تأثير تمرين تناوبي شديد بر آدروپين و فاكتور رشد اندوتليال عروقي در بافت ريه در رت هاي پير

The Effect of High Intensity Interval Training on Vascular Endothelial Growth Factor and Adropin in Lung Tissue in Old Rats

ﺑﺎ اﻓﺰاﯾﺶ ﺳﻦ، اﺧﺘﻼل ﻋﻤﻠﮑﺮد اﻧﺪوﺗﻠﯿﺎل ﮐﻪ ﮐﻠﯿﺪي ﺑﺮاي ﺑﻪ وﺟﻮد آﻣﺪن آﺗﺮوﺳﮑﻠﺮوز و آﻏﺎز ﻧﺎرﺳﺎﯾﯽ ﻗﻠﺒﯽ- ﺗﻨﻔﺴﯽ در ﺳﺎﻟﻤﻨﺪان اﺳﺖ، اﻓﺰاﯾﺶ ﻣﯽﯾﺎﺑﺪ. اﻓﺰاﯾﺶ ﺳﻦ ﻣﻮﺟﺐ اﻓﺰاﯾﺶ ﮐﺎر ﺗﻨﻔﺴﯽ، ﺗﻐﯿﯿﺮ ﺣﺠﻢﻫﺎي ﻋﻤﻠﮑﺮدي رﯾﻪ و ﺗﻨﮕﯽ ﻧﻔﺲ ﻣﯽ-ﮔﺮدد. ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ ﺑﻪ ﻣﻨﻈﻮر ﺗﻌﯿﯿﻦ ﺗﺎﺛﯿﺮ ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺑﺮ آدروﭘﯿﻦ و ﻓﺎﮐﺘﻮر رﺷﺪ اﻧﺪوﺗﻠﯿﺎل ﻋﺮوﻗﯽ )VEGF( ﺑﺎﻓﺖ رﯾﻪ رتﻫﺎي ﻧﺮ ﻣﺴﻦ اﻧﺠﺎم ﺷﺪ. اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﻪ روش ﺗﺠﺮﺑﯽ و ﺑﺮ روي 16 ﺳﺮ رت ﭘﯿﺮ )22 ﺗﺎ 24 ﻣﺎه( اﻧﺠﺎم ﺷﺪ. رتﻫﺎ ﺑﻪ ﺻﻮرت ﺗﺼﺎدﻓﯽ ﺑﻪ ﮔﺮوه ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ )HIIT( و ﮔﺮوه ﮐﻨﺘﺮل ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﺑﺮﻧﺎﻣﻪ ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ دوﯾﺪن ﺑﺮ روي ﺗﺮدﻣﯿﻞ ﺑﺎ ﺷﺪت 80-100 درﺻﺪ ﺣﺪاﮐﺜﺮ اﮐﺴﯿﮋن ﻣﺼﺮﻓﯽ ﺑﻮد. 48 ﺳﺎﻋﺖ ﭘﺲ از آﺧﺮﯾﻦ ﺟﻠﺴﻪ ﺗﻤﺮﯾﻦ، ﺣﯿﻮاﻧﺎت ﺑﺎ ﺗﺮﮐﯿﺒﯽ از ﮐﺘﺎﻣﯿﻦ و زاﯾﻼزﯾﻦ ﺑﯿﻬﻮش ﺷﺪﻧﺪ. ﺑﺎﻓﺖ رﯾﻪ ﺑﺮ روي ﯾﺦ ﺟﺪا و در 80- درﺟﻪ ﺳﺎﻧﺘﯿﮕﺮاد ﺗﺎ زﻣﺎن آزﻣﺎﯾﺶ ﻧﮕﻬﺪاري ﮔﺮدﯾﺪ. ﻗﺴﻤﺖ روﯾﯽ ﺑﺪﺳﺖ آﻣﺪه ﺣﺎﺻﻞ از ﻫﻤﻮژﻧﯿﺰاﺳﯿﻮن ﺑﺎﻓﺖ رﯾﻪ ﺑﺮاي ﺗﻌﯿﯿﻦ آدروﭘﯿﻦ و VEGF ﻣﻮرد اﺳﺘﻔﺎده ﻗﺮار ﮔﺮﻓﺖ. از آزﻣﻮن ﺗﯽ ﻣﺴﺘﻘﻞ ﺑﺮاي ﺗﻌﯿﯿﻦ ﺗﻔﺎوت ﺑﯿﻦ ﮔﺮوهﻫﺎ اﺳﺘﻔﺎده ﺷﺪ )P<0/05(. ﻓﺎﮐﺘﻮر رﺷﺪ اﻧﺪوﺗﻠﯿﺎل ﻋﺮوﻗﯽ و آدروﭘﯿﻦ در ﮔﺮوه ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل )P=0/001( اﻓﺰاﯾﺶ ﻣﻌﻨﺎداري ﯾﺎﻓﺖ. ﺑﻪ ﻃﻮر ﮐﻠﯽ، ﺗﻤﺮﯾﻦ ﺗﻨﺎوﺑﯽ ﺷﺪﯾﺪ ﺑﺎﻋﺚ اﻓﺰاﯾﺶ ﻣﯿﺰان ﻓﺎﮐﺘﻮر آﻧﮋﯾﻮژﻧﺰ VEGF و آدروﭘﯿﻦ در رت ﻫﺎي ﻧﺮ ﻣﺴﻦ ﻣﯽﮔﺮدد.

As the age increases (during aging process), endothelial dysfunction which is a key to atherosclerosis and the onset of cardio-respiratory failure in the elderly increases. Aging increases respiratory function, changes in lung function and shortness of breath. The present study was performed to determine the effect of high intensity interval training on vascular endothelial growth factor (VEGF) and adropin in lung tissue in old rats. This experimental study was performed on 16 old rats (22-24 months). The rats were randomly divided into high intensity interval training (HIIT) and control groups. A routine interval training program was conducted on a treadmill with an intensity of 100-80% of maximum oxygen consumption, 5 sessions per week for 6 weeks. 48 hours after the last exercise session, animals were anesthetized with a combination of ketamine and Xylacin. The lung tissue was isolated on ice and kept at -80 °C until the test. The supernatant was used to determine the levels of VEGF and adropin. Independent T-Test was used to determine the difference between groups (P <0.05). Results: VEGF and adropin were increased significantly in high intensity interval training group compared to control group. In general, high intensity interval training increases VEGF and adropin in old male rats