عنوان مقاله :
Effective Induction of Hairy Roots in Persian Poppy (Papaver bracteatum Lindl.) Using Sonication Method
پديد آورندگان :
Qavami ، N Ferdowsi University of Mashhad - Faculty of Agriculture - Department of Horticultural Sciences , Azizi ، M Ferdowsi University of Mashhad - Faculty of Agriculture - Department of Horticultural Sciences , Yazdian ، F University of Tehran - Faculty of New Sciences and Technologies - Department of Life Science Engineering , Qaderi ، A Academic Center for Education, Culture and Research (ACECR) - Institute of Medicinal Plants - Medicinal Plants Research Center , Nemati ، H Ferdowsi University of Mashhad - Faculty of Agriculture - Department of Horticultural Sciences
كليدواژه :
Papaver bracteatum Lindl. Hairy root , Sonication , assisted Agrobacterium , mediated transformation , Thebaine , Thebeine
چكيده فارسي :
Background: Papaver bracteatum Lindl. Commonly known as Iranian poppy is an important medicinal plant due to the presence of benzylisoquinoline alkaloids. Objective: To evaluate the hairy root culture as a novel method for thebaine production. Methods: To optimize the hairy root culture of P. bracteatum, five strains of Agrobacterium rhizogenes (ATCC15834, C318, A13, 9453 and A4) were used by means of SonicationAssisted Agrobacteriummediated Transformation (SAAT). Results: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41 deg;C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers. Conclusion: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate (28.5 %) was observed 4 weeks after inoculation, once the explants were subjected to sonication (45 s) following a heat treatment of 41 deg;C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers.
عنوان نشريه :
گياهان دارويي
عنوان نشريه :
گياهان دارويي
