ارزيابي اثر حفاظتي روغن سياه دانه بر بافت كبد موش هاي نرNMRI متعاقب سميت نانو ذرات نقره

ﺎوﺟﻮد اﺳﺘﻔﺎده ﮔﺴﺘﺮده از ﻧﺎﻧﻮ ذرات ﻧﻘﺮه ﻧﮕﺮاﻧﻲ ﻫﺎﻳﻲ در ﻣﻮرد اﺛﺮات ﺑﻴﻮﻟﻮژﻳﻚ آن ﻫﺎ ﺑﺮ روي ﺳﻼﻣﺖ اﻧﺴﺎن وﺟﻮد دارد. ﻫﺪف از اﻳﻦ ﻣﻄﺎﻟﻌﻪ ﺑﺮرﺳﻲ اﺛﺮ روﻏﻦ ﺳﻴﺎه داﻧﻪ ﺑﺮ ﺑﺎﻓﺖ ﻛﺒﺪ ﻣﻮش در ﭘﻲ ﺳﻤﻴﺖ ﻧﺎﻧﻮ ذرات ﻧﻘﺮه ﺑﻮد. 24 ﺳﺮ ﻣﻮش ﻧﺮ ﺑﺎﻟﻎ ﻧﮋاد NMRI ﺑﻪ ﭼﻬﺎر ﮔﺮوه ﻛﻨﺘﺮل، ﻧﺎﻧﻮ ذرات ﻧﻘﺮه )mg/kg/day 500، ﮔﺎواژ(، روﻏﻦ ﺳﻴﺎه داﻧﻪ ml/kg/day 5، ﮔﺎواژ( آﻣﻴﻨﻮ ﺗﺮاﻧﺴﻔﺮاز ALT، آﺳﭙﺎرﺗﺎت آﻣﻴﻨﻮﺗﺮاﻧﺴﻔﺮاز (AST و ﻣﺎﻟﻮن ديآﻟﺪﺋﻴﺪ MDA ﻧﻴﺰ اﻧﺪازه ﮔﻴﺮي ﺷﺪ. داده ﻫﺎ ﺑﺎ اﺳﺘﻔﺎده از آزﻣﻮن آﻧﺎﻟﻴﺰوارﻳﺎﻧﺲ ﻳﻚ ﻃﺮﻓﻪ ﺗﺠﺰﻳﻪ وﺗﺤﻠﻴﻞ آﻣﺎري ﺷﺪ و ﺗﻔﺎوت ﻣﻴﺎﻧﮕﻴﻦ ﻫﺎ در ﺣﺪ P<0/05 ﻣﻌﻨﻲ دار در ﻧﻈﺮ ﮔﺮﻓﺘﻪ ﺷﺪ. ﻛﺎﻫﺶ ﻣﻌﻨﻲ داري در ﻣﻴﺎﻧﮕﻴﻦ وزن ﻧﺴﺒﻲ ﻛﺒﺪ، ﺣﺠﻢ ﻛﻞ ورﻳﺪ ﻣﺮﻛﺰي، ﺣﺠﻢ ﻛﻞ ﺷﺮﻳﺎن، ﺑﺎﻓﺖ ﻫﻤﺒﻨﺪ و ورﻳﺪ دﺳﺘﮕﺎه ﭘﻮرﺗﺎل و اﻓﺰاﻳﺶ ﻣﻌﻨﻲ داري در ﺣﺠﻢ ﻛﻞ ﺳﻴﻨﻮزوﺋﻴﺪﻫﺎ، ﻣﺠﺮاي ﺻﻔﺮاوي، ﺣﺠﻢ ﺳﻠﻮل ﻫﭙﺎﺗﻮﺳﻴﺖ و ﺳﻄﻮح MDA و آﻧﺰﻳﻢ ﻫﺎي ALT و AST در ﮔﺮوه ﻧﺎﻧﻮ ذرات ﻧﻘﺮه ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﻛﻨﺘﺮل ﻣﺸﺎﻫﺪه ﺷﺪ P<0/05 . اﻛﺜﺮ آﺳﻴﺐ ﻫﺎي ﻛﺒﺪي اﻟﻘﺎﺷﺪه ﺗﻮﺳﻂ ﻧﺎﻧﻮ ذرات ﻧﻘﺮه، در ﮔﺮوه ﻧﺎﻧﻮ ذرات ﻧﻘﺮه + ﺳﻴﺎه داﻧﻪ در ﺣﺪ ﮔﺮوه ﻛﻨﺘﺮل ﺑﻬﺒﻮد ﻳﺎﻓﺖ. ﻧﺘﺎﻳﺞ ﺣﺎﺻﻞ از اﻳﻦ ﻣﻄﺎﻟﻌﻪ ﻧﺸﺎن داد ﻛﻪ روﻏﻦ ﺳﻴﺎه داﻧﻪ ﻣﻲ ﺗﻮاﻧﺪ ﻛﺒﺪ را از آﺳﻴﺐ ﻧﺎﻧﻮ ذرات ﻧﻘﺮه ﻣﺤﺎﻓﻈﺖ ﻛﻨﺪ.

Despite the widespread use of silver nanoparticles (SNP), there are concerns about their biological effects on the human health. The aim of this study was to investigate the effect of Nigella sativa oil (NSO) on mice liver following silver nanoparticles toxicity. 24 adult male NMRI mice were divided into 4 groups: control, SNP (500mg/kg/day, gavage), NSO (5ml/kg/day, gavage) and finally SNP plus NSO and treated for 35 days. Histological assessment of liver were performed using stereological methods. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and malondialdehyde (MDA) were also measured. A significant decrease in the mean relative weight of liver, the total volume of the central veins, the total volume of the connective tissue, arteries and veins in the portal tracts and a significant increase in the total volume of sinusoids, bile ductules, the hepatocytes cell volume and serum levels of MDA and ALT and AST enzymes were observed in the silver nanoparticles group compared with the control group. The major of silver nanoparticles-induced Liver damages improved in the silver nanoparticles + Nigella sativa oil group to the control ones. The results of this study suggest that Nigella sativa oil can protect the liver from silver nanoparticles damage