بررسي ميكروسكوپي اجسام بالبياني (Balbiani body) در ماهي شانك زردباله (Acanthopagrus latus)، ماهي بياح (Planiliza abu)، ماهي كپور معمولي (Cyprinus carpio) و ماهي بني (Barbus sharpeyi)

Microscopic survey of Balbiani bodies in Yellowfin seabream (Acanthopagrus latus), Abu Mullet (Planiliza abu), Common carp (Cyprinus carpio) and Benni (Barbus sharpeyi)

اﺟﺴﺎم ﺑﺎﻟﺒﻴﺎﻧﻲ ﺑﻪ ﻋﻨﻮان ﺳﺎﺧﺘﺎرﻫﺎﻳﻲ ﺟﻬﺖ ﺗﻌﻴﻴﻦ ﺳﺮﻧﻮﺷﺖ ﺳﻠﻮلﻫﺎي زاﻳﺎ، ﺷﻜﻞﮔﻴﺮي اﻧﺪاﻣﻚ ﻫﺎ و ﻗﺮارﮔﻴﺮي ﻧﻬﺎﻳﻲ آﻧﻬﺎ در ﺳﻴﺘﻮﭘﻼﺳﻢ اووﺳﻴﺖ ﺷﻨﺎﺧﺘﻪ ﺷﺪه اﻧﺪ. ﺑﻪ دﻟﻴﻞ ﻛﻤﺒﻮد اﻃﻼﻋﺎت، ﺗﻨﻮع ﺑﺴﻴﺎر در ﻇﺎﻫﺮ، ﻓﺮا ﺳﺎﺧﺘﺎر و ﺗﺮﻛﻴﺐ اﺟﺴﺎم ﺑﺎﻟﺒﻴﺎﻧﻲ، ﻋﻤﻠﻜﺮد آﻧﻬﺎ در روﻧﺪ ﺗﺨﻤﻚ زاﻳﻲ، ﺗﺮﻛﻴﺐ ﺳﺎﺧﺘﺎري و ﺗﻔﺎوتﻫﺎي ﺑﻴﻦ ﮔﻮﻧﻪاي آن ﺑﻪ درﺳﺘﻲ ﺷﻨﺎﺧﺘﻪ ﻧﺸﺪه اﺳﺖ، و ﺑﺎﺗﻮﺟﻪ ﺑﻪ اﻳﻨﻜﻪ در داﺧﻞ ﻛﺸﻮر ﻣﻄﺎﻟﻌﻪ اي در ﺧﺼﻮص اﺟﺴﺎم ﺑﺎﻟﺒﻴﺎﻧﻲ اﻧﺠﺎم ﻧﺸﺪه اﺳﺖ، ﻫﺪف از ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ، ﺑﺮرﺳﻲ ﻣﻴﻜﺮوﺳﻜﻮپ ﻧﻮري اﺟﺴﺎم ﺑﺎﻟﺒﻴﺎﻧﻲ در 4 ﮔﻮﻧﻪ از ﻣﺎﻫﻴﺎن اﺳﺘﺨﻮاﻧﻲ ﺷﺎﻣﻞ دو ﮔﻮﻧﻪ )ﺷﺎﻧﻚ زرد ﺑﺎﻟﻪ و ﺑﻴﺎح( ﻣﺮﺑﻮط ﺑﻪ آب ﻟﺐ ﺷﻮر و دو ﮔﻮﻧﻪ )ﺑﻨﻲ و ﻛﭙﻮر ﻣﻌﻤﻮﻟﻲ( ﻣﺮﺑﻮط ﺑﻪ آب ﺷﻴﺮﻳﻦ و ﻧﻴﺰ ﺷﺎﻧﻚ زرد ﺑﺎﻟﻪ و ﺑﻴﺎح ﺟﻬﺖ ﺑﺮرﺳﻲ دو ﮔﻮﻧﻪ از دو ﺧﺎﻧﻮاده ﻣﺠﺰا و ﺑﻨﻲ و ﻛﭙﻮر ﻣﻌﻤﻮﻟﻲ ﺟﻬﺖ ﺑﺮرﺳﻲ دو ﮔﻮﻧﻪ از ﻳﻚ ﺧﺎﻧﻮاده ﻣﻲ ﺑﺎﺷﺪ. ﺑﻪ اﻳﻦ ﻣﻨﻈﻮر از ﻫﺮ ﮔﻮﻧﻪ، 5 ﻣﺎﻫﻲ ﻣﺎده ﺑﺎﻟﻎ ﺻﻴﺪ ﺷﺪه و ﺗﺨﻤﺪان ﻫﺎ ﭘﺲ از ﻣﺮاﺣﻞ ﭘﺎﺳﺎژ ﺑﺎﻓﺘﻲ ﻣﻮرد ﺑﺮرﺳﻲ ﻫﻴﺴﺘﻮﻟﻮژﻳﻚ و ﻫﻴﺴﺘﻮﻣﺘﺮﻳﻚ ﻗﺮارﮔﺮﻓﺘﻨﺪ. ﻧﺘﺎﻳﺞ ﻧﺸﺎن داد ﻛﻪ اﺟﺴﺎم ﺑﺎﻟﺒﻴﺎﻧﻲ در ﻓﻮﻟﻴﻜﻮلﻫﺎي ﭘﻴﺶ زرده ﺳﺎزي ﻣﺎﻫﻲ ﺑﻨﻲ، ﻛﭙﻮر ﻣﻌﻤﻮﻟﻲ و ﺷﺎﻧﻚ زرد ﺑﺎﻟﻪ وﺟﻮد دارﻧﺪ. ﻗﻄﺮ اﺟﺴﺎم ﺑﺎﻟﺒﻴﺎﻧﻲ در ﻣﺎﻫﻲ ﺑﻨﻲ ﻛﻪ واﺟﺪ اووﺳﻴﺖ ﻫﺎي ﭘﻴﺶ زرده ﺳﺎزي ﺑﺰرﮔﺘﺮي ﻣﻲ ﺑﺎﺷﺪ ﻧﺴﺒﺖ ﺑﻪ ﻛﭙﻮر ﻣﻌﻤﻮﻟﻲ و ﺷﺎﻧﻚ زرد ﺑﺎﻟﻪ ﺑﻴﺸﺘﺮ ﺑﻮد. ﻧﺘﺎﻳﺞ اﻳﻦ ﺑﺮرﺳﻲ ﻧﺸﺎن داد ﻛﻪ اﺟﺴﺎم ﺑﺎﻟﺒﻴﺎﻧﻲ در اووﺳﻴﺖ ﻫﺎي ﻣﺎﻫﻲ ﺑﻴﺎح ﻣﺸﺎﻫﺪه ﻧﺸﺪ ﻛﻪ ﻣﻲ ﺗﻮاﻧﺪ ﻧﺸﺎن دﻫﻨﺪه ﺗﻔﺎوت ﻫﺎي ﺑﻴﻦ ﮔﻮﻧﻪ اي در روﻧﺪ ﺗﺨﻤﻚ زاﻳﻲ ﺑﺎﺷﺪ.

Balbiani bodies are known as a determinant structures of the fate of germ cells, organells formation and their final location in the oocyte. Due to lack of published information and high diversity in appearance, ultrastructure and composition of balbiani bodies, their function in the process of oogenesis, structional composition and differences between species are not well known, and considering that no study has been done on balbiani bodies in the country, The aim of the present study was light microscopic survey of the balbiani bodies in the oocytes of four species of osteichthyes including two brackish water fish species (Yellowfin seabream and Abu Mullet) and two fresh water fish species (Benni and Common carp), as well as yellowfin seabream and abu mullet to examine two species from two separate families and benni and common carp to examine two species of a family. For this purpose, 5 adult female fish were caught and the ovaries were histologically and histometrically examined after tissue passage. The results showed that balbiani bodies were found in previtellogenesis follicles in benni, common carp and yellowfin seabream. Balbiani body diameter in benni with larger previtellogenesis oocytes, is more than common carp and yellowfin seabream significantly. Results showed that, balbiani body were not exist in abu mullet which can indicate differences between species in the process of oogenesis.