اثر پودر زردچوبه (Curcuma longa) بر شاخص هاي خوني و بررسي هيستوپاتولوژيك كبد، كليه و روده در ماهي ازون برون (Acipenser stellatus)

The effect of turmeric (Curcuma longa) on blood parameters and histopathological study of liver, kidney and intestine in juvenile Stellate sturgeon (Acipenser stellatus

ﺗﺤﻘﯿﻖ ﺣﺎﺿﺮ ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﯽ اﻓﺰودن اﺛﺮ ﺳﻄﻮح ﻣﺨﺘﻠﻒ زردﭼﻮﺑﻪ در ﺟﯿﺮه ﺑﺮ ﺷﺎﺧﺺ ﻫﺎي ﺧﻮﻧﯽ و ﺑﺎﻓـﺖ ﻫﺎي ﮐﺒﺪ، ﮐﻠﯿﻪ و روده ﻣﺎﻫـ ﯽ ازونﺑﺮون ﺑﻪ ﻣﺪت 60روز اﺟـ ﺮا ﺷﺪ. ﺑﻪ اﯾﻦ ﻣـﻨﻈﻮر، 120 ﻋﺪد ﺑﭽﻪﻣﺎﻫﯽ ازونﺑﺮون )وزن 0/5± 45ﮔﺮم( ﺑﻪ ﺻﻮرت ﺗﺼﺎدﻓﯽ در 12اﺳﺘﺨﺮ ﺑﺘﻮﻧﯽ ﺗﻮزﯾﻊ ﺷﺪﻧﺪ. ﻣﺎﻫﯿﺎن ﺑﺎ 4 ﺟﯿﺮه آزﻣﺎﯾﺸﯽ ﻣﺤﺘﻮي ﺻﻔﺮ، 1 ،0/5 و 2% زردﭼﻮﺑﻪ ﺗﻐﺬﯾﻪ ﺷﺪﻧﺪ. ﺗﻐﺬﯾﻪ ﻣﺎﻫﯿﺎن 3 ﺑﺎر در روز و ﺑﻪ ﻣﯿﺰان 3% وزن ﺑﺪن اﻧﺠﺎم ﺷﺪ. در ﭘﺎﯾﺎن دوره ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﺗﯿﻤﺎر2 % زردﭼﻮﺑﻪ ﺑﺎﻋﺚ اﻓﺰاﯾﺶ ﻣﯿﺰان ﻫﻤﺎﺗﻮﮐﺮﯾﺖ، ﻫﻤﻮﮔﻠﻮﺑﯿﻦ، ﮔﻠﺒﻮل ﻗﺮﻣﺰ، ASTو ALTﺷﺪ و اﺧﺘﻼف ﺑﯿﻦ ﺗﯿﻤﺎرﻫﺎ ﻣﻌﻨﯽدار ﺑﻮد )0/05>p(. ﻫﻤﭽﻨﯿﻦ، ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﺑﺎ اﻓﺰاﯾﺶ ﻣﯿﺰان دوز در 2% زردﭼﻮﺑﻪ ﻣﯿﺰان WBCو آﻟﺒﻮﻣﯿﻦ اﻓﺰاﯾﺶ ﭘﯿﺪا ﮐﺮد ، اﻣﺎ اﺧـﺘﻼف ﻣﻌﻨﯽداري ﺑﯿﻦ ﺗﯿﻤﺎرﻫﺎ ﻣﺸﺎﻫﺪه ﻧﺸﺪ )0/05p(. ﻫﻤﭽﻨﯿﻦ، ﺑﺎ اﻓﺰاﯾﺶ د وز، ﻣﯿﺰان ﻟﻨﻔﻮﺳﯿﺖ و ﻧﻮﺗﺮوﻓﯿﻞ اﻓﺰاﯾﺶ و اﺋﻮزوﻧﻮﻓﯿﻞ ﮐﺎﻫﺶ ﯾﺎﻓﺖ، ﺑﻪ ﻃﻮري ﮐﻪ ﺑﯿﺸﺘﺮﯾﻦ ﻣﯿﺰان آنﻫﺎ ﻣﺮﺑﻮط ﺑﻪ ﺗﯿﻤﺎر 3ﺑﻮد، اﻣﺎ اﺧـﺘﻼف ﺑﯿﻦ ﺗﯿﻤﺎرﻫﺎ ﻣﻌﻨﯽدار ﻧﺒﻮد )0/05

The aim of this study is to evaluate the effect of different levels of dietary turmeric on blood indices and histopathological examination of liver, kidney and intestine in juvenile Stellate sturgeon. So, 120 fish (45 ± 0.5 g in weight) were randomly distributed in 12 concrete tanks. The fish were fed with 4 experimental diets containing different levels including 0, 0.5, 1 and 2% of turmeric-added formulated diet. At the end of the experiment, blood indices and histopathological examination of liver, kidney and intestine were evaluated. Increased dose of turmeric (2%) elevated the hematocrit, hemoglobin, RBC, AST, ALT (p<0.05). The results also showed that by elevated dose (2%) of turmeric, the amount of WBC and Alb did not exhibit significant differences between the treatments (p>0.05). The results showed that the MCV, MCH and MCHC levels significantly decreased by upraising the dose of turmeric (p<0.05). In histological examinations of liver, kidney and intestine, hepatocytes did not display any cellular disturbances. In addition, no specific histological alterations were observed in the intestine of all treatments. The comparison of mean lymphocytes, neutrophils, monocytes, and eosinophils in different treatments revealed that the number of lymphocytes and neutrophils upraised, while eosinophils dropped. Based on the results of this study, adding turmeric powder to the diet at the level of 2% is recommended.