مقايسه‌ تاثير ادجوانت مونتانايد (Montanide™ ISA 763 AVG) و عصاره شيرين بيان (Glycyrrhiza glabra) بر شاخص‌هاي رشد و بازماندگي ماهي قزل‌‌ آلاي رنگين‌كمان (Oncorhynchus mykiss) واكسينه شده با واكسن لاكتوكوكوزيس

Comparison of adjuvant (Montanide™ ISA 763 AVG) and licorice extract (Glycyrrhiza glabra) impacts on the growth performances and survival of rainbow trout vaccinated with lactococcosis vaccine

هدف از اين مطالعه مقايسه‌ تأثير ادجوانت مونتانايد (Montanide™ ISA 763 AVG) و عصاره شيرين بيان (Glycyrrhiza glabra)ﺑﺮ ﺷﺎﺧﺺﻫﺎي رﺷﺪ و ﻣﯿﺰان ﺑﺎزﻣﺎﻧﺪﮔﯽ ﻣﺎﻫﯽ ﻗﺰلآﻻي رﻧﮕﯿﻦﮐﻤﺎن واﮐﺴﯿﻨﻪ ﺷﺪه ﺑﺎ واﮐﺴﻦ ﻻﮐﺘﻮﮐﻮﮐﻮزﯾﺲ ﺑﻮد. ﺑﻪ اﯾﻦ ﻣﻨﻈﻮر ﻣﺎﻫﯿﺎن ﻗﺰلآﻻي رﻧﮕﯿﻦﮐﻤﺎن ﺑﺎ ﻣﯿﺎﻧﮕﯿﻦ وزن اوﻟﯿﻪ 72±3 ﮔﺮم در ﭼﻬﺎر ﺗﯿﻤﺎر آزﻣﺎﯾﺸﯽ ﺷﺎﻣﻞ ﺷﺎﻫﺪ، واﮐﺴﻦ، واﮐﺴﻦ ﻫﻤﺮاه ﺑﺎ ﮐﻤﮏ اﯾﻤﻨﯽ ﻣﻮﻧﺘﺎﻧﺎﯾﺪ و واﮐﺴﻦ ﻫﻤﺮاه ﺑﺎ ﻋﺼﺎره ﺷﯿﺮﯾﻦ ﺑﯿﺎن ﺗﻘﺴﯿﻢ ﺷﺪﻧﺪ. ﻫﺮ ﺗﯿﻤﺎر ﺣﺎوي 60 ﻗﻄﻌﻪ ﻣﺎﻫﯽ در 3 ﺗﮑﺮار در 12 ﻣﺨﺰن 1000 ﻟﯿﺘﺮي در ﯾﮏ ﺳﯿﺴﺘﻢ ﭘﺮورش ﻣﺪار ﺑﺴﺘﻪ ﻃﺮاﺣﯽ ﺷﺪ. ﭘﺲ از ﺳﺎزﮔﺎري ﺑﺎ ﺷﺮاﯾﻂ ﺟﺪﯾﺪ اﺑﺘﺪا ﻣﺎﻫﯿﺎن ﺑﺮاي 24 ﺳﺎﻋﺖ ﻗﻄﻊ ﻏﺬا و ﭘﺲ از ﺑﯿﻬﻮﺷﯽ ﺑﺎ اﺳﺎﻧﺲ ﮔﻞ ﻣﯿﺨﮏ ﺑﻪ روش داﺧﻞ ﺻﻔﺎﻗﯽ واﮐﺴﻦ ﯾﺎ واﮐﺴﻦ ﺣﺎوي ﮐﻤﮏ اﯾﻤﻨﯽ و ﻋﺼﺎره ﮔﯿﺎﻫﯽ درﯾﺎﻓﺖ ﻧﻤﻮدﻧﺪ. ﺳﭙﺲ ﻣﺎﻫﯿﺎن ﺑﺮاي 8 ﻫﻔﺘﻪ ﻣﻮرد ﺗﻐﺬﯾﻪ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. در ﭘﺎﯾﺎن دوره ﺑﺎ اﯾﺠﺎد ﺑﯿﻬﻮﺷﯽ و ﺑﯿﻮﻣﺘﺮي ﺷﺎﺧﺺﻫﺎي رﺷﺪ ﻣﻮرد آﻧﺎﻟﯿﺰ ﻗﺮار ﮔﺮﻓﺖ و ﻣﯿﺰان ﺑﻘﺎ ﻧﯿﺰ ﺗﻌﯿﯿﻦ ﺷﺪ. ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﺑﯿﺸﺘﺮﯾﻦ اﻓﺰاﯾﺶ وزن، ﻧﺮخ رﺷﺪ وﯾﮋه، رﺷﺪ روزاﻧﻪ، ﻧﺴﺒﺖ ﮐﺎراﯾﯽ ﺗﺒﺪﯾﻞ ﻏﺬا، ﭘﺮوﺗﺌﯿﻦ، ﭼﺮﺑﯽ و ﮐﺎﻫﺶ ﺿﺮﯾﺐ ﺗﺒﺪﯾﻞ ﻏﺬا ﺑﻪﺗﺮﺗﯿﺐ در ﺗﯿﻤﺎرﻫﺎي واﮐﺴﻦ ﺣﺎوي ﻣﻮﻧﺘﺎﻧﺎﯾﺪ، واﮐﺴﻦ ﺣﺎوي ﻋﺼﺎره و واﮐﺴﻦ ﺑﻪدﺳﺖ آﻣﺪ )0/05

The aim of this study was Comparison of adjuvant (Montanide™ ISA 763 AVG) and licorice extract (Glycyrrhiza glabra) impacts on the growth performances and survival of rainbow trout vaccinated with lactococcosis vaccine. For this purpose, rainbow trout with an average initial weight of 72±3 g with four experimental treatments including control, vaccine, vaccine with Montanid safety vaccine and vaccine with licorice extract and each treatment containing 30 fish in 3 repetitions in 12 tanks 1,000liter was divided into a closed-circuit breeding system, and after adapting to the new conditions, the midwives first received the vaccine for 24 hours and after anesthesia with clove essential oil by intraperitoneal vaccine or vaccine containing immunosuppression and herbal extract. The fish were then fed for 8 weeks with extruded food faradaeneh and according to the factory's instructions. At the end of the period, growth indicators were analyzed by anesthesia and biometrics and the survival rate was determined. The highest final weight was (102.07±2.49 g and 100.47±3.27 g), respectively, for vaccine-containing montanide and vaccine-containing licorice extracts (P<0.05), respectively. Also, the highest percentage of survival in the vaccine group contained Montanid immunosuppression (96.66±3.33%) and the lowest rate in the control group (20±2%). The results of this study showed that the addition of Montanid's safety aid and licorice extract did not have a negative effect on salivary growth index, while improving some of these characteristics.