تأثير هيدروالكلي عصارة گياه آب تره بر ميزان قند خون موش سوري نر ديابتي

The effect of hydroalcoholic extract of Nasturtium officinale on blood glucose of diabetic mice

ﺎﺑﻘﻪ ﻭ ﻫﺪﻑ: ﺍﺯ ﺁﻧﺠﺎ ﻛﻪ ﺩﻳﺎﺑﺖ ﺷﺎﻳﻊ ﺗﺮﻳﻦ ﺑﻴﻤﺎﺭﻱ ﻏﺪﺩ ﺩﺭﻭﻥ ﺭﻳﺰ ﺍﺳﺖ ﻭ ﺭﻭﻧﺪ ﺭﻭ ﺑﻪ ﺭﺷﺪ ﺁﻥ ﻧﮕﺮﺍﻧﻲ ﻫﺎﻳﻲ ﺭﺍ ﺩﺭ ﻋﺮﺻﺔ ﺳﻼﻣﺖ ﻛﺸﻮﺭ ﺍﻳﺠﺎﺩ ﻛﺮﺩﻩ ﺍﺳـﺖ ﺍﺳـﺘﻔﺎ ﺩﻩ ﺍﺯ ﮔﻴﺎﻫﺎﻥ ﺩﺍﺭﻭﻳﻲ ﻣﻤﻜﻦ ﺍﺳﺖ ﺑﻪ ﺗﻨﻬﺎﻳﻲ ﻭ ﻳﺎ ﺩﺭ ﻛﻨﺎﺭ ﺩﺍﺭﻭﻫﺎﻱ ﺷﻴﻤﻴﺎﻳﻲ ﺩﺭ ﺩﺭﻣﺎﻥ ﺩﻳﺎﺑﺖ ﻣﺆﺛﺮ ﺑﺎﺷﺪ. ﻟﺬﺍ ﺑﻪ ﻣﻨﻈﻮﺭ ﺗﺄﺛﻴﺮ ﻋﺼﺎﺭﺓ ﮔﻴﺎﻩ ﺁﺏ ﺗـﺮﻩ ) Nasturtium officinale( ﺑﺮ ﻣﻴﺰﺍﻥ ﻗﻨﺪ ﺧﻮﻥ ﻣﻮﺵ ﺳﻮﺭﻱ ﺩﻳﺎﺑﺘﻲ ﺍﻳﻦ ﺗﺤﻘﻴﻖ ﺍﻧﺠﺎﻡ ﮔﺮﻓﺖ. ﻣﻮﺍﺩ ﻭﺭﻭﺵ ﻫﺎ: ﺩﺭ ﺍﻳﻦ ﻣﻄﺎﻟﻌﺔ ﺗﺠﺮﺑﻲ 40 ﺳﺮ ﻣﻮﺵ ﺳﻮﺭﻱ ﻧﺮ ﺑﺎ ﻭﺯﻥ 20-25 ﮔﺮﻡ ﭘﺲ ﺍﺯ ﺳﺎﺯﮔﺎﺭﻱ ﺑﺎ ﻣﺤﻴﻂ ﺣﻴﻮﺍﻥ ﺧﺎﻧﻪ ﺩﺭ ﺷﺮﺍﻳﻂ ﺍﺳﺘﺎﻧﺪﺍﺭﺩ ﺑﻪ ﺻـﻮﺭﺕ ﺗﺼـﺎﺩﻓﻲ ﺑﻪ 5 ﮔﺮﻭﻩ 8 ﺗﺎﻳﻲ ﺗﻘﺴﻴﻢ ﺷﺪﻧﺪ. 4 ﮔﺮﻭﻩ ﺑﻪ ﻭﺳﻴﻠﻪ ﺗﺰﺭﻳﻖ ﺩﺍﺧﻞ ﺻﻔﺎﺗﻲ ﺩﺍﺭﻭﻱ STZ )ﺍﺳﺘﺮﭘﺘﻮﺯﻳﺘﻮﺳﻴﻦ( ﺑﻪ ﻣﻴﺰﺍﻥ mg/kg 60 ﻭ ﻳﻚ ﮔﺮﻭﻩ ﺑﻪ ﻋﻨـﻮﺍﻥ ﮔـﺮﻭﻩ ﺷـﺎﻫﺪ ﻏﻴـﺮ ﺩﻳﺎﺑﺘﻲ ﺩﺭ ﻧﻈﺮ ﮔﺮﻓﺘﻪ ﺷﺪ. ﻗﺒﻞ ﻭ ﻫﻤﭽﻨﻴﻦ ﺩﺭ ﻓﻮﺍﺻﻞ 24، 72 ﺳﺎﻋﺘﻪ ﻭ ﻳﻚ ﻫﻔﺘﻪ ﺑﻌﺪ ﺍﺯ ﺗﺰﺭﻳﻖ stz ﻭ ﺗﺠﻮﻳﺰ ﻋﺼﺎﺭﺓ ﻫﻴﺪﺭﻭﺍﻟﻜﻠﻲ ﮔﻴﺎﻩ ﺁﺏ ﺗﺮﻩ، ﻗﻨﺪ ﺧﻮﻥ ﺑﺮﺭﺳﻲ ﺷـﺪ. ﺳﻪ ﮔﺮﻭﻩ ﺍﻭﻝ ﻋﺼﺎﺭﺓ ﮔﻴﺎﻩ ﺁﺏ ﺗﺮﻩ ﺭﺍ ﺑﺎ ﺳﻪ ﺩﻭﺯ ﻣﺘﻔﺎﻭﺕ )100 mg/kg، 300، 600 ﺩﺭﻳﺎﻓﺖ ﻛﺮﺩﻧﺪ. ﻗﻨﺪ ﺧﻮﻥ ﻫﺮ ﻳﻚ ﺍﺯ ﺍﻳﻦ ﺳﻪ ﮔﺮﻭﻩ ﺩﺭ ﺳﻪ ﻧﻮﺑﺖ ﺑﺎ ﻓﻮﺍﺻـﻞ ﻣﻌـﻴﻦ ﺍﻧﺪﺍﺯﻩ ﮔﻴﺮﻱ ﺷﺪ. ﻧﺘﺎﻳﺞ ﺑﻪ ﺩﺳﺖ ﺁﻣﺪﻩ ﺑﺎ ﺍﺳﺘﻔﺎﺩﻩ ﺍﺯ ﺁﺯﻣﻮﻥ t-test ﺩﺭ ﻧﺮﻡ ﺍﻓﺰﺍﺭ 16.spss v ﻣﻮﺭﺩ ﺗﺠﺰﻳﻪ ﻭ ﺗﺤﻠﻴﻞ ﺁﻣﺎﺭﻱ ﻗﺮﺍﺭ ﮔﺮﻓﺖ. ﻳﺎﻓﺘﻪ ﻫﺎ: ﻗﻨﺪ ﺧﻮﻥ ﮔﺮﻭﻩ ﻫﺎﻱ ﺗﻴﻤﺎﺭﺷﺪﻩ ﺑﺎ ﺩﻭﺯﻫﺎﻱ ﻣﺨﺘﻠﻒ ﺑﻮﻻﻍ ﺍﻭﺗﻲ )ﺁﺏ ﺗﺮﻩ( ﺩﺭ ﺯﻣﺎﻥ ﻫﺎﻱ 24 ﻭ 72 ﺳﺎﻋﺖ ﻭ ﻳﻚ ﻫﻔﺘﻪ، ﻛﺎﻫﺶ ﻣﻌﻨﺎﺩﺍﺭﻱ ﻧﺴﺒﺖ ﺑـﻪ ﮔـﺮﻭﻩ ﺷـﺎﻫﺪ ﺩﺭ ﺯﻣﺎﻥ ﻫﺎﻱ ﻣﺬﻛﻮﺭ ﺩﺍﺷﺖ )p<0.01(. ﺑﻪ ﻃﻮﺭﻱ ﻛﻪ ﻗﻨﺪ ﺧﻮﻥ ﮔﺮﻭﻩ ﻫﺎﻱ ﺩﻳﺎﺑﺘﻲ ﺷﺪﻩ ﺩﺭﻳﺎﻓﺖ ﻛﻨﻨﺪﺓ ﻋﺼﺎﺭﻩ، ﺩﺭ ﺯﻣﺎﻥ 72 ﺳﺎﻋﺖ ﻧﺴـﺒﺖ ﺑـﻪ 24 ﺳـﺎﻋﺖ ﻭ ﺩﺭ 1 ﻫﻔﺘـﻪ ﻧﺴﺒﺖ ﺑﻪ 72 ﺳﺎﻋﺖ ﻛﺎﻫﺶ ﺑﻴﺸﺘﺮﻱ ﺭﺍ ﻧﺸﺎﻥ ﺩﺍﺩ p<0.01(. ﺑﻴﺸﺘﺮﻳﻦ ﻛﺎﻫﺶ ﻗﻨﺪ ﺧﻮﻥ ﺩﺭ ﻏﻠﻈﺖ mg/kg 300 ﺑﻮﺩ. ﻧﺘﻴﺠﻪ ﮔﻴﺮﻱ: ﺑﻪ ﻧﻈﺮ ﻣﻲ ﺭﺳﺪ ﻛﻪ ﻋﺼﺎﺭﺓ ﺁﺏ ﺗﺮﻩ ﻧﻘﺶ ﻛﺎﻫﻨﺪﻩ ﺍﻱ ﺩﺭ ﻣﻴﺰﺍﻥ ﻗﻨﺪ ﺧﻮﻥ ﻣﻮﺵ ﻫﺎﻱ ﻧﺮ ﺩﻳﺎﺑﺘﻲ ﺩﺍﺷﺖ. ﺑﺮﺭﺳﻲ ﺁﻥ ﺭﻭﻱ ﺍﻧﺴﺎﻥ ﻫﺎ ﺗﻮﺻﻴﻪ ﻣﻲ ﺷﻮﺩ.

Background and Purpose: Since diabetes is the most common endocrine disorder, and its growing trend has raised concerns in the healthcare system, the use of medicinal herbs may be effective in treating diabetes alone or in combination with chemical drugs. Therefore, the aim of this study was to investigate the effect of Nasturtium officinal extract on blood glucose in diabetic rats. Methods and Materials: In this experimental study, 40 male mice with an average weight of 25-20 Grams after sustainability in standard animal house were divided randomly into 5 groups of 8 animals. Four drug groups by intraperitoneal injection of STZ (Streptozotocin) to the extent 60mg / kg and a non-diabetic control group were considered. Blood sugar was analyzed before and at 24, 72 hours and one week after injection of STZ and administration of hydroalcoholic Watercress extract. The first three groups received Watercress extract with three different doses (100, 300, 600 mg/kg). Data was analyzed using SPSS v.16 software. Paired t-test was used for statistical analysis. Results: Blood sugar of groups treated with different doses of NF (Watercress) at 24 and 72 hours and a week after was significantly lower than the control group at the same time (p<0.05) so that blood sugar groups extract-treated diabetic, took 72 hours to 24 hours compared to 72 hours in one week showed a greater reduction (p<0.05) greater drop in blood glucose concentration 300 mg/kg respectively.