تاثير ويتامين هاي E، C و سلنيوم به عنوان محرك سيستم ايمني بر فاكتورهاي خوني، رشد و ايمني ماهي سي باس آسيايي (Lates calcarifer)

The effect of vitamins E, C and selenium as stimulants of the immune system on blood factors, growth and immunity of Asian cates (Lates calcarifer

اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﻪ ﻣﻨﻈﻮر ارزﯾﺎﺑﯽ ﺗﺎﺛﯿﺮ ﻣﺤﺮك ﺳﯿﺴﺘﻢ اﯾﻤﻨﯽ آﺑﺰﯾﺎن )وﯾﺘﺎﻣﯿﻦﻫﺎي C ،E و ﺳﻠﻨﯿﻮم( ﺑﺮ ﺷﺎﺧﺺﻫﺎي ﺧﻮﻧﯽ، ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ و اﯾﻤﻨﯽ ﺳﯽﺑﺎس آﺳﯿﺎﯾﯽ )Lates calcarifer( اﻧﺠﺎم ﮔﺮﻓﺖ. ﺑﻪ اﯾﻦ ﻣﻨﻈﻮر، 2 ﺗﯿﻤﺎر ﺷﺎﻫﺪ )ﻏﺬاي ﺑﺪون ﻣﺤﺮك ﺳﯿﺴﺘﻢ اﯾﻤﻨﯽ( و ﺗﯿﻤﺎر ﺑﺎ ﺟﯿﺮه ﻏﺬاﯾﯽ ﺣﺎوي وﯾﺘﺎﻣﯿﻦﻫﺎي C ،Eو ﺳﻠﻨﯿﻮم ﺑﻪ ﻣﯿﺰان 2% وزن ﺗﻮده زﻧﺪه ﺗﻐﺬﯾﻪ ﺷﺪﻧﺪ. در ﭘﺎﯾﺎن دوره، وزن ﻧﻬﺎﯾﯽ ﮔﺮوه ﺷﺎﻫﺪ 35/8 ± 761/3 ﮔﺮم و ﮔﺮوه ﺗﯿﻤﺎر 5/77 ± 850 ﮔﺮم و ﻧﺮخ رﺷﺪ وﯾﮋه ﮔﺮوهﻫﺎي ﺷﺎﻫﺪ و ﺗﯿﻤﺎر ﺑﻪ ﺗﺮﺗﯿﺐ 0/02 ± 1/3 و 0/01 ± 1/35 ﺑﻮد، وﻟﯽ ﺿﺮﯾﺐ ﺗﺒﺪﯾﻞ ﻏﺬاﯾﯽ اﺧﺘﻼف ﻣﻌﻨﯽداري ﻧﺸﺎن ﻧﺪاد. ﺗﻌﺪاد ﮔﻠﺒﻮلﻫﺎي ﻗﺮﻣﺰ و ﻣﻘﺪار ﻫﻤﻮﮔﻠﻮﺑﯿﻦ در ﺗﯿﻤﺎرﻫﺎي ﺣﺎوي ﻣﺤﺮكﻫﺎي اﯾﻤﻨﯽ و درﯾﺎﻓﺖﮐﻨﻨﺪه ﻣﮑﻤﻞ اﻓﺰاﯾﺸﯽ ﻣﻌﻨﯽداري را ﻧﺴﺒﺖ ﺑﻪ ﺷﺎﻫﺪ ﻧﺸﺎن دادﻧﺪ )0/05p(. ﮔﻠﺒﻮلﻫﺎي ﺳﻔﯿﺪ از 2964/84 ± 41417 در ﺗﯿﻤﺎر درﯾﺎﻓﺖ ﮐﻨﻨﺪه ﻣﮑﻤﻞ ﺗﺎ 683/13 ± 42500 در ﺗﯿﻤﺎر ﺷﺎﻫﺪ ﻣﺘﻐﯿﺮ ﺑﻮد و ﺗﻌﺪاد ﮔﻠﺒﻮلﻫﺎي ﻗﺮﻣﺰ در ﺗﯿﻤﺎر ﺷﺎﻫﺪ 191763/74 ± 2810000 و ﺗﯿﻤﺎر درﯾﺎﻓﺖ ﮐﻨﻨﺪه ﻣﮑﻤﻞ 14588/84 ± 3951667 ﺷﻤﺎرش ﮔﺮدﯾﺪ. ﺑﻨﺎﺑﺮاﯾﻦ ﻣﺸﺨﺺ ﺷﺪ ﮐﻪ اﯾﻦ ﻣﺤﺮك ﺳﯿﺴﺘﻢ اﯾﻤﻨﯽ ﺣﺎوي وﯾﺘﺎﻣﯿﻦﻫﺎي C ،Eو ﺳﻠﻨﯿﻮم ﻣﯽﺗﻮاﻧﺪ ﻧﻘﺸﯽ را در ﺑﻬﺒﻮد ﺷﺎﺧﺺﻫﺎي رﺷﺪ، ﺧﻮﻧﯽ، ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ و اﯾﻤﻨﯽ ﻣﺎﻫﯽ ﺳﯽﺑﺎس آﺳﯿﺎﯾﯽ ﭘﺮورﺷﯽ اﯾﻔﺎ ﻧﻤﺎﯾﺪ.

This study was performed to evaluate the effect of immune system stimulants (vitamins E, C and selenium) on biochemical and immunity indices of Late calcarifer. For this purpose, two treatments were selected. Treatment 1 or control group was fed with food without immune stimulant and treatment 2 with diet containing vitamins E, C and selenium at 2% of the biomass weight. Finally, growth efficiency indices were compared in two treatments. Results showed final weight of the control and treatment groups was 761.33 ± 35.8 g and 850.00 ± 5.77 g, respectively. The specific growth rate of the control and treatment groups was 1.3 ± 0.02 and 1.35 ± 0.01 but the feed conversion ratio did not show a significant difference. Hemoglobin and erythrocytes showed a significant increase compared to the control in treatments containing immune stimulants and supplementation (p<0.05). White blood cells ranged from 41417 ± 2964.84 in the treatment to 42500 ± 683.13 in the control treatment. The number of red blood cells in the control treatment was 2810000 ± 19.1763 and the recipient treatment was 3951667 ± 14588.84. Immune system stimulants including vitamins E, C and selenium can play a role in improving the growth, blood, biochemical and safety indices of farmed Late calcarifer.