اثرات سطوح مختلف اسيدآمينه تورين در جيره بر رنگ پذيري ماهي رزي بارب (Pethia conchonius)

Effects of different levels of dietary taurine amino acid on Rosy Barb (Pethia conchonius) pigmentation

ﭼﮑﯿﺪه ﺗﻮرﯾﻦ ) 2آﻣﯿﻨﻮ اﺗﺎن ﺳﻮﻟﻔﻮﻧﯿﮏ اﺳﯿﺪ(، ﺟﺰء آﻣﯿﻨﻮاﺳﯿﺪﻫﺎي ﻧﯿﻤﻪﺿﺮوري و ﻣﺤﺼﻮل ﻧﻬﺎﯾﯽ ﺳﻮﺧﺖ و ﺳﺎز آﻣﯿﻨﻮاﺳﯿﺪﻫﺎي ﮔﻮﮔﺮدي اﺳﺖ. ﻫﺪف از اﯾﻦ ﻣﻄﺎﻟﻌﻪ، ﺑﻬﺒﻮد رﻧﮓﭘﺬﯾﺮي ﻣﺎﻫﯽ رزيﺑﺎرب ) Pethia conchonius( ﺑﻮد. ﺑﻪ اﯾﻦ ﻣﻨﻈﻮر ﺑﭽﻪﻣﺎﻫﯿﺎن )ﻣﯿﺎﻧﮕﯿﻦ وزن اوﻟﯿﻪ 7 ± 32 ﻣﯿﻠﯽﮔﺮم و ﻃﻮل 1/82± 12/83ﻣﯿﻠﯽﻣﺘﺮ( در ﭘﻨﺞ ﺗﯿﻤﺎر ﻣﺨﺘﻠﻒ ﺷﺎﻣﻞ ﮔﺮوه ﺷﺎﻫﺪ )ﺟﯿﺮه ﭘﺎ ﯾﻪ ﺑﺪون ﺗﻮرﯾﻦ و آﺳﺘﺎﮔﺰاﻧﺘﯿﻦ( و ﭼﻬﺎر ﺳﻄﺢ ﻣﺨﺘﻠﻒ ﺗﻮرﯾﻦ ﺷﺎﻣﻞ ﺻﻔﺮ، 100، 250و 500)ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﺟﯿﺮه( ﺑﻪ ﻫﻤﺮاه ﻣﯿﺰان ﺛﺎﺑﺖ آﺳﺘﺎﮔﺰاﻧﺘﯿﻦ ) 100ﻣﯿﻠﯽ ﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم( ﺑﻪ ﻣﺪت 60روز ﻣﻮرد ﺗﻐﺬﯾﻪ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﻧﺘﺎﯾﺞ ﺷﺎﺧﺺ-ﻫﺎي رﻧﮓﭘﺬﯾﺮي ﭘﻮﺳﺖ ﺑﻪ روش ﺗﺼﻮﯾﺮﺑﺮداري دﯾﺠﯿﺘﺎﻟﯽ و ﺳﻨﺠﺶ ﻣﺤﺘﻮاي ﮐﺎروﺗﻨﻮﺋﯿﺪ ﻋﻀﻠﻪ ﻧﺸﺎن داد ﮐﻪ ﺑﯿﺸﺘﺮﯾﻦ ﻣﯿﺰان رﻧﮓﭘﺬﯾﺮي در ﻣﺎﻫﯿﺎن ﺗﻐﺬﯾﻪ ﺷﺪه ﺑﺎ ﺟﯿﺮه ﺣﺎوي 100 + 250 )ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﺟﯿﺮه( ﺑﻪ ﺗﺮﺗﯿﺐ از آﺳﺘﺎﮔﺰاﻧﺘﯿﻦ و ﺗﻮرﯾﻦ ﻣﺸﺎﻫﺪه ﺷﺪ. اﻓﺰودن ﺗﻮرﯾﻦ ﺗﺎ ﺳﻄﺢ 250)ﻣﯿﻠﯽﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﺟﯿﺮه( ﻣﻨﺠﺮ ﺑﻪ اﻓﺰاﯾ ﺶ ﻣﯿﺰان ﭼﺮﺑﯽ ﮐﻞ، ﺗﺮيﮔﻠﯿﺴﺮﯾ ﺪ، ﮐﻠﺴﺘﺮول، LDLو HDLﻻﺷﻪ در ﻣﺎﻫﯿﺎن در ﻣﻘﺎﯾﺴﻪ ﺑﺎ ﮔﺮوه ﺷﺎﻫﺪ ﺷﺪ )0/05>p(. ﺑﻪ ﻧﻈﺮ ﻣﯽرﺳﺪ ﮐﻪ اﻓﺰودن ﺗﻮرﯾﻦ ﺑﻪ ﺟﯿﺮه ﺣﺎوي آﺳﺘﺎﮔﺰاﻧﺘﯿﻦ ﻣﯽﺗﻮاﻧﺪ ﺳﺒﺐ ﺗﺎﺛﯿﺮ ﺑﺮ ﻣﺘﺎﺑﻮﻟﯿﺴﻢ ﭼﺮﺑﯽ و درﻧﺘﯿﺠﻪ ﺑﻬﺒﻮد ﺟﺬب اﺳﺘﺎﮔﺰاﻧﺘﯿﻦ ﺟﯿﺮه و ﺷﺪت رﻧﮓﭘﺬﯾﺮي در ﻣﺎﻫﯽ رزيﺑﺎرب ﺷﻮد.

Taurine (2 amino ethane sulfonic acid), is a semi-essential amino acid and an end product of the sulfur amino acid metabolism. The aim of this study was improving Rosy barb (Pethia conchonius) pigmentation. The fish (average weight 32 ± 7 mg and length 12.83 ± 1.82 mm) were divided into five different groups including control (basic diet without taurine and astaxanthin) and four different levels of taurine (0, 100, 250 & 500 mg/kg diet) with constant amount of astaxanthin (100 mg/kg diet) and fed for 60 days. The pigmentation analysis was done using digital camera photography and whole body biochemical carotenoid analysis and showed that the fish fed on 100 + 250 mg/kg of astaxanthin & taurine respectively had the highest levels of pigmentation. Adding taurine up to 250 (mg/kg diet) caused increasing total carcasses lipid, plasma triglyceride, cholesterol, LDL & HDL levels in compression to the fish fed on control diet (p<0.05). It could be concluded that dietary taurine supplementation could affect fish lipid metabolism and improve astaxanthin absorption, which lead to better pigmentation in Rosy barb.