ﺳﻨﺠﺶ ﻏﻠﻈﺖ ﻫﺎي اﯾﻨﺘﺮﻟﻮﮐﯿﻦ ﻫﺎي5 ،3 و 6 در ﻣﺎﯾﻊ ﻓﻮﻟﯿﮑﻮﻟﯽ و ارزش ﺗﺸﺨﯿﺼﯽ آنﻫﺎ در زﻧﺎن ﻧﺎﺑﺎرور ﺑﺎ ﻋﻠﺖ ﻧﺎﺷﻨﺎﺧﺘﻪ

Assessment Interleukins 3, 5 and 6 Concentration in Follicular Fluid and Their Diagnostic Value in Patients with Unexplained Infertility

زﻣﯿﻨﻪ و ﻫﺪف: ﺳﺎﯾﺘﻮﮐﺎﯾﻦ ﻫﺎي ﺳﯿﺴﺘﻢ اﯾﻤﻨﯽ در ﻓﺮآﯾﻨﺪ ﻫﺎي ﻣﺘﻌﺪد ﻓﯿﺰﯾﻮﻟﻮژﯾﮑﯽ در ﺳﯿﺴﺘﻢ ﺗﻮﻟﯿﺪ ﻣﺜﻞ ﻓﻌﺎﻟﯿﺖ دارﻧﺪ. ﻫﻢ ﭼﻨﯿﻦ ﺑﯿﻦ ﺳﯿﺴﺘﻢ اﯾﻤﻨﯽ و ﺗﻮﻟﯿﺪ ﻣﺜﻞ ﯾﮏ راﺑﻄﻪ دو ﻃﺮﻓﻪ وﺟﻮد دارد. ﻫﺪف اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﻣﻘﻄﻌﯽ ﺑﺮرﺳﯽ ﻏﻠﻈﺖﻫﺎي اﯾﻨﺘﺮﻟﻮﮐﯿﻦﻫﺎي3 ،5 و 6 در ﻣﺎﯾﻊ ﻓﻮﻟﯿﮑﻮﻟﯽ در اﻓﺮاد داراي ﻧﺎﺑﺎروري ﺑﺎ ﻋﻠﺖ ﻧﺎﺷﻨﺎﺧﺘﻪ و ﻣﻘﺎﯾﺴﻪ آنﻫﺎ ﺑﺎ اﻓﺮاد ﺳﺎﻟﻢ در ﻃﻮل درﻣﺎن ﺑـﺎ ﻟﻘـﺎح آزﻣﺎﯾﺸـﮕﺎﻫﯽ و ﻓـﺎﮐﺘﻮر ﻫـﺎي ﺑﺎﻟﯿﻨﯽ آنﻫﺎ ﺑﻮد. روش ﮐﺎر: ﺷﺮوع ﺗﺤﺮﯾﮏ ﺗﺨﻤﮏﮔﺬاري ﺑﺎ داروﻫﺎي ﻣﺤﺮك ﺗﺨﻤﮏﮔﺬاري ﺗﺤﺖ ﭘﺮوﺗﮑﻞ آﻧﺘﺎﮔﻮﻧﯿﺴﺖ و آﮔﻮﻧﯿﺴـﺖ در 60 ﺑﯿﻤـﺎر ﻧﺎﺑﺎرور ﺑﺎ ﻋﻠﺖ ﻧﺎﺷﻨﺎﺧﺘﻪ و 40 ﺧﺎﻧﻢ ﺳﺎﻟﻢ آﻏﺎز ﮔﺮدﯾﺪ. ﺳﭙﺲ ﻣﺎﯾﻊ ﻓﻮﻟﯿﮑﻮﻟﯽ آنﻫﺎ ﺟﺪا ﺷﺪه و ﻏﻠﻈﺖ ﻓﻮﻟﯿﮑﻮﻟﯽ اﯾﻨﺘﺮﻟﻮﮐﯿﻦ ﻫـﺎي 5 ،3 و 6 ﺑﺎ روش اﻻﯾﺰا اﻧﺪازهﮔﯿﺮي ﺷﺪ و ﻫﻢﭼﻨﯿﻦ ارزش ﺗﺸﺨﯿﺼﯽ اﯾﻦ3 اﯾﻨﺘﺮﻟﻮﮐﯿﻦ ﺑﺎ اﺳﺘﻔﺎده از ﺳﻄﺢ زﯾﺮ ﻣﻨﺤﻨﯽ)Roc curve( ﻣـﻮرد ﺑﺮرﺳـﯽ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﯾﺎﻓﺘﻪﻫﺎ: از ﻓﺎﮐﺘﻮرﻫﺎي ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﺿﺨﺎﻣﺖ آﻧﺪوﻣﺘﺮ و ﺳﻄﺢ ﺗﺴﺘﻮﺳﺘﺮون ﺑﯿﻦ دو ﮔﺮوه ﻣﻌﻨﯽدار ﺷﺪﻧﺪ)0/0001

Inroduction & Objective: Our aim a cross-sectional study was to compare the follicular fluid level of interleukins 3, 5, and 6 and clinical factors in patients with unexplained infertility with healthy subjects in the outcome of In vitro fertilization (IVF). Material and Methods: Initiation of ovulation stimulating with ovulation stimulating drugs in different protocols started in 60 infertile patients with unexplained infertility and 40 fertile women. Subjects underwent antagonist and agonist protocol for ovarian stimulation.Then their follicular fluid was separated and the follicular concentration of interleukins 3, 5 and 6 determined by Enzyme-linked immunosorbent assay (ELISA) method. The diagnostic value of these 3 interleukins was also evaluated using the Roc curve. Results: Among the clinical factors, the endometrial thickness and testosterone levels significantly between the two groups (P <0.0001and P = 0.002), that the mean endometrial thickness was lower and the mean testosterone level was higher than the control group. So, hirsutism and hyperandrogenism significantly correlated between the patient and control groups (P = 0.046 and P = 0.046). Interleukin-3, 5, and 6 concentrations decreased in the patient, that the only reduction in IL-5 concentration significantly in the case group compared to the healthy group (P <0.0001). The diagnostic accuracy of IL-5 in the FF wasmoderate with the area under the curve (AUC) of 0.71. Conclusion: There is a significant difference in the follicular fluid interleukin-5 concentration between infertile and fertile women. This cytokine may be involved in the pathophysiology of unexplained infertility.