بررسي اثر تيمارهاي تنظيم كننده رشد در بيوسنتز اندول الكالوييدهاي پروانش Catharanthus roseus (L) G. Don. در شرايط كشت بافت، كشت سوسپانسيوني و كشت مزرعه

Evaluation of the hormonal treatments effect on biosynthesis of endol alkaloids in tissue culture, suspension culture and field culture

ﮔﯿﺎه ﭘﺮواﻧﺶ ﻣﻨﺒﻊ ﻏﻨﯽ از آﻟﮑﺎﻟﻮﺋﯿﺪﻫﺎ اﺳﺖ ﮐﻪ در ﻫﻤﻪ ﺑﺨﺶﻫﺎي ﮔﯿﺎه ﭘﺮاﮐﻨﺪه اﻧﺪ. وﺟﻮد آﻟﮑﺎﻟﻮِ ﺋﯿﺪﻫﺎي ارزﺷﻤﻨﺪ در ﺗﻤﺎم ﺑﺨﺶ ﻫﺎي ﮔﯿﺎه ﺳﺒﺐ ﺷﺪه اﺳﺖ ﮐﻪ، ﺑﻪ ﻋﻨﻮان ﯾﮏ ﮔﯿﺎه داروﯾﯽ ﺑﺴﯿﺎر ﻣﻬﻢ ﻣﻌﺮﻓﯽ و ﺧﻮاص آن ﺑﺮرﺳﯽ ﺷﻮد.اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﯽ اﺛﺮ ﺗﯿﻤﺎرﻫﺎي ﺗﻨﻈﯿﻢ ﮐﻨﻨﺪه رﺷﺪي ﺑﺮ ﺑﯿﻮﺳﻨﺘﺰ اﻧﺪول اﻟﮑﺎﻟﻮﯾﯿﺪﻫﺎي ﮔﯿﺎه داروﯾﯽ ﭘﺮواﻧﺶ در ﺷﺮاﯾﻂ ﮐﺸﺖ ﺑﺎﻓﺖ، ﮐﺸﺖ ﺳﻮﺳﭙﺎﻧﺴﯿﻮﻧﯽ و ﮐﺸﺖ ﻣﺰرﻋﻪ اﺟﺮا ﺷﺪ.آزﻣﺎﯾﺶ در ﺳﻪ ﮐﺸﺖ ﺟﺪاﮔﺎﻧﻪ،ﺷﺎﻣﻞ ﮐﺸﺖ ﺑﺎﻓﺖ، ﮐﺸﺖ ﺳﻮﺳﭙﺎﻧﺴﯿﻮﻧﯽ، ﮐﺸﺖ ﻣﺰرﻋﻪ و ﺗﺎﺛﯿﺮ اﺳﺘﻔﺎده ﻫﻢ زﻣﺎن ﺗﻨﻈﯿﻢ ﮐﻨﻨﺪه ﻫﺎي رﺷﺪIAA و NAA ﺑﺮ ﻣﯿﺰان آﻟﮑﺎﻟﻮﺋﯿﺪﻫﺎي ﺗﻮﻟﯿﺪ ﺷﺪه ﺗﻮﺳﻂ ﮔﯿﺎه ﭘﺮواﻧﺶ اﻧﺠﺎم ﺷﺪ. ﻫﻮرﻣﻮن ﻫﺎي IAA در ﺳﻪ ﻏﻠﻈﺖ)0/1، 5/ 0، 1/0 ﻣﯿﻠﯽ ﮔﺮم در ﻟﯿﺘﺮ(و NAA )0/1، 0/5 1/0 ﻣﯿﻠﯽ ﮔﺮم در ﻟﯿﺘﺮ( در ﺳﻪ ﺗﮑﺮارﺑﺼﻮرت ﻓﺎﮐﺘﻮرﯾﻞ در ﻗﺎﻟﺐ ﻃﺮح ﮐﺎﻣﻼ ﺗﺼﺎدﻓﯽ ﻣﺰرﻋﻪ ﻣﻮﺳﺴﻪ ﺗﺤﻘﯿﻘﺎت اﺻﻼح و ﺗﻬﯿﻪ ﻧﻬﺎل و ﺑﺬر ﮐﺮج درﺳﺎل1398اﺟﺮا ﺷﺪ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ NAA، ﺑﺎﻋﺚ اﻓﺰاﯾﺶ ﻣﯿﺰان آﻟﮑﺎﻟﻮﺋﯿﺪ ﻫﺎي آﺟﻤﺎﻟﯿﺴﯿﻦ، ﺳﺮﭘﺎﻧﺘﯿﻦ و اﻓﺰاﯾﺶ ﻣﯿﺰان آﻟﮑﺎﻟﻮﺋﯿﺪ ﮐﻞ در ﺣﺪود 20 درﺻﺪ ﻣﯽ ﺑﺎﺷﺪ. در ﺑﯿﻦ ﺗﻨﻈﯿﻢ ﮐﻨﻨﺪه ﻫﺎي رﺷﺪ ﻣﻮرد ﻣﻄﺎﻟﻌﻪ، IAA اﺛﺮ ﮐﺎﻫﺸﯽ را در ﺗﻐﯿﯿﺮات ﻣﯿﺰان آﻟﮑﺎﻟﻮﺋﯿﺪﻫﺎ از ﺧﻮد ﻧﺸﺎن داد و اﯾﻦ ﮐﺎﻫﺶ در ﺑﺮﺧﯽ ﻣﻮارد در ﺣﺪود 10 درﺻﺪ ﺑﻮد. اﺳﺘﻔﺎده از روش ﮐﺸﺖ ﺳﻮﺳﭙﺎﻧﺴﯿﻮﻧﯽ در ﺑﺴﯿﺎري از ﻣﻮارد ﻧﺴﺒﺖ ﺑﻪ دو روش دﯾﮕﺮ ﺑﺎﻋﺚ اﻓﺰاﯾﺶ آﻟﮑﺎﻟﻮﺋﯿﺪﻫﺎ ﺷﺪ، و در ﮐﺸﺖ ﻣﺰرﻋﻪ ﻣﻘﺪار آﻟﮑﺎﻟﻮﺋﯿﺪ ﮐﻞ ﻧﺴﺒﺖ ﺑﻪ ﺳﺎﯾﺮ روش ﻫﺎ اﻓﺰاﯾﺶ ﻧﺸﺎن داد. ﺑﺮاﺳﺎس ﻧﺘﺎﯾﺞ ﺣﺎﺻﻞ از اﯾﻦ آزﻣﺎﯾﺶ اﺳﺘﻔﺎده از روش ﮐﺸﺖ ﺳﻮﺳﭙﺎﻧﺴﯿﻮﻧﯽ در ﺑﺴﯿﺎري از ﻣﻮارد ﻧﺴﺒﺖ ﺑﻪ دو ﮔﺮوه دﯾﮕﺮ ﻣﺰﯾﺖ ﻧﺴﺒﺘﺎ ﻣﻨﺎﺳﺐ ﺗﺮي را از ﺧﻮد ﻧﺸﺎن ﻣﯽدﻫﺪ.

The plant is a rich source of alkaloids that are distributed in all parts of the plant. The presence of valuable alkaloids in all parts of the plant has led to its introduction as a very important medicinal plant and its properties to be investigated. This study was conducted to investigate the effect of growth regulatory treatments on the biosynthesis of indole alkaloids of Parvanesh medicinal plant in culture. Tissue, suspension culture and field culture were performed. The experiment was performed in three separate cultures, including tissue culture, suspension culture, field culture and the effect of simultaneous use of IAA and NAA growth regulators on the amount of alkaloids produced by the butterfly plant. . IAA hormones in three concentrations (0.1, 0.5 mg / L) and NAA (0.1, 0.5 0.5 mg / L) in three replications in a completely randomized field design Karaj Seed and Plant Breeding Research Institute was established in 1398. The experimental results showed that NAA increased the alkaloids of agemalicin, serpentine and increased the total alkaloids by about 20%. Among the growth regulators studied, IAA showed a decreasing effect on changes in alkaloid levels, and in some cases this decrease was about 10%. Also, the use of suspension culture method increased alkaloids and in field cultivation, the amount of total alkaloids increased compared to other methods. Based on the results of this experiment, it seems that the use of method In many cases, suspension cultivation has a relatively better advantage than the other two groups.