مطالعه تاثير تابش ليزر كم توان به همراه ويتامين A بر زنده مانايي و اپپتوز سلول هاي ملانوماي پوستي A375

Effect of low level laser irradiation with vitamin A on cell viability and apoptosis induction of human skin melanoma

سرطان پوست از نظر ابتلا در بين همه تومورها شايع تر بوده است. ملانوم نوع كمتر شايعي از سرطان پوست و كشنده ترين سرطان پوست است. مصرف ويتامين A در پيشگيري و درمان سرطان پوست توصيه مي شود. ليزر به عنوان ابزاري براي درمان بسياري از ضايعات پوستي استفاده مي شود. در اين مطالعه تاثير تابش ليزر كم توان به همراه ويتامين A بر فاكتورهاي سلولي سلول هاي ملانوماي پوستي مورد بررسي قرار گرفت. روش بررسي مطالعه آزمايشگاهي مداخله اي و در محيط In vitro آزمايشگاه كشت سلولي سازمان جهاد دانشگاهي علوم پزشكي تهران از تير 1399 تا تير 1400 انجام شد. پس از تهيه و كشت سلول هاي رده A375، تيمارهاي ويتامين A با غلظت هاي مختلف و ليزر كم توان با دوزهاي انرژي مختلف بر روي سلول ها انجام شد. مطالعه همزمان اين تيمار ها نيز در از بين بردن سلول هاي سرطاني ملانوم پوستي انجام شد. با روش تست MTT و فلوسايتومتري به ترتيب ميزان زنده مانايي و اپپتوز در اثر اين تيمارها بررسي شد. يافته ها نتايج نشان داد كه ليزر كم توان با دوزهاي دو و J/cm2 5 و ويتامين A با غلظت µmol 50 كمترين زنده مانايي و بيشترين القاي اپپتوز را سبب مي شود (0/01

Skin cancer is the most prevalent type of cancer and melanoma is the deadliest kind of skin cancer in the world. Due to enhanced induction of apoptosis and ROS levels, low-level lasers can be utilized to destroy skin cancer cells. Lasers are used to treat some skin lesions. Vitamin A is beneficial in the prevention and treatment of skin cancer. Vitamin A inhibits the pathway of cancer signals in the skin and suppresses tumor growth. In this study, the combined effect of low-level laser radiation (LLL) and vitamin A on cellular factors of skin melanoma cancer cells was investigated. Methods An in-vitro interventional laboratory study was performed in the cell culture laboratory of Medical Laser Research Center, Yara Institute in 2020-2021 (July 2020 to July 2021). First, A375 skin cancer cells were cultured in DMEM with 10% FBS. After preparation and culture of A375 cell lines, different concentrations of vitamin A (1, 5, 50, 100 μM) and LLL energy doses (1, 2, 5, 10 J/cm2) as treatments were done. Combination research of these treatments was performed to eliminate skin melanoma cancer cells. The rate of viability was determined using the MTT test, and the rate of apoptosis was determined using flow cytometry. Results The results indicated that a low-level laser with energy dosages of two and 5 J/cm2 and vitamin A treatment with a concentration of 50 μM in the A375 skin cancer cell line had the lowest viability and the highest induction of apoptosis. Furthermore, the results of the combination of Vitamin A and LLL treatments showed a synergistic effect with a greater reduction in the viability of skin melanoma cells and a greater amount of apoptosis. Conclusion In general, vitamin A and Low-level laser diminish the viability of cancer cells. Combination therapy of Low-level laser in the effective dose with vitamin A in optimal concentration provides anti-cancer effects. Further reductions in cancer cell viability caused by vitamin A and low-level laser radiation could pave the way for a novel approach in cancer treatment.