تغييرات اسيد‌هاي چرب در مرحله جنيني و لاروي ماهي صبيتي Sparidentex hasta

Fatty acid changes in embryonic and larval stages of (Sparidentex hasta

ﺑﺎ ﺗﻮﺳﻌﻪ آﺑﺰيﭘﺮوري در ﺑﺨﺶ ﻣﺎﻫﯿﺎن درﯾﺎﯾﯽ، ﺷﻨﺎﺳﺎﯾﯽ ﺗﺮﮐﯿﺐ ﻣﻮاد ﻣﻐﺬي ﻣﻮردﻧﯿﺎز ﻻرو در ﻣﺮاﺣﻞ آﻏﺎزﯾﻦ زﻧﺪﮔﯽ، ﺑﯿﺸﺘﺮ ﻣﻮرد ﺗﻮﺟﻪ ﻗﺮار ﮔﺮﻓﺘﻪاﺳﺖ. ﻋﻠﯽرﻏﻢ اﻫﻤﯿﺖ آﺷﮑﺎر ﺗﻐﺬﯾﻪ در رﺷﺪ ﻻرو ﻣﺎﻫﯽ ﺻﺒﯿﺘﯽ، ﮐﻪ از ﮔﻮﻧﻪﻫﺎي اﻗﺘﺼﺎدي در آﺑﺰيﭘﺮوري ﺟﻨﻮب ﮐﺸﻮر اﺳﺖ، ﺑﻪ-دﻟﯿﻞ رﯾﺰ و ﺣﺴﺎس ﺑﻮدن ﺑﻪ دﺳﺘﮑﺎري ﻻرو ﻣﺎﻫﯿﺎن درﯾﺎﯾﯽ، اﻃﻼﻋﺎت ﮐﻤﯽ در ﻣﻮرد اﺣﺘﯿﺎﺟﺎت ﻏﺬاﯾﯽ در دﺳﺖ ﻣﯽﺑﺎﺷﺪ. ﺗﺮﮐﯿﺒﺎت ﺟﻨﯿﻦ و ﮐﯿﺴﻪ زرده ﺗﺤﺖ ﺗﺎﺛﯿﺮ ﺗﻐﺪﯾﻪ ﻣﻮﻟﺪﯾﻦ ﻣﯽﺑﺎﺷﺪ و ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ ﻧﻘﺶ ﺣﯿﺎﺗﯽ اﺳﯿﺪﻫﺎي ﭼﺮب در ﻧﻔﻮذﭘﺬﯾﺮي ﻏﺸﺎ در ﻣﺮﺣﻠﻪ ﻻروي، ﻣﯽﺗﻮان ﺑﺎ ﻣﺪﯾﺮﯾﺖ ﺻﺤﯿﺢ ﺗﺮﮐﯿﺒﺎت اﺳﯿﺪﻫﺎي ﭼﺮب ﻣﻮردﻧﯿﺎز در ﺷﺮوع ﺗﻐﺪﯾﻪ آﻏﺎزﯾﻦ )ﻏﺬاي ﺧﺸﮏ و ﯾﺎ ﻏﻨﯽ ﺳﺎزي ﻏﺬاي زﻧﺪه(، درﺻﺪ ﺑﻘﺎي ﻻروﻫﺎ را اﻓﺰاﯾﺶ داد. در اﯾﻦ ﺗﺤﻘﯿﻖ ﻧﻤﻮﻧﻪﻫﺎ در ﺳﻪ ﻣﺮﺣﻠﻪ ﮔﺎﺳﺘﺮوﻻ، در زﻣﺎن ﺟﺬب ﮐﯿﺴﻪ زرده و ﺳﻪ روز ﭘﺲ از ﺟﺬب ﮐﺎﻣﻞ ﮐﯿﺴﻪ زرده )آﻏﺎز ﺗﻐﺪﯾﻪ ﻓﻌﺎل( ﺑﺮداﺷﺖ ﮔﺮدﯾﺪ. ﺳﭙﺲ داﺧﻞ ازت ﻣﺎﯾﻊ و ﻓﺮﯾﺰر 80- درﺟﻪ ﺗﺎ زﻣﺎن اﻧﺠﺎم ﮐﺎر آزﻣﺎﯾﺸﮕﺎﻫﯽ ﺑﺎ اﺳﺘﻔﺎده از ﮐﺮوﻣﺎﺗﻮﮔﺮاﻓﯽ ﮔﺎزي GC، ﻧﮕﻬﺪاري ﺷﺪ. ﻓﺮاوانﺗﺮﯾﻦ اﺳﯿﺪﻫﺎي ﭼﺮب، اوﻟﺌﯿﮏ و ﭘﺎﻟﻤﺘﯿﮏ ﺑﻮد و در ﻣﺮاﺣﻞ ﻣﺨﺘﻠﻒ اﺳﯿﺪﻫﺎي ﭼﺮب اﺧﺘﻼف ﻣﻌﻨﯽدار آﻣﺎري ﻣﺸﺎﻫﺪه ﮔﺮدﯾﺪ )05.P<0(. ﺑﺎﺗﻮﺟﻪ ﺑﻪ ﻧﺘﺎﯾﺞ درPUFA SFA ﺗﺎ ﻣﺮﺣﻠﻪ ﻻروي روﻧﺪ اﻓﺰاﯾﺸﯽ و در MUFA و اوﻟﺌﯿﮏ روﻧﺪ ﮐﺎﻫﺸﯽ ﺑﻮد. ﮐﺎﻫﺶ ﺑﺮﺧﯽ اﺳﯿﺪ-ﻫﺎي ﭼﺮب ﺑﻪدﻟﯿﻞ ﻣﺼﺮف اﻧﺮژي و اﻓﺰاﯾﺶ ﺑﯿﺸﺘﺮ ﺑﺮاي ﻧﻘﺶ ذﺧﯿﺮه ﺳﺎزي ﻣﺨﺼﻮﺻﺎ در ﺑﺎﻓﺖ ﻏﺸﺎﯾﯽ اﺳﺖ. ﺑﻨﺎﺑﺮاﯾﻦ ﻣﻘﺪار و ﻧﺴﺒﺖ اﺳﯿﺪﻫﺎي ﭼﺮب در ﻣﺮاﺣﻞ اوﻟﯿﻪ زﻧﺪﮔﯽ ﻣﺎﻫﯽ ﺻﺒﯿﺘﯽ، ﺣﺪاﻗﻞ ﻧﯿﺎز ﻻروي در ﺟﯿﺮه آﻏﺎزﯾﻦ را ﺗﻌﯿﯿﻦ ﻣﯽﮐﻨﺪ.

With the development of aquaculture in the marine fish sector, the identification of the nutrient composition required by larvae in the early stages of life has more considered. Despite the obvious importance of nutrition in the development of larvae of Sparidentex hasta, which is an economic species in aquaculture at; the south of the country, due to the small size and sensitivity to manipulation of marine fish larvae, little information is available about nutritional needs. Embryo and yolk sac compounds are affected by reproductive nutrition and according to the vital role of fatty acids in membrane permeability in the larval stage; it is possible to increase the survival rate of larvae by proper management of the fatty acid compounds required at the beginning of initial feeding (dry food or live food enrichment). In this study, samples were taken in three stages of gastrula, at the time of yolk sac uptake and three days after complete yolk sac uptake (beginning of active feeding). It was then stored in liquid nitrogen and -80 ° C freezer until laboratory work was performed using GC gas chromatography. The most abundant fatty acids were oleic and palmitic and there was a statistically significant difference in different stages of fatty acids (P<0.05). According to the results in SFA and PUFA up to larval stage, the trend was increasing, and in MUFA, oleic, it was a decreasing trend. The reduction of some fatty acids is because of energy consumption and further increase is for the role of storage, especially in membrane tissue. Therefore, the amount and ratio of fatty acids in the early stages of the life of the Sparidentex hasta determines the minimum larval requirement in the initial diet.