ارزيابي خاصيت آنتي اكسيداني و سميت سلولي عصاره اتانولي گل شاهدانه (Cannabis sativa) بر روي سرطان پستان رده سلولي MDA-MB-231

Evaluation of antioxidant activity and cytotoxicity of an ethanol extract of Cannabis sativa on human breast cancer MDA-MB-231 cell line

مقدمه: سرطان سينه دومين سرطان شايع در خانم ها است. فرآورده­ هاي طبيعي استخراج شده از گياهان دارويي مي‌­تواند نقش مهمي در درمان سرطان داشته باشند. اين مطالعه به منظور بررسي خاصيت آنتي اكسيداني و سميت سلولي عصاره اتانولي گياه شاهدانه بر رده سلولي سرطاني سينه انجام شده است. روش ها: پس از كشت و تكثير رده سلولي MDA-MB-231، سلول­ها در مجاورت غلظت­ هاي مختلف عصاره اتانولي شاهدانه (μg/ml 200 و100، 50، 25) قرار گرفتند و به مدت 24، 48 و 72 ساعت انكوبه شدند. سپس جهت تعيين سميت سلولي عصاره از روش آزمون رنگ سنجي MTT استفاده شد. همچنين ميزان كل فنل، فلاونوئيد، آنتوسيانين،كاروتنوئيد و فعاليت آنتي اكسيداني به روش به دام اندازي راديكال دي فنيل پيكريل هيدرازيل (DPPH) نيز اندازه­‌گيري شد. يافته‌ها: نتايج نشان داد كه عصاره اتانولي در غلظت­ هاي مختلف، رشد سلول­ها را به طور معني­داري نسبت به گروه كنترل كاهش داد (0/05

Introduction: Breast cancer is the second most common cancer in the women. Natural products extracted from medicinal plants can play an important role in cancer treatment. This study investigated antioxidant and cytotoxic activity of an ethanol extract of Cannabis sativa in a human breast cancer cell line. Methods: MDA-MB-231cell line was cultivated and proliferated. Then, the cells exposed to different concentrations of C. sativa (25, 50, 100 and 200 μg/ml) and were incubated for 24, 48, and 72 hours. After the incubation period, the colorimetric MTT method was used to determine cytotoxicity. The total phenol, flavonoid, anthocyanin, and carotenoid contents of the extract as well as the antioxidant activity using DPPH method were determined. Results: The ethanol extract significantly decreased growth of cells compared to control (p ˂ 0.05). The maximum growth inhibition obtained 96.99% at the concentration 200 μg/ml. The extract of C. sativa is a rich source of antioxidant compounds. The phenol and carotenoid compounds of the extract were equal to 29.94± 0.12 mg galic acid/g of dried weight, and 31.93 ±0.18 mg galic acid/g of wet weight of the plant, respectively. Furthermore, the extract significantly inhibited DPPH radicals and the highest inhibitory effect (51%) was obtained by 2.3 mg/ml of the extract. Conclusion: These results suggest that the ethanol extract of Cannabis sativa has significant antioxidant and cytotoxic activity against MDA-MB-231 cell line. Further studies are needed to clarify potential anticancer effects of its ingredients.