بررسي اثر هم‌افزايي عصاره ترخون(Artemisia dracunculus) و آسپيرين (ASA) بر روي فعاليت پلاكتي

The Synergic Effect of Artemisia Dracunculus (Tarragon) Extract and Aspirin (ASA) on Platelet Function

ﻣﻘﺪﻣﻪ: ﻓﻌﺎﻟﯿﺖ اﻧﻌﻘﺎدي ﭘﻼﮐﺖﻫﺎ در ﺷﮑﻞﮔﯿﺮي ﺗﺮوﻣﺒﻮز، ﭘﻼك آﺗﺮواﺳﮑﻠﺮوز و ﺑﺮوز ﺑﯿﻤﺎريﻫﺎي ﻗﻠﺒﯽ- ﻋﺮوﻗﯽ ﻧﻘﺶ وﯾﮋهاي را از ﺧﻮد ﻧﺸﺎن داده اﺳﺖ. ﺑﺮاي اﯾﻦ ﻣﻨﻈﻮر از ﻣﻬﺎرﮐﻨﻨﺪهﻫﺎي ﭘﻼﮐﺘﯽ ﺑﺮاي درﻣﺎن و ﺟﻠﻮﮔﯿﺮي از ﻋﺎرﺿﻪ ﻗﻠﺒﯽ اﺳﺘﻔﺎده ﻣﯽﺷﻮد ﮐﻪ ﭘﺮﻣﺼﺮف ﺗﺮﯾﻦ آﻧﻬﺎ آﺳﭙﯿﺮﯾﻦ ﻣﯽﺑﺎﺷﺪ. اﻣﺮوزه اﺳﺘﻔﺎده از داروﻫﺎي ﮔﯿﺎﻫﯽ ﻣﻮردﻧﻈﺮ ﻣﺘﺨﺼﺼﯿﻦ ﻃﺐ ﺳﻨﺘﯽ ﻣﯽﺑﺎﺷﺪ. ﻫﺪف: ﻫﺪف از اﯾﻦ ﻣﻄﺎﻟﻌﻪ، ﺗﻌﯿﯿﻦ ﻣﯿﺰان ﻓﻌﺎﻟﯿﺖ ﭘﻼﮐﺖﻫﺎ در ﻣﻮاﺟﻪ ﺑﺎ ﻋﺼﺎره ﮔﯿﺎه )ﺗﺮﺧﻮن( Artemisia dracunculus در ﮐﻨﺎر داروي ﻣﻬﺎرﮔﺮ ﭘﻼﮐﺘﯽ آﺳﭙﯿﺮﯾﻦ ﻣﯽﺑﺎﺷﺪ. روش ﺑﺮرﺳﯽ: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﭘﻼﮐﺖﻫﺎي اﻓﺮاد ﺳﺎﻟﻢ ﭘﺲ از آﻣﺎدهﺳﺎزي ﺑﺮاي ﻣﺪت زﻣﺎن 60 دﻗﯿﻘﻪ ﺑﺎ ﻏﻠﻈﺖﻫﺎي )µg/ml 125-1000( ﻋﺼﺎره ﻣﺘﺎﻧﻮﻟﯽ ﮔﯿﺎه ﺗَﺮﺧﻮن و ﻏﻠﻈﺖﻫﺎي )Acetyl Salisic (ASA) Acid] (0.31-1.26 µg/ml[، ﻫﺮ ﮐﺪام ﺑﻪ ﺗﻨﻬﺎﯾﯽ و ﻫﺮ دو ﻋﺼﺎره و دارو ﺑﺎ ﻫﻢ ﻣﻮاﺟﻪ ﺷﺪﻧﺪ، ﺳﭙﺲ آزﻣﺎﯾﺶﻫﺎي ﻓﻌﺎﻟﯿﺖ ﭘﻼﮐﺘﯽ ﮐﻪ ﺷﺎﻣﻞ: ﭼﺴﺒﻨﺪﮔﯽ ﭘﻼﮐﺘﯽ، اﮔﺮﯾﮕﻮﻣﺘﺮي و آزادﺳﺎزي ﭘﺮوﺗﺌﯿﻦ ﺑﺮ روي آﻧﻬﺎ اﻧﺠﺎم ﺷﺪ. ﻧﺘﺎﯾﺞ: ﻧﺘﺎﯾﺞ ﺑﻪ دﺳﺖ آﻣﺪه ﻧﺸﺎن ﻣﯽدﻫﺪ ﮐﻪ روﯾﺎروﯾﯽ ﭘﻼﮐﺖﻫﺎي ﻃﺒﯿﻌﯽ ﺑﺎ ﻋﺼﺎره ﺗﺮﺧﻮن ﺗﻮاﻧﺴﺘﻪ اﺳﺖ ﻓﻌﺎﻟﯿﺖ ﭘﻼﮐﺖﻫﺎ را در ﻫﺮ ﺳﻪ ﻣﺮﺣﻠﻪ ﺗﺠﻤﻊ، ﭼﺴﺒﻨﺪﮔﯽ و رﻫﺎﺳﺎزي ﭘﻼﮐﺘﯽ ﺑﻪ ﻃﻮر ﭼﺸﻤﮕﯿﺮي، در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﮐﻨﺘﺮل ﮐﺎﻫﺶ دﻫﺪ. ﻫﻤﭽﻨﯿﻦ، در اداﻣﻪ اﯾﻦ ﻋﺼﺎره ﺗﻮاﻧﺴﺖ ﺑﻪ ﻧﺤﻮ ﻗﺎﺑﻞﺗﻮﺟﻬﯽ )0/001 ≤P ﻓﻌﺎﻟﯿﺖ ﻫﻢاﻓﺰاﯾﯽ ﺿﺪﭘﻼﮐﺘﯽASA ﺑﺮ روي ﭘﻼﮐﺖﻫﺎي ﻃﺒﯿﻌﯽ را ﻧﯿﺰ ﺗﻘﻮﯾﺖ ﻧﻤﺎﯾﺪ. ﻧﺘﯿﺠﻪﮔﯿﺮي: ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ اﺳﺘﻔﺎده ﻫﻤﺰﻣﺎن ﻋﺼﺎره ﮔﯿﺎه ﺗَﺮﺧﻮن و ASA ﻣﯽﺗﻮاﻧﺪ ﺑﻪ ﻋﻨﻮان ﯾﮏ ﺗﺮﮐﯿﺐ ﻣﺆﺛﺮ ﺳﺒﺐ اﻟﻘﺎي ﻓﻌﺎﻟﯿﺖ ﺿﺪﭘﻼﮐﺘﯽ ﺷﻮد، ﻟﺬا اﺳﺘﻔﺎده از اﯾﻦ ﻋﺼﺎره در درﻣﺎن ﺑﯿﻤﺎريﻫﺎي ﻗﻠﺒﯽ- ﻋﺮوﻗﯽ و ﺗﺮوﻣﺒﻮﺗﯿﮏ ﭘﯿﺸﻨﻬﺎد ﮐﺮد.

Background: Coagulation activity of platelets plays an important role in thrombosis formation, Atherosclerotic plaque establishment and cardiovascular disorders. ASA are using as an anti platelet activity drug for patients with Coronary Artery Disease (CAD). Using of plant extraction as a complementary medicine for these patients (CAD) is mostly recommended by traditional medicine specialist. Objective: In this study we investigated platelet activity after treatment with extract of Artemisia dracunculus (Tarragon) and it’s synergic with ASA treatment. Methods: After preparation of platelets from healthy volunteers, platelets confronted with desired concentrations (125-1000 µg/ml) of methanol extract of Artemisia dracunculus (tarragon) and (0.31-1.26 µg/ml) of (ASA) alone and in combination. The platelet function such as adhesion, aggregation and protein release were assayed on treated platelets and controls. Results: Our results demonstrated that Tarragon extract significantly effective on adhesion, aggregation and protein release in comparison with healthy volunteers platelets (P≤0.001). The Tarragon extract also raised the anti-platelet activity of ASA on these platelets. Conclusion: Tarragon extract, either as single agent or combination with ASA, has been able to induce anti-platelet activity. Therefore, it would be suggested to use of the tarragon extract with or without common treatments, i.e. ASA, in treatments of cardiovascular disorders and patients with deep vein thrombosis.