تأثير زمان استخراج بر ويژگي‌هاي فيزيكوشيميايي ژلاتين حاصل از پوست ماهي كاراس (Carassius carassius)

Effect of extraction time on properties of gelatin from the skin of crucian carp (Carassius carassius)

در اين مطالعه، از پوست ماهي كاراس ( Carassius carassius) براي توليد ژلاتين استفاده شد. استحكام ژل ( 6/67) حاصل از ژلاتين استخراج شده طي زمان هاي 1، 3 و 6 ساعت بين 106- 110 گرم بود (0/05P). ساختار ثانويه ژلاتين تحت تأثير زمان استخراج قرار گرفت. نسبت AIII/A1450 در نمونه هاي ژلاتين بين 0/85 الي 0/86 بوده كه نشان دهنده شكستگي مارپيچ سه گانه در كلاژن مي باشد. از بررسي طيف سنجي (FTIR) مشخص گرديد كه ژلاتين استخراج شده در زمان 6 ساعت، به ميزان بيش تري هيدروليز گرديد Δν(νI - νII) (114 cm-1) (114) پوست ماهي كاراس مي تواند به عنوان يك منبع ارزشمند براي توليد ژلاتين مورد استفاده قرار گيرد.

Crucian carp (Carassius carassius) skin was used for production of gelatin. The gel strength of gelatins extracted for 1, 3 and 6 h was between 106-110 g (P>0.05). Glycine (349.21-350.29 residues/ 1000 residues), alanine (131.15-132.73 residues/ 1000 residues), glutamine (81.76-82.57 residues/ 1000 residues) and proline (89.95-96.48 residues/ 1000 residues) were the main amino acids found in gelatins. Extraction time did not change the amino acid composition of gelatin for most of the amino acids; however the content of proline decreased with increasing extraction time. Gelatin extracted for 3 h had different color compared to other gelatins (P<0.05).Secondary structure of gelatin was influenced by extraction time. AIII/A1450 ratio of extracted gelatins was between 0.85-0.86 which confirmed the loss of triple helix in gelatin. Fourier transform infrared (FTIR) spectroscopy indicated that gelatin extracted for 6 h had significantly higher Δν(νI - νII) (114 cm-1) (114) indicating higher denaturation of α-helix chain. Crusian carp skin could serve as a valuable source for production of gelatin.