اثر ضداضطرابي آلفاپينن در مقايسه با ديازپام در موش صحرايي نر بالغ

Anti-anxiety effect of Alpha-pinene in comparison with Diazepam in adult male rats

ﺳﺎﺑﻘﻪ و ﻫﺪف: ﺑﺎﺗﻮﺟﻪ ﺑﻪ ﻋﻮارض ﺟﺎﻧﺒﻲ داروﻫﺎي ﺷﻴﻤﻴﺎﻳﻲ ﺿﺪاﺿﻄﺮاب، در اﻳﻦ ﭘﮋوﻫﺶ ﺗﺄﺛﻴﺮ آﻟﻔﺎﭘﻴﻨﻦ ﺑﺮ رﻓﺘﺎرﻫﺎي اﺿﻄﺮاﺑﻲ در ﻣﻘﺎﻳﺴﻪ ﺑﺎ دﻳﺎزﭘﺎم در ﻣﻮش ﺻﺤﺮاﻳﻲ ﻣﻮرد ﺑﺮرﺳﻲ ﻗﺮار ﮔﺮﻓﺖ. ﻣﻮاد و روش ﻫﺎ: در اﻳﻦ ﻣﻄﺎﻟﻌﻪ 40 ﺳﺮ ﻣﻮش ﺻﺤﺮاﻳﻲ ﻧﺮ )ﻧﮋاد وﻳﺴﺘﺎر( در 5 ﮔﺮوه: ﻛﻨﺘﺮل، ﺷﺎﻫﺪ درﻳﺎﻓﺖ ﻛﻨﻨﺪه ﺣﻼل آﻟﻔﺎﭘﻴﻨﻦ(،درﻳﺎﻓﺖ ﻛﻨﻨﺪه دوز 2 و 4 ﻣﻴﻠﻲ ﮔﺮم ﻛﻴﻠﻮﮔﺮم آﻟﻔﺎﭘﻴﻨﻦ و ﮔﺮوه درﻳﺎﻓﺖ ﻛﻨﻨﺪه دوز 10 ﻣﻴﻠﻲ ﮔﺮم ﻛﻴﻠﻮﮔﺮم دﻳﺎزﭘﺎم ﺗﻘﺴﻴﻢ ﮔﺮدﻳﺪﻧﺪ. داروﻫﺎ ﺑﻪ ﻣﺪت دو ﻫﻔﺘﻪ ﺑﻪ ﺻﻮرت درون ﺻﻔﺎﻗﻲ ﺗﺠﻮﻳﺰ ﺷﺪﻧﺪ. در ﭘﺎﻳﺎن اﻳﻦ دوره، اﺿﻄﺮاب ﺑﺎ اﺳﺘﻔﺎده از ﻣﺪل ﻣﺎز ﺑﻌﻼوه اي ﺷﻜﻞ ﻣﺮﺗﻔﻊ ﻣﻮرد ﺳﻨﺠﺶ ﻗﺮار ﮔﺮﻓﺖ و ﻏﻠﻈﺖ ﻣﺎﻟﻮن دي آﻟﺪﺋﻴﺪ، ﺗﻴﻮل و ﮔﻠﻮﺗﺎﺗﻴﻮن ﭘﺮاﻛﺴﻴﺪاز در ﺑﺎﻓﺖ ﻫﻴﭙﻮﻛﺎﻣﭗ اﻧﺪازه ﮔﻴﺮي ﺷﺪ. ﻧﺘﺎﻳﺞ ﺑﺎ اﺳﺘﻔﺎده از آزﻣﻮن ﻫﺎي آﻣﺎري آﻧﺎﻟﻴﺰ وارﻳﺎﻧﺲ ﻳﻚ ﻃﺮﻓﻪ و آزﻣﻮن ﺗﻮﻛﻲ ﺗﺠﺰﻳﻪ و ﺗﺤﻠﻴﻞ ﺷﺪﻧﺪ. ﻧﺘﺎﻳﺞ: آﻟﻔﺎﭘﻴﻨﻦ در ﮔﺮوه ﻫﺎي درﻳﺎﻓﺖ ﻛﻨﻨﺪه دوزﻫﺎي 2 و 4 ﻣﻴﻠﻲ ﮔﺮم ﺑﺮ ﻛﻴﻠﻮﮔﺮم آﻟﻔﺎﭘﻴﻨﻦ ﺑﻪ ﻃﻮر ﻣﻌﻨﻲ داري ﻣﻨﺠﺮ ﺑﻪ اﻓﺰاﻳﺶ درﺻﺪ ورود ﺑﻪ ﺑﺎزوي ﺑﺎز )0/001

Considering the side effects of anti- anxiety drugs, this study investigated the effect of alpha-pinene on anxiety behaviors compared with Diazepam in rats. Materials and Methods In this study, 40 male Wistar rats were divided into 5 groups: control, sham (received alphapinene solvent), the groups received 2 and 4 mg/kg alphapinene, and the group that received diazepam 10 mg/kg. At the end of this period, anxiety was measured using elevated plus maze and concentrations of malondialdehyde, thiol and glutathione peroxidase, in hippocampal tissue. The results were analyzed using one-way ANOVA and Tukey tests. Results: In the groups receiving 2 and 4 mg/kg doses of alpha-pinene significantly increased the percentage of open arm entry (P<0.001) and percentage of time spent in the open arm (P<0.05) (P<0.05). Compared to the control and diazepam groups, administration of alphapinene significantly decreased MDA concentration (P<0.001) and significant increase in thiol (P<0.01) (P<0.05) and glutathione peroxidase enzyme activity was evident (P<0.001). Conclusion: Alpha-pinene showed a downward effect on anxiety responses in male rats, similar to anxiolytic effects of diazepam. Alpha-pinene may be potentiated by binding to the position of benzodiazepines in GABA A receptors and exerted its anti-anxiety effect with antioxidant properties.