عنوان مقاله :
مقايسه اثر استفاده از باكتري كشته شده آئروموناس هيدروفيلا به سه روش حرارت، فرمالين و اشعه ماوراء‌بنفش بر ميزان بقاء، برخي از فاكتورهاي خوني و ايمني در ماهي قزل‌آلاي رنگين‌كمان (Oncorhynchus mykiss)
عنوان به زبان ديگر :
Comparison of the effect of using killed Aeromonas hydrophila bacteria by three methods: heat, formalin and ultraviolet light on survival rate and immune factors in rainbow trout (Oncorhynchus mykiss
پديد آورندگان :
محمدي فوتمي، تقي داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ ﺑﺎﺑﻞ - ﮔﺮوه ﺷﯿﻼت , چنگيزي، رضا داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ ﺑﺎﺑﻞ - ﮔﺮوه ﺷﯿﻼت , حسيني فرد، مهدي داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ ﺑﺎﺑﻞ - ﮔﺮوه دامپزشكي , خارا، حسين داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ لاهيجان - ﮔﺮوه ﺷﯿﻼت , صفري، رضا ﺳﺎزﻣﺎن ﺗﺤﻘﯿﻘﺎت آﻣﻮزش و ﺗﺮوﯾﺞ ﮐﺸﺎورزي ﺳﺎري - ﭘﮋوﻫﺸﮑﺪه اﮐﻮﻟﻮژي درﯾﺎي ﺧﺰر - ﮔﺮوه ﺗﻐﺬﯾﻪ
كليدواژه :
آﺋﺮوﻣﻮﻧﺎس ﻫﯿﺪروﻓﯿﻼ , ﻗﺰل آﻻي رﻧﮕﯿﻦ ﮐﻤﺎن , ايمني , اﯾﻤﻨﻮﮔﻠﻮﺑﯿﻦ
چكيده فارسي :
آﺋﺮوﻣﻮﻧﺎس ﻫﯿﺪروﻓﯿﻼ ﯾﮑﯽ از ﺑﺎﮐﺘﺮي ﻫﺎي ﻣﻬﻢ و ﺷﺎﯾﻊ در اﻣﺮ ﭘﺮورش ﻣﺎﻫﯿﺎن ﻣﯽﺑﺎﺷﺪ ﮐﻪ ﻣﺘﺎﺳﻔﺎﻧﻪ ﺷﯿﻮع و ﮔﺴﺘﺮش آن ﻣﻨﺠﺮ ﺑﻪ اﻓﺰاﯾﺶ ﻣﺼﺮف آﻧﺘﯽ ﺑﯿﻮﺗﯿﮏ در ﺗﻮﻟﯿﺪ آﺑﺰﯾﺎن ﺷﺪه اﺳﺖ. ﻟﺬا در ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ ﺑﻪ ﻣﻨﻈﻮر اراﺋﻪ راﺣﻞ و اﻓﺰاﯾﺶ اﯾﻤﻨﯽ در ﻣﺎﻫﯽ ﻗﺰل آﻻي رﻧﮕﯿﻦ ﮐﻤﺎن، ﺑﻪ 4 ﮔﺮوه 20 ﺗﺎﯾﯽ از ﻣﺎﻫﯿﺎن ﺑﺎ 3 ﺗﮑﺮار ﺑﺮاي ﻫﺮ ﮔﺮوه ﺑﺎﮐﺘﺮي زﻧﺪه و ﺑﺎﮐﺘﺮي ﮐﺸﺘﻪ ﺷﺪه آﺋﺮوﻣﻮﻧﺎس ﻫﯿﺪروﻓﯿﻼ ﺑﻪ ﺳﻪ روش ﺣﺮارت، ﻓﺮﻣﺎﻟﯿﻦ و UV ﺑﺼﻮرت ﺗﺰرﯾﻖ درون ﺻﻔﺎﻗﯽ ﺑﺎ ﻏﻠﻈﺖ 10 ﺑﻪ ﻧﻤﺎي 5 ﺳﻠﻮل در ﻣﯿﻠﯽ ﻟﯿﺘﺮ ﺑﻪ ﻣﺎﻫﯽ ﻣﻌﺮﻓﯽ ﺷﺪ. ﺑﺮاﺳﺎس ﻧﺘﺎﯾﺞ، آﻧﺘﯽ ژن ﺑﺎﮐﺘﺮي ﮐﺸﺘﻪ ﺷﺪه ﺑﻪ روش ﻓﺮﻣﺎﻟﯿﻦ و UV ﻣﻮﺟﺐ اﻓﺰاﯾﺶ ﮔﻠﺒﻮل ﻫﺎي ﺳﻔﯿﺪ )0/05˂P و ﺣﻔﻆ ﮔﻠﺒﻮل ﻫﺎي ﻗﺮﻣﺰ ﺷﺪه اﺳﺖ. ﻫﻤﭽﻨﯿﻦ ﺳﻄﺢ اﯾﻤﻨﻮﮔﻠﻮﺑﯿﻦ ﻧﻮع M و ﮐﻤﭙﻠﻤﺎن 3و 4 در اﯾﻦ دو ﮔﺮوه ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوﻫﯽ ﮐﻪ در ﻣﻌﺮض ﺑﺎﮐﺘﺮي زﻧﺪه و ﺑﺎﮐﺘﺮي ﮐﺸﺘﻪ ﺷﺪه ﺑﻪ روش ﺣﺮارت ﻗﺮار داﺷﺘﻨﺪ، ﺷﺮاﯾﻂ ﺑﻬﺘﺮي داﺷﺖ. ﻟﺬا ﺑﺮاﺳﺎس ﻧﺘﺎﯾﺞ ﺑﺎ اﺳﺘﻔﺎده از ﺑﺎﮐﺘﺮيﻫﺎي ﮐﺸﺘﻪ ﺷﺪه آﺋﺮوﻣﻮﻧﺎس ﻫﯿﺪرو ﻓﯿﻼ ﺑﻪ روش ﻓﺮﻣﺎﻟﯿﻦ و UV ﻣﯽﺗﻮان اﻧﺘﻈﺎر اﻓﺰاﯾﺶ اﯾﻤﻨﯽ ﻣﺎﻫﯽ راد داشت.
چكيده لاتين :
Aeromonas hydrophila is one of the most important bacteria in fish farming, which unfortunately has led to an increase in the use of antibiotics in aquaculture. Therefore, in the present study, in order to provide comfort and increase immunity in rainbow trout, we introduced live bacteria and bacteria killed Aeromonas hydrophila by three methods of heat, formalin and UV as intraperitoneal injection with a concentration of 105 to 4 groups of fish with 3 repetitions for each group. According to the results, the bacterial antigen killed by formalin and UV increased white blood cells (P˂0.05) and retained red blood cells. Also, the levels of Igm and complement 3 and 4 in these two groups were better than those in the group exposed to live bacteria and bacteria killed by heat. Total protein was higher in a group of fish exposed to bacteria killed by formalin and UV but albumin levels did not change significantly. Therefore, based on the results; it is possible to expect an increase in fish immunity by using killed Aeromonas hydrophila bacteria by formalin and UV methods.
عنوان نشريه :
توسعه آبزي پروري
