عنوان به زبان ديگر :
Determination and Comparison of Total Phenolic Content and Antioxidant Activity of Water, Methanolic, Ethanolic and Hexane Extracts from Different Parts of Perovskia abrotanoides Karel.
پديد آورندگان :
ﺑﯿﺎت، ﺣﺴﻦ داﻧﺸﮕﺎه ﺑﯿﺮﺟﻨﺪ - داﻧﺸﮑﺪه ﮐﺸﺎورزي - ﮔﺮوه ﻋﻠﻮم ﺑﺎﻏﺒﺎﻧﯽ , ﻋﻠﯿﺮﺿﺎﯾﯽ ﻧﻘﻨﺪر، ﻣﺮﺗﻀﯽ داﻧﺸﮕﺎه ﻓﺮدوﺳﯽ ﻣﺸﻬﺪ - داﻧﺸﮑﺪه ﮐﺸﺎورزي , اﻣﯿﻨﯽ ﻓﺮد، ﻣﺤﻤﺪ ﺣﺴﯿﻦ داﻧﺸﮕﺎه ﺑﯿﺮﺟﻨﺪ - داﻧﺸﮑﺪه ﮐﺸﺎورزي - ﮔﺮوه ﻋﻠﻮم ﺑﺎﻏﺒﺎﻧﯽ
كليدواژه :
ﺑﺮازﻣﺒﻞ , ﺗﺮﮐﯿﺒﺎت ﻓﻨﻠﯽ , ﻋﺼﺎرهﻫﺎي ﮔﯿﺎﻫﯽ , ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ
چكيده فارسي :
ﭼﮑﯿﺪه ﺑﺮازﻣﺒﻞ )Perovskia abrotanoides Karel.( ﯾﮑﯽ از ﮔﯿﺎﻫﺎن ﺑﻮﻣﯽ ﺑﺎارزش داروﯾﯽ و داراي ﻣﻘﺎدﯾﺮ ﻗﺎﺑﻞ ﺗﻮﺟﻬﯽ از ﺗﺮﮐﯿﺒﺎت آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ اﺳﺖ ﮐﻪ ﺑﻪ ﻋﻠﺖ ﺧﻮاص ﺿﺪ ﻣﯿﮑﺮوﺑﯽ، ﮐﺎرﺑﺮد زﯾﺎدي در ﺑﯿﻦ اﻫﺎﻟﯽ ﺑﻮﻣﯽ ﺷﻤﺎل ﺷﺮق اﯾﺮان دارد. ﻫﺪف اﯾﻦ ﭘﮋوﻫﺶ اﻧﺪازه ﮔﯿﺮي و ﻣﻘﺎﯾﺴﻪ ﺗﺮﮐﯿﺒﺎت ﻓﻨﻠﯽ و ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ در ﻋﺼﺎرهﻫﺎي ﺣﺎﺻﻞ از ﺣﻼلﻫﺎي )ﻣﺘﺎﻧﻮل، اﺗﺎﻧﻮل، ﻫﮕﺰان و آب(، ﻏﻠﻈﺖﻫﺎي )0، 31/25، 62/5، 125، 250 و 500 ﭘﯽ ﭘﯽ ام( و اﻧﺪامﻫﺎي ﻣﺨﺘﻠﻒ )ﮔﻞ، ﺳﺎﻗﻪ و ﺑﺮگ( ﮔﯿﺎه ﺑﺮازﻣﺒﻞ ﺑﻮد. ﺑﺪﯾﻦ ﻣﻨﻈﻮر اﻧﺪام ﻫﻮاﯾﯽ ﮔﯿﺎﻫﺎن در ﻣﺮﺣﻠﻪي اوج ﮔﻠﺪﻫﯽ از روﯾﺸﮕﺎه ﺟﻤﻊ آوري و ﺑﻪ ﺳﻪ ﺑﺨﺶ ﮔﻞ، ﺳﺎﻗﻪ و ﺑﺮگ ﺗﻔﮑﯿﮏ ﺷﺪﻧﺪ. ﻧﺘﺎﯾﺞ ﺣﺎﺻﻠﻪ از ﺳﻨﺠﺶ ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ ﻧﺸﺎن داد ﮐﻪ در ﻣﯿﺎن ﻋﺼﺎرهﻫﺎي ﻣﺨﺘﻠﻒ ﺑﯿﺸﺘﺮﯾﻦ و ﮐﻤﺘﺮﯾﻦ ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ و ﺗﺮﮐﯿﺒﺎت ﻓﻨﻠﯽ ﺑﻪ ﺗﺮﺗﯿﺐ ﻣﺮﺑﻮط ﺑﻪ ﻋﺼﺎرهﻫﺎي ﻣﺘﺎﻧﻮﻟﯽ و ﻫﮕﺰاﻧﯽ اﺳﺖ. در ﺗﻤﺎﻣﯽ ﺣﻼلﻫﺎي ﻣﻮرد اﺳﺘﻔﺎده، ﺑﺎ اﻓﺰاﯾﺶ ﻏﻠﻈﺖ ﻋﺼﺎره از 31/25 ﺗﺎ 250 ﭘﯽ ﭘﯽ ام، ﻣﯿﺰان ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ ﻧﯿﺰ اﻓﺰاﯾﺶ ﯾﺎﻓﺖ و ﺑﺎ اﻓﺰاﯾﺶ ﻏﻠﻈﺖ از 250 ﺑﻪ 500 ﭘﯽ ﭘﯽ ام، ﮐﺎﻫﺶ ﺟﺰﺋﯽ در ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ ﻣﺸﺎﻫﺪه ﺷﺪ. ﻫﻤﭽﻨﯿﻦ ﻣﻘﺪار ﺗﺮﮐﯿﺒﺎت ﻓﻨﻠﯽ و ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ در ﻋﺼﺎرهﻫﺎي ﮔﻞ ﺑﯿﺸﺘﺮﯾﻦ و در ﻋﺼﺎرهﻫﺎي ﺳﺎﻗﻪ ﮐﻤﺘﺮﯾﻦ ﺑﻮد. ﺳﻨﺠﺶ فاكتور IC50 در ميان عصاره‌هاي مختلف و مقايسه آن با محلول حاوي استاندارد آسكوربيك اسيد حاكي از آن بود كه بيشترين اثر بازدارندگي بعد از آسكوربيك اسيد، در عصاره‌هاي متانولي حاصل شد. بطور كلي نتايج نشان داد كه گياه برازمبل و مخصوصا گل‌‌ها غني از تركيبات فنلي مي‌‌باشد و متانول به عنوان بهترين حلال جهت استخراج تركيبات فنلي و آنتي‌اكسيداني مي‌‌باشد.
چكيده لاتين :
Perovskia abrotanoides Karel. is one of native medicinally valuable plant, which is important for
the presence of significant amount of antioxidant compounds, used for antimicrobial effects
among indigenous people in Northeast of Iran. The objective of this study was to investigate the
antioxidant activities (AA) and total phenolic content (TPC) and IC50 (by using concentrations of
31.25, 62.5, 125, 250 and 500 ppm, as compared to the standard ascorbic acid) changes in
different extraction solvents (methanol, ethanol, hexane and water), extract concentration and
plant parts (flower, shoot and leaf) of P. abrotanoides Karel. The wild-growing plants were
harvested at full flowering stage, in October 2016, dissected into flower, shoot and leaf tissues,
which were dried. Dried and powdered plant materials were extracted by maceration using
different solvents. The results showed, that the greatest and the lowest of TPC and AA was
obtained in methanolic extract and hexane extract, respectively. Among the different organs, the
highest and the lowest TPC and AA were obtained in flower and shoot, respectively. In all
solvent extraction, with increasing of concentration from 31.25 to 250 ppm, AA was increased,
but showed a slight decrease in the concentration of 500 ppm. The lowest IC50 was observed in
ascorbic acid followed by methanolic extract and the highest was obtained in hexane extract.
Among different plant parts, the lowest and the highest IC50 were observed in flower and shoot,
respectively. It is concluded that P. abrotanoides Karel is a rich source of phenolic compounds.
Methanol evaluated as the best solvent for extraction of antioxidant and phenolic compounds.
