ﻣﺘﻮاﻟﯽ ﻓﺮاﺻﻮﺗﯽ ﺑﺎ ﻗﺪرت ﺗﻔﮑﯿﮏ ﺑﺎﻻ اﻧﺠﺎم ﺷﺪ. ﻣﻮاد و روشﻫﺎ: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﺠﺮﺑﯽ ﺑﺮرﺳﯽ روﻧﺪ آﺳﯿﺐ ﭘﻮﺳﺘﯽ ﺗﻌﺪاد 25 ﺳﺮ ﻣﻮش C57BL6 در ﮔﺮوه ﻫﺎي ﺳﺎﻟﻢ )دوز ﺻﻔﺮ( و ﺗﺤﺖ ﺗﺎﺑﺶ اﻣﻮاج ﻓﺮاﺑﻨﻔﺶ ﺑﻪ ﺻﻮرت ﻫﻔﺘﮕﯽ، ﺑﻪ دﻟﯿﻞ ﺗﻔﺎوت در وﯾﮋﮔﯽ ﻫﺎي ﭘﻮﺳﺘﯽ ﺗﺤﺖ ﺗﺎﺑﺶ اﻣﻮاج UVB ﺑﺎ ﺷﺪت ﺗﺎﺑﺶ 0/03 ﻣﯿﻠﯽ وات ﺑﺮ ﺳﺎﻧﺘﯽ ﻣﺘﺮ ﻣﺮﺑﻊ )5 ﺑﺎر در ﻫﻔﺘﻪ ﺑﻪ ﻣﺪت 5 ﻫﻔﺘﻪ( ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﭘﺎراﻣﺘﺮﻫﺎي ﻓﯿﺰﯾﮑﯽ ﻻﯾﻪ ﻫﺎي ﭘﻮﺳﺘﯽ درم و اﭘﯽ درم ﺑﻪ ﺻﻮرت ﻫﻔﺘﮕﯽ از روز7 ﺗﺎ روز 35 ﺑﺎ اﺳﺘﻔﺎده از ﺗﺼﺎوﯾﺮ ﻓﺮاﺻﻮﺗﯽ اﻧﺪازه ﮔﯿﺮي و ﺑﺮرﺳﯽ ﺷﺪﻧﺪ. ﯾﺎﻓﺘﻪﻫﺎ: ﺿﺨﺎﻣﺖ ﻻﯾﻪ ﻫﺎي درم و اﭘﯽ درم ﺣﺎﺻﻞ از ﭘﺮدازش ﺗﺼﺎوﯾﺮ ﻓﺮاﺻﻮﺗﯽ در ﻃﻮل ﻓﺮآﯾﻨﺪ آﺳﯿﺐ ﺣﺎﺻﻞ از ﺗﺎﺑﺶ ﻓﺮاﺑﻨﻔﺶ در ﻣﺪل ﺣﯿﻮاﻧﯽ ﻣﻮش ﺑﻪ ﻃﻮر ﻣﻌﻨﯽ داري در ﻃﻮل 5 ﻫﻔﺘﻪ ﺑﺮرﺳﯽ، اﻓﺰاﯾﺶ ﯾﺎﻓﺖ )p<0/05(. ﻋﻼوه ﺑﺮ اﯾﻦ درﺻﺪ ﺗﻐﯿﯿﺮات ﺿﺨﺎﻣﺖ ﻻﯾﻪ اﭘﯽ درم )از 0/22±0/01 ﻣﯿﻠﯽ ﻣﺘﺮ در روز ﺻﻔﺮ ﺑﻪ 0/37±0/02 ﻣﯿﻠﯽ ﻣﺘﺮ در روز ﺳﯽ و ﭘﻨﺠﻢ( و ﻻﯾﻪ درم )از 0/57±0/05 ﻣﯿﻠﯽ ﻣﺘﺮ در روز ﺻﻔﺮ ﺑﻪ0/90±0/08 ﻣﯿﻠﯽ ﻣﺘﺮ در روز ﺳﯽ و ﭘﻨﺠﻢ( ﺑﻪ ﺗﺮﺗﯿﺐ 68 و 57 درﺻﺪ اﻓﺰاﯾﺶ را ﻧﺸﺎن داد. ﻧﺘﯿﺠﻪ ﮔﯿﺮي: ﻧﺘﺎﯾﺞ ﻣﻄﺎﻟﻌﻪ ﻧﺸﺎن داد ﮐﻪ ﺗﺎﺑﺶ اﻣﻮاج ﻓﺮاﺑﻨﻔﺶ B، ﻣﻮﺟﺐ اﻓﺰاﯾﺶ ﺿﺨﺎﻣﺖ ﻻﯾﻪ ﻫﺎي ﭘﻮﺳﺘﯽ ﻣﯽ ﺷﻮد.

Skin aging is divided into two categories of intrinsic and extrinsic aging. Skin aging due to repeated exposure to ultraviolet radiation (extrinsic aging) is different from aging caused by time (intrinsic aging). The appearance of wrinkles caused by sunlight is due to subcutaneous fat atrophy and reduced production of collagen and elastin, thereby altering the biomechanical properties of the skin tissue. This study was conducted to investigate the skin damage caused by ultraviolet B (UVB) radiation by consecutive ultrasonic image processing with high resolution. METHODS In this experimental study, we evaluated the skin injury process among 25 C57BL/6 mice in healthy group (zero dose), and case group exposed to UVB radiation at 0.03 milliwatts per square centimeter (5 times a week for 5 weeks) due to differences in skin characteristics. Physical parameters of dermal and epidermal layers were measured and evaluated weekly from day 7 to day 35 using ultrasonic image processing. FINDINGS The thickness of the dermal and epidermal layers obtained by ultrasonic processing during the process of ultraviolet radiation injury in the mouse model significantly increased during the 5 – week study (p < 0.05). In addition, the percentage of changes in the thickness of the epidermis layer (from 0.22±0.01 mm on day zero to 0.37 ± 0.02 mm on the thirty-fifth day) and the dermal layer (from 0.57 ± 0.05 on day zero to 0.90 ± 0.08 mm on the thirty-fifth day) showed 68% and 57% increase, respectively. CONCLUSION The results showed that UVB radiation increased the thickness of the skin layers.