ويژگي يابي و بررسي اثرات ضد ميكروبي نانوذرات اكسيد روي/نقره سنتز شده با عصاره دانه گياه خار مريم (Silybum marianum): يك گزارش كوتاه

Characterization and Evaluation of Antimicrobial Effects of ZnO/Ag Nanoparticles Synthesized by Milk Thistle Seed Extract (Silybum marianum): A Short Report

زﻣﯿﻨﻪ و ﻫﺪف: اﺳﺘﻔﺎده از ﻓﻨﺎوري ﻧﺎﻧﻮ در زﻣﯿﻨﻪ ﻫﺎي ﻣﺨﺘﻠﻒ ﺑﻪ وﯾﮋه در زﻣﯿﻨﻪ ﺑﻬﺪاﺷﺘﯽ و داروﯾﯽ ﺑﻪ ﺳﺮﻋﺖ در ﺣﺎل ﮔﺴﺘﺮش ﻣﯽ ﺑﺎﺷﺪ. ﻫﺪف از اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺗﻮﻟﯿﺪ ﻧﺎﻧﻮذرات اﮐﺴﯿﺪ روي/ﻧﻘﺮه زﯾﺴﺘﯽ ﺑﺎ اﺳﺘﻔﺎده از ﻋﺼﺎره داﻧﻪ ﺧﺎرﻣﺮﯾﻢ و ﺗﻌﯿﯿﻦ اﺛﺮات ﺿﺪ ﻣﯿﮑﺮوﺑﯽ آنﻫﺎ ﻣﯽﺑﺎﺷﺪ. ﻣﻮاد و روشﻫﺎ: در اﯾﻦ ﭘﮋوﻫﺶ آزﻣﺎﯾﺸﮕﺎﻫﯽ ﻧﺎﻧﻮذرات زﯾﺴﺘﯽ ﺑﺎ اﺳﺘﻔﺎده از ﻋﺼﺎره آﺑﯽ داﻧﻪ ﺧﺎر ﻣﺮﯾﻢ و ﻧﺎﻧﻮذرات اﮐﺴﯿﺪ روي ﺑﻪ روش ﺷﯿﻤﯿﺎﯾﯽ ﺳﻨﺘﺰ ﺷﺪﻧﺪ، ﭘﺲ از ﺑﺮرﺳﯽ ﺳﺎﺧﺘﺎر، اﺛﺮات ﺿﺪ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ اﯾﻦ ﻧﺎﻧﻮﻣﻮاد در ﻏﻠﻈﺖ ﻫﺎي0/8، 0/4، 0/2، 0/1 ، 0/05 و ﺻﻔﺮ )ﺷﺎﻫﺪ( ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﻣﯿﻠﯽﻟﯿﺘﺮ ﺑﺮ ﺑﺎﮐﺘﺮي ﻫﺎي Escherichia coli و Staphylococcus aureus ﻣﻄﺎﻟﻌﻪ ﮔﺮدﯾﺪ. ﯾﺎﻓﺘﻪﻫﺎ: اﻧﺪازه ﻧﺎﻧﻮذرات اﮐﺴﯿﺪروي/ﻧﻘﺮه ﺳﻨﺘﺰ ﺷﺪه ﺑﻪ روش زﯾﺴﺘﯽ، 17/5 ﻧﺎﻧﻮﻣﺘﺮ ﺗﻌﯿﯿﻦ ﺷﺪ ﮐﻪ ﻧﺴﺒﺖ ﺑﻪ ﻧﺎﻧﻮذرات ﺷﯿﻤﯿﺎﯾﯽ )22 ﻧﺎﻧﻮﻣﺘﺮ( ﮐﻮﭼﮏﺗﺮ ﺑﻮدﻧﺪ ﮐﻪ اﯾﻦ اﻧﺪازه ﮐﻮﭼﮏ در ﮐﺎرﺑﺮد زﯾﺴﺘﯽ آن ﻣﯽ ﺗﻮاﻧﺪ ﺗﺄﺛﯿﺮﮔﺬار ﺑﺎﺷﺪ. ﻫﻢﭼﻨﯿﻦ ﺣﻀﻮر ﺗﺮﮐﯿﺒﺎت آﻟﯽ ﮔﯿﺎﻫﯽ در ﺳﺎﺧﺘﺎرﻧﺎﻧﻮذرات زﯾﺴﺘﯽ ﺗﺄﯾﯿﺪ ﺷﺪﻧﺪ. ﻫﺮ دو ﻧﻮع ﻧﺎﻧﻮذره اﺛﺮات ﺿﺪ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ واﺑﺴﺘﻪ ﺑﻪ دوز داﺷﺘﻨﺪ. ﺑﺎ اﯾﻨﮑﻪ ﺣﺴﺎﺳﯿﺖ ﺑﺎﮐﺘﺮي ﻫﺎ ﻧﺴﺒﺖ ﺑﻪ ﻧﺎﻧﻮذرات زﯾﺴﺘﯽ ﺑﯿﺶﺗﺮ ﺑﻮد وﻟﯽ اﯾﻦ اﺧﺘﻼف از ﻧﻈﺮ آﻣﺎري ﻓﻘﻂ در ﻏﻠﻈﺖ 0/8 ﻣﯿﻠﯽ ﮔﺮم/ﻣﯿﻠﯽ ﻟﯿﺘﺮ ﻣﻌﻨﯽدار ﺑﻮد )0/05

The use of nanotechnology is rapidly expanding in various fields, especially in the health and pharmaceutical fields. The purpose of this study was to produce biological Zinc Oxide (ZnO) and silver (Ag) nanoparticles (NPs) using extract of milk thistle seeds and to determine their antibacterial properties. Materials and Methods: In this laboratory study, biological NPs were synthesized using aqueous extract of milk thistle, and ZnO NPs were chemically synthesized as well. After investigating the structural properties, the antibacterial effects of the nanomaterials on Escherichia coli and Staphylococcus aureus were studied at the concentrations of 0.8, 0.4, 0.2, 0.1, 0.05 and 0 (control) mg/ml. Results: The size of the biologically synthesized ZnO/Ag NPs was determined (17.5 nm), which was smaller than the chemical NPs (22 nm). Due to smaller size, it could have more bioactivity. Furthermore, the presence of organic compounds in the structure of NPs was confirmed. Both types of NPs showed dose-dependent antibacterial effects. Despite high sensitivity of bacteria to the biological NPs, this difference was statistically significant only at theconcentration of 0.8 mg/ml (p<0.05). Conclusion: The findings showed that biological NPs were more effective on the bacterial growth. This could be due to their smaller size and presence of active plant compounds in their structure