بررسي اثر عصاره هيدروالكلي گياه برازمبل Perovskia abrotanoides بر دانسيته نوروني موش‌هاي صحرايي تحت استرس مزمن بي‌حركتي

The Effect of Hydroalcoholic Extract of Perovskia Abrotanoides on Neuronal Density of Rats under Chronic Immobility Stress

مقدمه تعداد نورون هاي مناطق هيپوكامپ مي تواند تاثير مستقيمي بر عملكرد آن داشته باشد . گياه برازمبل از تيره نعناعيان داراي خواص آنتي اكسيداني شايد است. هدف بررسي اثرات استرس و عصاره برازمبل بر دانسيته نوروني CA1,CA2 در موش صحرايي نر بود. روش بررسي در اين مطالعه تجربي موش هاي صحرايي نژاد ويستار به 5 گروه 6 تايي شامل كنترل، كنترل منفي تحت استرس ، تجربي (شامل: استرس و دريافت كننده عصاره برازمبل با دوزهاي mL 50، mL 75 و mL 100) تقسيم شدند. گروه استرس مدت 21 روز داخل محدود كننده رت مدت 2 ساعت قرار گرفتند. گروه هاي دريافت كننده عصاره نيز به مدت 21 روز، به روش گاواژ عصاره را دريافت داخل محدود كننده قرار گرفتند. پس از خارج كردن مغز با عمل پرفيوژن، هيپوكامپ برش گيري و رنگ آميزي شد ، دانسيته نوروني به روش دايسكتور و متداستريولوژي محاسبه شد. نتايج توسط نرم افزار Minitab 16 با آزمون آماري t-test و ANOVA در سطح معناداري (0/05

Introduction: The number of neurons in the hippocampus can have a direct effect on its function. The plant Brazembel from the mint family may have antioxidant properties. The aim of the present study was to investigate the effects of stress and Brazmble extract on neuronal density of CA1, CA2 in male rats. Methods: In this experimental study, Wistar rats were divided into 6 groups (n=5), including control, and negative control under stress, also experimental group,including stress and get Brazmbl doses of the extract mL 50, mL 75 and mL 100. Limiting stress for 21 days, the rats were exposed in 2 hours. Groups receiving the extract for 21 days, to get inside limiting extracts were gavaged. After removal of the brain by perfusion, the hippocampus was excised and stained, and the neuronal density was calculated by dissector and metasteriology. The results were analyzed by Minitab 16 software with t-test and ANOVA at a significant level (P <0.05). Results: Statistical analysis of the data showed that the mean neuronal density in the stress group compared to the control in CA1 and CA2 had a significant decrease (p <0.01). The mean neuronal density of CA1 region between the stress group (29 ± 3) and hydroalcoholic treatment at 50 mg / kg (25 ± 3) showed a significant increase. In addition, the mean neuronal density in the stress group with 75 mg / kg extract (27±3) and in the group treated with hydroalcoholic extract was 100 mg / kg (25 ± 3). Mean neuronal density of CA2 region between the stress (21 ± 3) and hydroalcoholic treatment at 50 mg / kg (28 ± 3) groups showed a significant increase (p <0.01). The mean neuronal density in the stress group with 75 mg / kg extract was 27 ± 3 and in the hydroalcoholic extract treatment group was 100 mg / kg (22 ± 3), which increased significantly compared to the stress group (p <0.01). Conclusion: The results of the current study show that the hydroalcoholic extract of plant Bromzebel probably increases the neuronal density of the brain hippocampus due to its polyphenolic substances such as flavonoids, which is due to its high antioxidant properties.