مطالعه اثر ضد باكتريايي عصاره ريشه زرشك (Berberis vulgaris) و دانه رازيانه (Foeniculum vulgare) بصورت انفرادي و توام با نايسين و دي استات سديم بر باكتري يرسينيا انتروكوليتيكا

Study of antibacterial effects of barberry root (Berberis vulgaris) and fennel seed (Foeniculum vulgare) extracts individually and combined with nisin and sodium diacetate on Yersinia enterocolitica

اﻣﺮوزه بدليل ﺳﻨﺘﺰى و ﺷﯿﻤﯿﺎﯾﻰ ﺑﻮدن آنتي‌بيوتيك‌ها و ﻣﻘﺎوﻣﺖ ﺑﺪن ﻧﺴﺒﺖ ﺑﻪ آنها، اﺳﺘﻔﺎده از ﺗﺮﮐﯿﺒﺎت ﮔﯿﺎﻫﻰ ﺑﺮاى درﻣﺎن بيماري‌ها و عفونت‌ها بسيار موردتوجه قرار گرفته اﺳﺖ. در اين تحقيق، عصاره گيري از گياهان با روش خيساندن در اتانول 80 درصد انجام و عصاره ريشه زرشك با روش HPTLC و عصاره دانه رازيانه با استفاده ازGC/MS مورد آناليز قرار گرفت. براي اثر ضد ميكروبي در 3 تكرار از روش انتشار در آگار و ميكرودايلوشن استفاده شد. در بررسي حداقل غلظت بازدارندگي (MIC)، بين اثر ضد باكتريايي عصاره ريشه زرشك و دانه رازيانه به‌صورت انفرادي و توام با نايسين و دي استات سديم بر يرسينيا انتروكوليتيكا اختلاف معني‌داري مشاهده شد. در بين عوامل مورد بررسي، نايسين بيشترين اثر آنتي باكتريال و پس ‌از آن به ترتيب عصاره ريشه زرشك و دانه رازيانه اثر ضد باكتريايي خود را نشان دادند. اثر آنتي باكتريال دي استات سديم بر يرسينيا انتروكوليتيكا كمتر بود. اين نتايج با نتايج تست انتشار ديسك در آگار تا حد زيادي همخواني داشت. نتايج نشان داد MIC دي استات سديم و نايسين توام با عصاره هاي ريشه زرشك و دانه رازيانه كاهش قابل‌توجهي نسبت به حالت انفرادي هر يك به ‌تنهايي داشت (0/05

Due to the synthetic and chemical nature of antibiotics and the phenomenon of body resistance against them, using herbal compounds to cure infections and diseases are being noticed a lot, today. In this study, extracting the herbs was done by soaking in 80% ethanol. Berberis Vulgaris (Barberry root)by HPTLC and Foeniculum vulgare (F. vulgare). Mill (Fennel seed)by GC/MS methods were analyzed. Disk diffusion and micro dilution methods were used to detection of the antibacterial effect in triplicate. Minimal of inhibitory concentration (MIC) between the antibacterial effects of Berberis vulgaris (B. vulgaris) and F. vulgare Mill extracts individually and combined with nisin and sodium diacetate showed a significant difference against Yersinia enterocolitica. Among the studied factors, nisin had the most antibacterial effect and followed by the extract of B. vulgaris and F. vulgare Mill respectively. The antibacterial effect of sodium diacetate against Y. enterocolitica was the lowest. These results were almost consistent with those achieved by disk diffusion method. Our results indicated that MIC of sodium diacetate and nisin combined with the extracts of B. vulgaris and F. vulgare mill had a remarkable decrease compared to each one's effect individually (P<0.05). Results were similar about minimal bactericidal concentration (MBC), which showed simultaneous usage of sodium diacetate with these extracts can decrease the use of sodium diacetate a lonely and its complications. Moreover, combined consumption of extracts with nisin decreases the use of the extracts and the resultant organoleptic changes.