اﺛﺮ ﺳﻤﯿﺖ ﺑﺮﺧﯽ ﻣﻮاد ﺿﺪ اﻧﺠﻤﺎد ﺑﺮ ﻧﺮخ ﺗﻔﺮﯾﺦ ﺟﻨﯿﻦ ﻣﺎﻫﯽ ﻗﺮهﺑﺮون Acipenser persicus

The effect of some cryoprotectants on the hatching rate of sturgeon embryos (Acipenser persicus)

اﻧﺠﻤﺎد ﺟﻨﯿﻦ ﻋﺒﺎرﺗﻨﺪ از ﺟﺎﯾﮕﺰﯾﻦ ﮐﺮدن ﻣﻮاد ﺿﺪ اﻧﺠﻤﺎد ﺑﺎ آب درون ﺟﻨﯿﻦ اﺳﺖ. اﻧﺠﻤﺎد ﻣﻮﻓﻘﯿﺖآﻣﯿﺰ ﺟﻨﯿﻦ ﻣﺎﻫﯿﺎن ﻧﯿﺎزﻣﻨﺪ ورود ﻣﻘﺎدﯾﺮ و ﻏﻠﻈﺖﻫﺎي ﻣﻨﺎﺳﺒﯽ از ﻣﻮاد ﺿﺪ اﻧﺠﻤﺎد ﺑﻪ ﻗﺴﻤﺖﻫﺎي ﻣﺨﺘﻠﻒ ﺟﻨـﯿﻦ اﺳـﺖ . در ﻣﻄﺎﻟﻌـﻪ ﺣﺎﺿـﺮ ﺑﻪﻣﻨﻈﻮر ﺑﺮرﺳﯽ اﺛﺮ ﺳﻤﯿﺖ ﻣﻮاد ﺿﺪ اﻧﺠﻤﺎد، ﺟﻨﯿﻦ ﻣﺎﻫﯽ ﻗﺮهﺑﺮون در ﻣﺮﺣﻠﻪ ﻣـﻮروﻻ ) 6 ﺳـﺎﻋﺖ ﭘـﺲ از ﻟﻘـﺎح ( ﻣـﻮرد ﺑﺮرﺳﯽ ﻗﺮار ﮔﺮﻓﺖ. از ﻣﻮاد ﺿﺪ اﻧﺠﻤﺎد ﻧﻔﻮذﭘﺬﯾﺮ؛ ﻣﺘﺎﻧﻮل، دي ﻣﺘﯿﻞ ﺳﻮﻟﻔﻮﮐﺴﺎﯾﺪ و ﺗﺮﮐﯿﺐ اﺗﯿﻠﻦ ﮔﻠﯿﮑﻮل ﺑﻪ ﻫﻤـﺮاه دي ﻣﺘﯿﻞ ﺳﻮﻟﻔﻮﮐﺴﺎﯾﺪ در ﻏﻠﻈﺖﻫﺎي )0/5، 0/75 و 1 ﻣﻮﻻر( و ﺑﻪ ﻫﻤﺮاه ﻣﻮاد ﺿـﺪ اﻧﺠﻤـﺎد ﻧﻔﻮذﻧﺎﭘـﺬ ﯾﺮ ﺳـﻮﮐﺮوز اﺳـﺘﻔﺎده ﮔﺮدﯾﺪ. ﺗﺨﻢﻫﺎ در ﻃﯽ 3 ﻣﺮﺣﻠﻪ و ﻫﺮ ﻣﺮﺣﻠﻪ ﻧﯿﺰ ﺑﻪ ﻣﺪت 5 دﻗﯿﻘﻪ در ﻣﻌﺮض ﻣﺤﻠﻮلﻫﺎ ﺑﻪ ﺗﺮﺗﯿـﺐ از ﻣﻮﻻرﯾﺘـﻪ ﮐـﻢ ﺑـﻪ ﻣﻮﻻرﯾﺘﻪ ﺑﺎﻻ ﻗﺮار ﮔﺮﻓﺘﻪ و ﺗﺎ زﻣﺎن ﺗﻔﺮﯾﺦ در اﻧﮑﻮﺑﺎﺗﻮرﻫﺎ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. از ﻟﺤﺎظ ﻧﺮخ ﺗﻔﺮﯾﺦ اﺧﺘﻼف ﻣﻌﻨﯽدار آﻣﺎري ﺑـﯿﻦ ﻣﻮاد ﺿﺪ اﻧﺠﻤﺎد ﻣﺨﺘﻠﻒ اﻧﺘﺨﺎب ﺷﺪه، در ﻫﺮ ﺳﻪ ﺗﯿﻤﺎر در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﮔﺮوه ﺷـﺎﻫﺪ وﺟـﻮد ﻧﺪاﺷـﺖ ) 0/05>P و ﺗﺤـﺖ آزﻣﻮن داﻧﮑﻦ(. اﮔﺮ ﭼﻪ از ﻣﯿﺎن ﻣﻮاد ﺿﺪ اﻧﺠﻤﺎد ﻧﻔﻮذﭘﺬﯾﺮ، ﺗﺮﮐﯿـﺐ اﺗـﯿﻠﻦ ﮔﻠﯿﮑـﻮل ﺑـﻪ ﻫﻤـﺮاه دي ﻣﺘﯿـﻞ ﺳﻮﻟﻔﻮﮐﺴـﺎﯾﺪ ﺑﯿﺸﺘﺮﯾﻦ ﺳﻤﯿﺖ را ﺑﺮاي ﺟﻨﯿﻦ ﻣﺎﻫﯽ ﻗﺮهﺑﺮون داﺷﺖ. ﻫﻤﭽﻨﯿﻦ ﺳﻄﻮح ﻣﺨﺘﻠﻒ ﻣﺘﺎﻧﻮل را ﻧﺴﺒﺖ ﺑﻪ ﺗﺮﮐﯿﺐ اﺗﯿﻠﻦ ﮔﻠﯿﮑﻮل ﺑﻪ ﻫﻤﺮاه دي ﻣﺘﯿﻞ ﺳﻮﻟﻔﻮﮐﺴﺎﯾﺪ ﺑﻬﺘﺮ ﺗﺤﻤﻞ ﮐﺮده و ﻧﺴﺒﺖ ﺑﻪ آن ﺣﺴﺎﺳﯿﺖ ﮐﻤﺘﺮي داﺷﺖ اﻣﺎ ﺟﻨﯿﻦ ﻣﺎﻫﯽ ﻣﻮرد ﻣﻄﺎﻟﻌـﻪ ﺳﻄﻮح ﻣﺨﺘﻠﻒ از ﻣﻮاد دي ﻣﺘﯿﻞ ﺳﻮﻟﻔﻮﮐﺴﺎﯾﺪ را ﻧﺴﺒﺖ ﺑﻪ دو ﻣﺎده دﯾﮕﺮ ﺑﻬﺘﺮ ﺗﺤﻤﻞ ﮐﺮده و ﺣﺴﺎﺳﯿﺖ ﮐﻤﺘﺮي ﻧﺴﺒﺖ ﺑ آنها نشان دادند.

Cryopreservation of fish embryo is replacement of cryoprotectants with water in side embryo. Cryopreservation of fish embryos requires an optimal concentration of cryoprotectants inside all embryo compartments. At this study toxicity of cryoprotectants, fish embryos were examined in stage (6 hours after fertilization).In the present study, to evaluate the cryoprotectant toxicity was determined in sturgeon embryos at morula stage (6 hours after fertilization). From permeable cryoprotectants, methanol, dimethyl sulfoxide and combination of dimethyl sulfoxide with ethylene glycol in concentrations (0.5, 0.75, and 1 M) and non-permeable, sucrose was used. Embryos treated in three stage and for each stage about 5 minutes from low to above molarity, and the embryos were incubated until hatching. There were no significant differences between cryoprotectants differences concentrations, in terms of hatching rate in each of three treatment compared with the controls (P>0.05 and under Duncan). However among the permeable cryoprotectants, combination of dimethyl sulfoxide with ethylene glycol had the highest toxicity for sturgeon embryos. But embryos better tolerated differences concentrations of dimethyl sulfoxide than two other cryoprotectants and less sensitive than.