كليدواژه :
Great tit , Karyotype , Chromosomal Spread , Cytogenetic , Noor forest park , IRAN , چرخ ريسك بزرگ , گستره كروموزومي , كاريوتايپ , پارك جنگلي نور
چكيده لاتين :
Cytogenetical characters of great tit (Parus major), were studied in Noor forest park (Mazandaran-Iran). After in vivo colchicine treatment, liver, bone marrow of femur and tibia tissues were used and karyological parameters such as major and minor arms, centromeric index, arm ratio, relative length, total length and variation range of chromosomes length were determined on chromosomal slides as well as karyogram and idiogram. Final results show that maximum metaphase index were belong to bone marrow of tibia samples. Chromosomal number varied between 70-80, consist of one pair metacentric, three pairs submetacentric and six pairs acrocentric which were constant and visible on all spreads and the rest were variable microchromosomes. Sex determination mechanism were defined as ZW; but in none of the females studied could a W chromosome be identified, probably it placed in microchromosomes set, which will needed further studies. Karyological parameters show that centromeric index, arm ratio, relative length and variation range of chromosomes length were between 20-50, 1-4.7, 4-24 and 0.83-5 respectively and total length and NF were 20.82 and 26. karyotypic formula were determined as 2n=2m+5sm+12a+(56±5 microchromosomes).
