بررﺳﯽ اﺛﺮ ﭘﺮوﺑﯿﻮﺗﯿﮏ ﮐﻠﻮﺳﺘﺎت)Bacillussubtilis( ﺑﺮ روي ﺑﺮﺧﯽ از ﺷﺎﺧﺺﻫﺎي رﺷﺪ، ﺧﻮﻧﯽ و ﺑﺎﻓﺖ روده ﻣﺎﻫﯽ ﻗﺰلآﻻي رﻧﮕﯿﻦﮐﻤﺎن)Oncorhynchusmykiss(

Evaluation of the effect of Clustat probiotic (Bacillus subtilis) on some growth and hematological factor as well as intestine tissue of the rainbow trout (Oncorhynchus mykiss)

زﻣﯿﻨﻪ و ﻫﺪف: اﻣﺮوزه در ﺻﻨﻌﺖ آﺑﺰي ﭘﺮوري، اﺳـﺘﻔﺎده از ﭘﺮوﺑﯿﻮﺗﯿـﮏﻫﺎﺑـﻪ ﻋﻨـﻮان ﺟـﺎﯾﮕﺰﯾﻦ ﻣﻨﺎﺳـﺐ آﻧﺘـﯽ ﺑﯿﻮﺗﯿـﮏﻫـﺎاﻓﺰاﯾﺶ ﭼﺸﻤﮕﯿﺮي داﺷﺘﻪ اﺳﺖ.اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﻪ ﺑﺮرﺳﯽ اﺛﺮ رژﯾﻢ ﺧﻮراﮐﯽ ﺣﺎوي ﭘﺮوﺑﯿﻮﺗﯿﮏ ﺗﺠﺎريKEMIN®)B. subtilis(ﺑﺮ ﺷﺎﺧﺺﻫﺎي رﺷـﺪ، ﺧﻮﻧﯽ و ﻣﻮرﻓﻮﻟﻮژي ﺑﺎﻓﺖ روده در ﻣﺎﻫﯽ ﻗﺰلآﻻي رﻧﮕﯿﻦﮐﻤﺎن )Oncorhynchos mykiss( ﭘﺮداﺧﺘﻪ اﺳﺖ. روش ﮐﺎر: در اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﻌﺪاد 135 ﻋﺪد ﻣﺎﻫﯽ ﺑﺎ وزن 35/68±2/5 ﮔﺮم ﺑﺎ رژﯾﻢ ﻏﺬاﯾﯽ ﺣﺎوي ﭘﺮوﺑﯿﻮﺗﯿﮏ ﺑﺎﺳـﯿﻠﻮس ﺑـﻪ ﻣـﺪت50 روز ﺗﻐﺬﯾﻪ ﺷﺪﻧﺪ. ﺗﯿﻤﺎرﻫﺎ ﺷﺎﻣﻞ ﺳﻪ ﺟﯿﺮه آزﻣﺎﯾﺸﯽ ﺣﺎوي ﮔﺮوه ﺷﺎﻫﺪ)ﺑﺪون ﭘﺮوﺑﯿﻮﺗﯿﮏ(، 1 و 2 درﺻﺪ ﭘﺮوﺑﯿﻮﺗﯿﮏ ﺑﻮد. در ﭘﺎﯾـﺎن دوره از ﻣﺎﻫﯽ ﻫﺎ ﺑﻪ ﺻﻮرت ﺗﺼﺎدﻓﯽ ﻧﻤﻮﻧﻪ ﺑﺮداري ﺷﺪ. ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﯽ ﺷﺎﺧﺺﻫـﺎي ﺧﻮﻧﯽ)ﺗﻌـﺪاد ﮔﻠﺒـﻮلﻫـﺎي ﺳـﻔﯿﺪ و ﻗﺮﻣـﺰ، ﻫﻤﺎﺗﻮﮐﺮﯾـﺖ، ﻫﻤﻮﮔﻠﻮﺑﯿﻦ و ﺷﻤﺎرش اﻓﺘﺮاﻗﯽ ﮔﻠﺒﻮلﻫﺎي ﺳﻔﯿﺪ(ﺧﻮن ﮔﯿﺮي از ﺳﯿﺎﻫﺮگ دﻣﯽ اﻧﺠﺎم و ﻃﻮل و ﻋـﺮض ﭘﺮزﻫـﺎي روده، اﻧـﺪازه و ﺗـﺮاﮐﻢ ﺳﻠﻮلﻫﺎي ﺟﺎﻣﯽ ﺷﮑﻞ در ﺑﺮرﺳﯽ ﺑﺎﻓﺖ ﺷﻨﺎﺳﯽ اﻧﺪازهﮔﯿﺮي ﮔﺮدﯾﺪ. ﻫﻢﭼﻨﯿﻦ ﺷﺎﺧﺺﻫﺎي رﺷﺪ ﻧﯿﺰ ﻣﻮرد ﺳﻨﺠﺶ ﻗﺮار ﮔﺮﻓﺖ. ﯾﺎﻓﺘــﻪ ﻫــﺎ: در ﭘﺎﯾــﺎن دوره ﺗﻐﺬﯾــﻪ، ﻋﻤﻠﮑــﺮد رﺷــﺪ ﻧﺴــﺒﺖ در ﺗﯿﻤﺎرﻫــﺎي ﭘﺮوﺑﯿﻮﺗﯿــﮏ ﻧﺴــﺒﺖ ﺑــﻪ ﺗﯿﻤــﺎر ﺷــﺎﻫﺪ اﻓــﺰاﯾﺶ ﻣﻌﻨــﯽداري داﺷﺖ)0/05 p(. ﻧﺘﯿﺠﻪ ﮔﯿﺮي: ﻧﺘﺎﯾﺞ ﺣﺎﺻﻞ از اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﻧﺸﺎن داد ﮐﻪ اﺳﺘﻔﺎده ﺧـﻮراﮐﯽ از ﭘﺮوﺑﯿﻮﺗﯿـﮏ B. subtilis اﺛـﺮات ﺳـﻮدﻣﻨﺪ و ﻣﺜﺒـﺖ ﺑـﺮ ﭘﺎراﻣﺘﺮﻫﺎي رﺷﺪ، ﺧﻮﻧﯽ و ﻫﻢﭼﻨﯿﻦ ﺑﺎﻓﺖ روده ﻣﺎﻫﯽ ﻗﺰلآﻻي رﻧﮕﯿﻦﮐﻤﺎن داﺷﺖ.

Inroduction & Objective:At present, the use of probiotics as goodalternative for antibiotics is greatlyincreasing in aquaculture industry. The objectives of this study was to evaluate the effect of dietary supplementation of B. subtilis probiotic (KEMIN ®) on growth and hematological parameters and also the morphology of intestine of rain bow trout (Oncorhynchus mykiss). Material and Method:In this study 135 fish with an average initial weight of 35.68 ± 2.5 g were fed diets supplemented with of probiotic after 50 days. Experimental treatments included control without probiotic (control group), 1 and 2% probiotic levels.Fish were randomly sampled at the end of experimental period. The blood was extracted from caudal vein of samples for measuringblood parameters (total red and white cells, hemoglobin, differential blood cell count).Intestinal morphology including height and width of villi, density and area of goblet cells were quantified for histological examination. Growth parameters was also determined. Results: At the end of the feeding trial, fish fed with probiotic supplemented diets had significantly higher growth performance than control group (p < 0.05). In addition, fish fed with probiotic diets had significantly higher red blood cell, neutrophils and height of villi compared to the control group (p < 0.05). No significant difference of some hematological parameters (such as white blood cell, hematocrit, hemoglobin, neutrophils and monocyte) and intestinal morphology (width of villi, density and area of goblet cells) was observed in all groups (p> 0.05). Conclusion: The results of this study showed that the oral administration of B. subtilis possessed the beneficial and positive effects on growth and hematological factors as well as intestine tissue of rain bow trout.