كليدواژه :
چاي , جوانه خواب , جوانه فعال , فعاليت آنزيم اكسيداني
چكيده فارسي :
عملكرد و كيفيت عصاره برگي گياه چاي (Camellia sinensis L.) به عنوان يك نوشيدني سالم در سراسر جهان،بستگي به رشد رويشي شاخساره و خواب جوانه دارد. خواب جوانه در مرحله برداشت برگ باعث افزايش فاصله برداشت برگ مي شود و عملكرد را كاهش مي دهد. در اين مطالعه تغييرات فعاليت آنزيم هاي آنتي اكسيداتيو در جوانه هاي فعال و خواب گياه چاي در دو رقم كلون 100 و هيبريد بررسي شد. نتايج نشان داد تغييرات معني داري در فعاليت آنزيم هاي مورد بررسي وجود داشت كه حاكي از وجود تنوع ژنتيكي بين آنها بود. فعاليت آنزيم ها از جمله آسكوربات پراكسيداز، كاتالاز و پراكسيداز در جوانه خواب بالاتر از جوانه فعال بود، اما فعاليت آنزيم پليفنل اكسيداز در جوانه فعال بيشتر از جوانه خواب بود. با توجه به نتايح بدست آمده به نظر م يرسد تغييرات بيوشيميايي در ايجاد جوانه خواب و جوانه فعال نقش داشته و از اين تغييرات ميتوان به‌عنوان نشانگر بيوشيميايي در برآورد تشخيص عملكرد ارقام چاي و كاهش دوره خواب جوانه بهره جست.
چكيده لاتين :
The yield and quality of tea depend on the shoot growth and bud dormancy. Tea (Camellia sinensis) is one of the natural sources of bioactive compounds, such as caffeine, catechins, and antioxidants, which is consumed as a “health drink” due to its beneficial medicinal properties. The bud dormancy increases the distance of leaf plucking and reduces the yield of tea. In this study, activities of antioxidant enzymes were assessed in banjhi and active buds in two cultivars, clone 100 and hybrid. The results showed that there were significant differences in antioxidant enzyme activity, indicating genetic diversity between them. The activities of enzymes such as ascorbate peroxidase, catalase and peroxidase in the banjhi buds were higher than the active buds, however, in banjhi buds, the peroxidase activity was higher than the active budsThe yield and quality of tea, which is consumed as a “health drink”, depend on the shoot growth and bud dormancy. The bud dormancy increases the distance of leaf plucking and reduces the yield of tea. In this study, activities of antioxidant enzymes were assessed in banjhi and active buds in two cultivars, clone 100 and hybrid. The results showed that there were significant differences in antioxidant enzyme activity, indicating genetic diversity between them. The activities of enzymes such as ascorbate peroxidase, catalase and peroxidase in the banjhi buds were higher than the active buds, however, in banjhi buds, the peroxidase activity was higher than the active buds.
According to the results, it seems that biochemical changes are involved in the development of banjhi bud and active bud and these changes can be used as a biochemical marker not only in detecting the yield of tea cultivars but also in reducing the dormant periods.
