عنوان مقاله :
بررﺳﯽ وﺿﻌﯿﺖ آراﯾﻪﺷﻨﺎﺳﯽ ﺟﻤﻌﯿﺖ ﮔﺎوﻣﺎﻫﯿﺎن )Teleost: Gobiidae( در ﺷﺶ رودﺧﺎﻧﻪ ﺣﻮﺿﻪ ﺟﻨﻮﺑﯽ درﯾﺎي ﺧﺰر ﺑﺮاﺳﺎس ژن ﺳﯿﺘﻮﮐﺮوم اﮐﺴﯿﺪاز COI
عنوان به زبان ديگر :
Taxonomic status of six populations of the Gobiids (Teleost, Gobiidae) in the southern Caspian Sea basin using COI gene
پديد آورندگان :
ﻧﯿﮏﻣﻬﺮ، ﻧﺴﺮﯾﻦ داﻧﺸﮕﺎه ﺗﻬﺮان - ﭘﺮدﯾﺲ ﮐﺸﺎورزي و ﻣﻨﺎﺑﻊ ﻃﺒﯿﻌﯽ ﮐﺮج - داﻧﺸﮑﺪهء ﻣﻨﺎﺑﻊ ﻃﺒﯿﻌﯽ , اﯾﮕﺪري، ﺳﻬﯿﻞ داﻧﺸﮕﺎه ﺗﻬﺮان - ﭘﺮدﯾﺲ ﮐﺸﺎورزي و ﻣﻨﺎﺑﻊ ﻃﺒﯿﻌﯽ ﮐﺮج - داﻧﺸﮑﺪهء ﻣﻨﺎﺑﻊ ﻃﺒﯿﻌﯽ - گروه شيلات , ﭘﻮرﺑﺎﻗﺮ، ﻫﺎدي داﻧﺸﮕﺎه ﺗﻬﺮان - ﭘﺮدﯾﺲ ﮐﺸﺎورزي و ﻣﻨﺎﺑﻊ ﻃﺒﯿﻌﯽ ﮐﺮج - داﻧﺸﮑﺪهء ﻣﻨﺎﺑﻊ ﻃﺒﯿﻌﯽ - گروه شيلات , ﯾﻮﺳﻔﯽ، آرﻣﯿﻦ داﻧﺸﮕﺎه ﺗﻬﺮان - ﭘﺮدﯾﺲ ﮐﺸﺎورزي و ﻣﻨﺎﺑﻊ ﻃﺒﯿﻌﯽ ﮐﺮج - داﻧﺸﮑﺪهء ﻣﻨﺎﺑﻊ ﻃﺒﯿﻌﯽ
كليدواژه :
ﮔﺎوﻣﺎﻫﯿﺎن , درﯾﺎي ﺧﺰر , ژن ﺳﯿﺘﻮﮐﺮوم اﮐﺴﯿﺪاز , آراﯾﻪﺷﻨﺎﺳﯽ
چكيده فارسي :
ﺑﻪدﻟﯿﻞ ﺷﺒﺎﻫﺖ رﯾﺨﺘﯽ ﺑﺎﻻ ﺑﯿﻦ ﺟﻤﻌﯿﺖ و ﮔﻮﻧﻪﻫﺎي ﺟﻨﺲ Ponticola در ﺳﻮاﺣﻞ ﺟﻨﻮﺑﯽ درﯾﺎي ﺧﺰر، ﺷﻨﺎﺳﺎﯾﯽ آنﻫﺎ از ﻟﺤﺎظ رﯾﺨﺖﺷﻨﺎﺳﯽ ﺑﺮاﺳﺎس ﮐﻠﯿﺪﻫﺎي ﺷﻨﺎﺳﺎﯾﯽ دﺷﻮار اﺳﺖ. اﻣﺮوزه ﻧﺸﺎﻧﮕﺮﻫﺎي ﻣﻮﻟﮑﻮﻟﯽ ﺑﻪﻋﻨﻮان روشﻫﺎي ﻣﮑﻤﻞ در ﺷﻨﺎﺧﺖ ﺑﻬﺘﺮ وﺿﻌﯿﺖ آراﯾﻪﺷﻨﺎﺧﺘﯽ ﻣﺎﻫﯿﺎن ﻣﻮرد اﺳﺘﻔﺎده ﻗﺮار ﻣﯽﮔﯿﺮد. در ﺗﺤﻘﯿﻖ ﺣﺎﺿﺮ ﺑﺮرﺳﯽ وﺿﻌﯿﺖ آراﯾﻪﺷﻨﺎﺳﯽ ﮔﺎوﻣﺎﻫﯿﺎن در ﺷﺶ رودﺧﺎﻧﻪ )ﺳﯿﺎﻫﺮود، زرﯾﻦﮔﻞ، ﺑﺎﺑﻠﺮود، ﺗﺠﻦ، ﺳﻔﯿﺪرود )دو ﺟﻤﻌﯿﺖ ﺑﯿﺠﺎر و ﮐﯿﺴﻮم(، ﮐﺴﻠﯿﺎن( در ﺣﻮﺿﻪ ﺟﻨﻮﺑﯽ درﯾﺎي ﺧﺰر ﺑﺮاﺳﺎس روش ﺷﻨﺎﺳﻪﮔﺬاري )ﺑﺎرﮐﺪﯾﻨﮓ( DNA ﺑﺎ اﺳﺘﻔﺎده از ژن COI ﻣﯿﺘﻮﮐﻨﺪرﯾﺎﯾﯽ ﺑﺎ ﺗﺮﺳﯿﻢ درﺧﺖ ﺷﺠﺮهﺷﻨﺎﺳﯽ ﺑﺎ روشﻫﺎي Bayesian و Maximum Likelihood، ﺑﺮرﺳﯽ ﺷﺒﮑﻪ ﻫﺎﭘﻠﻮﺗﺎﯾﭙﯽ و ارﺗﺒﺎط ﻓﺎﺻﻠﻪ ﺟﻐﺮاﻓﯿﺎﯾﯽ و ﺗﻤﺎﯾﺰ ژﻧﺘﯿﮑﯽ اﻧﺠﺎم ﺷﺪ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﻣﯿﺎﻧﮕﯿﻦ ﻓﺎﺻﻠﻪ ژﻧﺘﯿﮑﯽ K2P ﺑﯿﻦ ﺟﻤﻌﯿﺖﻫﺎي ﻣﻮرد ﻣﻄﺎﻟﻌﻪ 0/69-1/68 اﺳﺖ ﮐﻪ ﺑﺮاﺳﺎس ﻣﻌﯿﺎرﻫﺎي ژﻧﺘﯿﮑﯽ ﺑﺮاي ﺗﻔﮑﯿﮏ ﮔﻮﻧﻪاي اﻧﺪك اﺳﺖ. ﻫﻤﭽﻨﯿﻦ دارﻧﮕﺎره ﺗﺮﺳﯿﻢ ﺷﺪه و ﺷﺒﮑﻪ ﻫﺎﭘﻠﻮﺗﺎﯾﭙﯽ ﺗﻤﺎﻣﯽ ﺟﻤﻌﯿﺖﻫﺎي ﻣﻮرد ﺑﺮرﺳﯽ را ﻫﺎﭘﻠﻮﺗﺎﯾﭗﻫﺎي ﮔﻮﻧﻪ P. gorlap ﻧﺸﺎن داد.
چكيده لاتين :
Morphological similarities of the populations and species of the genus Ponticola in the southern Caspian Sea make their identification difficult based on the provided keys. Molecular markers are used as a supplementary tools to better understanding of the fishes’ taxonomic statues. This study was conducted to understand taxonomic statues of gobiids from 6 rivers (Siah, Zaringol, Babol, Tajan, Sefid vis. Bijar and Kisum populations, and Kelsian) in Southern Caspian Sea basin based on DNA barcoding using mitochondrial COI gene by reconstructing phylogenetic tree based on ML and BI methods and drawing haplotype network and assessment of the geographic and genetic distances. The results show a mean genetic distance (K2P) of 0.69-1.68 between the studied populations that is too low for considering them as distinct species based on the genetic criteria. Also, BI and ML cladograms and haplotype network showed all populations are haplotypes of P. gorlap.
عنوان نشريه :
پژوهش هاي ماهي شناسي كاربردي
