تاثير ورزش تردميل و سلول هاي بنيادي مشتق از مغز استخوان پيش شرطي شده با دي متيل اگزاليل گليسين بر نوروتوكسيسيتي القاء شده توسط آميلوئيد بتا در موش هاي صحرائي نر بالغ

Effects of treadmill exercise and preconditioned bone marrow- derived mesenchymal stem cells transplantation on Aβ-induced neurotoxicity in male rats

ﻫﺪف: ﭘﯿﺶ ﺷﺮﻃﯽ ﮐﺮدن ﺳﻠﻮلﻫﺎي ﺑﻨﯿﺎدي ﻣﺸﺘﻖ از ﻣﻐﺰ اﺳﺘﺨﻮان روش اﻣﯿﺪوارﮐﻨﻨﺪهاي ﺑﺮاي اﻓﺰاﯾﺶ ﻗﺎﺑﻠﯿﺖ درﻣﺎﻧﯽ ﺳﻠﻮلﻫﺎي ﭘﯿﻮﻧﺪ ﺷﺪه ﻣﯽﺑﺎﺷﺪ. در اﯾﻦ ﻣﻄﺎﻟﻌﻪ اﺛﺮ درﻣﺎﻧﯽ ﺳﻠﻮلﻫﺎي ﺑﻨﯿﺎدي ﭘﯿﺶ ﺷﺮﻃﯽ ﺷﺪه ﺑﺎ دي ﻣﺘﯿﻞ اﮔﺰاﻟﯿﻞ ﮔﻠﯿﺴﯿﻦ )DMOG( ﺑﻪ ﻫﻤﺮاه ورزش ﺗﺮدﻣﯿﻞ را در ﻣﺪل آﻟﺰاﯾﻤﺮ ﺑﺮرﺳﯽ ﺷﺪ. ﻣﻮاد و روشﻫﺎ: ﺳﻠﻮلﻫﺎي ﺑﻨﯿﺎدي ﻣﺰاﻧﺸﯿﻤﯽ ﻣﺸﺘﻖ از ﻣﻐﺰ اﺳﺘﺨﻮان ﺑﻪ ﻣﺪت 24 ﺳﺎﻋﺖ ﺑﺎ DMOG ﺗﯿﻤﺎر و ﺳﭙﺲ از ﻃﺮﯾﻖ ورﯾﺪ دﻣﯽ ﺑﻪ ﻣﻮشﻫﺎي ﺻﺤﺮاﺋﯽ ﻣﺪل آﻟﺰاﯾﻤﺮي ﺗﺰرﯾﻖ ﺷﺪﻧﺪ. در ﮐﻨﺎر ﭘﯿﻮﻧﺪ ﺳﻠﻮل، ﺣﯿﻮاﻧﺎت ﺑﻪ ﻣﺪت ﯾﮏ ﻣﺎه روي ﺗﺮدﻣﯿﻞ ورزش ﮔﺮﻓﺘﻨﺪ. ﺳﭙﺲ ﺑﯿﺎن ژنﻫﺎي bax, bcl-2, Nrf2, and NQO1 روش Real-time PCR ﺑﺮرﺳﯽ ﮔﺮدﯾﺪ. ﯾﺎﻓﺘﻪ ﻫﺎ: ﭘﯿﻮﻧﺪ ﺳﻠﻮلﻫﺎي ﭘﯿﺶ ﺷﺮﻃﯽ ﺷﺪه ﺗﺎﺛﯿﺮ ﺑﯿﺶﺗﺮي در اﻓﺰاﯾﺶ ﺑﯿﺎن ژنﻫﺎي ﻓﻌﺎلﮐﻨﻨﺪه آﻧﺘﯽاﮐﺴﯿﺪان )Nrf2, NQO1( و ﮐﺎﻫﺶ آﭘﭙﺘﻮزﯾﺲ در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﺳﻠﻮل ﻏﯿﺮ ﺷﺮﻃﯽ داﺷﺖ. در ﻧﻬﺎﯾﺖ، اﻧﺠﺎم ورزش ﺗﺮدﻣﯿﻞ ﺑﻪ ﻣﺪت ﯾﮏ ﻣﺎه در ﮐﻨﺎر ﭘﯿﻮﻧﺪ ﺳﻠﻮلﻫﺎي ﭘﯿﺶ ﺷﺮﻃﯽ ﺷﺪه ﺑﺎ DMOG ﺑﯿﺶﺗﺮﯾﻦ ﺗﺎﺛﯿﺮ ﺣﻔﺎﻇﺘﯽ ﻋﺼﺒﯽ را ﻧﺸﺎن داد. نتيجه گيري با توجه به اثربخشي درمان هاي مبتني بر ذهن آگاهي، لزوم توجه بيش تر سياست گذاران و درمانگران اعتياد به اين شيوه درماني احساس مي شود.

Preconditioning of mesenchymal stem cells (MSCs) is a promising strategy to enhance the therapeutic properties of transplanted MSCs. In this study, we investigated the synergistic effects of treadmill exercise and dimethyloxalylglycine (DMOG)-preconditioned stem cells in an Alzheimer’s disease (AD) model. Materials and Methods DMOG- treated MSCs were intravenously transplanted in the AD model and the rats went on treadmill exercise for one month. Thusly, expression of bax, bcl-2, Nrf2, and NQO1 genes were assessed using real- time PCR. Results Transplantation of DMOG-treated cells had greater effects on increasing Nrf-2 and NQ1 expression and reducing apoptosis compared to non- treated cells. Ultimately, treadmill exercises for one month along with the transplantation of DMOG- treated cells showed the highest neuroprotective effect. Conclusion It seems that the transplantation of DMOG-treated cells beside exercise may have protective effects in the AD model via an increase in antioxidant capacity and a decrease in apoptosis