ارزيابي اثر مهار كنندگي نانوذره اكسيدروي بيوسنتتيك بر بيان ژنهاي افلاكس پمپ (AdeA, AdeC) در سويه هاي باليني Acinetobacter baumannii

Evaluation of the Inhibitory Effect of Biosynthetic Zinc Oxide Nanoparticles on the Expression of Efflux Pumps Genes (AdeA, AdeC) in Clinical Strains of Acinetobacter baumannii

ﻣﻌﺮﻓﯽ ﯾﮏ ﻋﺎﻣﻞ ﺿﺪﺑﺎﮐﺘﺮﯾﺎﯾﯽ ﻣﺆﺛﺮ اﻫﻤﯿﺖ ﻓﺮاواﻧﯽ در ﮐﻨﺘﺮل ﺳـﻮﯾﻪ ﻫـﺎي ﺑﺎﮐﺘﺮﯾـﺎﯾﯽ ﺑﯿﻤـﺎري زا و ﻣﻘﺎوم ﺑﻪ آﻧﺘﯽﺑﯿﻮﺗﯿﮏﻫﺎ دارد. ﭘﯿﺸﺮﻓﺖﻫﺎي ﮔﺬﺷﺘﻪ در زﻣﯿﻨﻪ ﻧﺎﻧﻮﺗﮑﻨﻮﻟﻮژي، ﺑﻪوﯾﮋه ﺗﻮاﻧـﺎ ﯾﯽ ﺳـﺎﺧﺖ ﻧﺎﻧﻮذرات ﺑﯿﻮﻟﻮژﯾﮏ ﻣﻨﺠﺮ ﺑﻪ اﯾﺠﺎد دﺳﺘﻪ وﺳﯿﻌﯽ از ﻋﻮاﻣﻞ ﺿﺪﻣﯿﮑﺮوﺑﯽ ﺷـﺪه اﺳـﺖ ؛ ﻟـﺬا ﻫـﺪف از اﻧﺠﺎم اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﺑﺮرﺳﯽ ﺧﻮاص آﻧﺘﯽﺑﺎﮐﺘﺮﯾﺎﯾﯽ ﻧﺎﻧﻮذره اﮐﺴـﯿﺪ روي )ZnO( در ﻣﻬـﺎر ﺳـﻮﯾﻪ ﻫـﺎي ﻣﻘﺎوم Acinetobacter baumannii ﺑﻪ ﮔﺮوهﻫﺎي ﻣﺨﺘﻠﻒ آﻧﺘﯽﺑﯿـﻮﺗﯿ ﮑﯽ اﺳـﺖ . از ﻣﺠﻤـﻮع 100 ﻧﻤﻮﻧﻪ ﺑﺎﮐﺘﺮﯾﺎﯾﯽ ﻣﺨﺘﻠﻒ، ﺗﻌﺪاد 10 ﻧﻤﻮﻧﻪ آﺳﯿﻨﺘﻮﺑﺎﮐﺘﺮ از ﻣﺮاﮐﺰ ﺑﺎﻟﯿﻨﯽ ﺷﻬﺮﺳـﺘﺎن ﺧـﺮم آﺑـﺎد در ﺑـﺎزه زﻣﺎﻧﯽ ﺷﺶ ﻣﺎﻫﻪ اول ﺳﺎل 1399 ﺟﻤﻊآوري و ﺑﺮ اﺳﺎس آزﻣﻮنﻫﺎي ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ ﺗﻌﯿﯿﻦ ﻫﻮﯾﺖ ﺷـﺪ ﭘﺲ از ﺗﯿﻤﺎر ﺳﻮﯾﻪﻫﺎي MDR ﺑﺎ ﺣﺪاﻗﻞ ﻏﻠﻈﺖ ﻣﻬﺎري ﻧﺎﻧﻮذره اﮐﺴﯿﺪ روي، ﻣﯿـﺰان ﺑﯿـﺎن ژنﻫـﺎي اﻓﻼﮐﺲ ﭘﻤﭗ AdeA و AdeC ﺑﺎ اﺳﺘﻔﺎده از روشReal-time PCR ارزﯾﺎﺑﯽ ﺷﺪ. ﺑﻌﺪ از اﻋﻤﺎل اﺛـﺮ ﻧﺎﻧﻮذره اﮐﺴﯿﺪ روي در ژن AdeA، 80 درﺻﺪ ﻧﻤﻮﻧـﻪ ﻫـﺎ ﮐـﺎﻫﺶ ﺑﯿـﺎن را ﻧﺸـﺎن دادﻧـﺪ. 20 درﺻـﺪ ﻧﻤﻮﻧﻪﻫﺎ ﻧﯿﺰ ﺑﯿﺎن اﻓﺰاﯾﺸﯽ داﺷﺘﻨﺪ. ﻫﻤﭽﻨﯿﻦ در ژن AdeC ، 40 درﺻـﺪ ﻧﻤﻮﻧـﻪ ﻫـﺎ ﺑﻌـﺪ از اﻋﻤـﺎل اﺛـﺮ ﻧﺎﻧﻮذره اﮐﺴﯿﺪ روي ﮐﺎﻫﺶ ﺑﯿﺎن را ﻧﺸﺎن دادﻧﺪ. 60 درﺻﺪ ﻧﻤﻮﻧﻪﻫﺎ ﻧﯿﺰ ﺑﯿﺎن اﻓﺰاﯾﺸـﯽ داﺷـﺘﻨﺪ. ﻃﺒـﻖ ﻧﺘﺎﯾﺞ اﯾﻦ ﭘﮋوﻫﺶ، اﺛﺮ ﻣﻬﺎرﮐﻨﻨﺪﮔﯽ ﻣﻄﻠﻮب ﻧـﺎﻧﻮذره اﮐﺴـ ﯿﺪ روي ﺑﯿﻮﺳـﻨﺘﺘ ﯿﮏ ﺑـﺮ ﺑﯿـﺎن ژنﻫـﺎي AdeA و AdC ﮐﻪ ﻧﻘﺶ ﻣﻬﻤﯽ در ﻣﻘﺎوﻣﺖ داروﯾﯽ ﮔﻮﻧﻪﻫـﺎي آﺳـﯿ ﻨﺘﻮﺑﺎﮐﺘﺮ دارﻧـﺪ ﻣﺸـﻬﻮد ﺑـﻮد . از ﻃﺮﻓﯽ ﺑﺎﺗﻮﺟﻪﺑﻪ ﺑﯿﺎن اﻓﺰاﯾﺸﯽ در 60 درﺻـﺪ ﻧﻤﻮﻧـﻪ ﻫـﺎ ﺑـﺮاي ژن AdeC اﺣﺘﻤـﺎل ﺑـﺮوز ﺟﻬـﺶ در ژنﻫﺎي ﺗﻨﻈﯿﻢﮐﻨﻨﺪه را ﻣﻄﺮح ﺳﺎﺧﺖ. ﺑﻪ ﻧﻈﺮ ﻣﯽرﺳﺪ در آﯾﻨﺪه ﻣـﯽ ﺗـﻮان از ﻧـﺎﻧﻮذره اﮐﺴـ ﯿﺪ روي ﺑﻪﻋﻨﻮان ﯾﮏ ﮐﺎﻧﺪﯾﺪ داروﯾﯽ در داروﺳﺎزي اﺳﺘﻔﺎده ﮐﺮد.

Introducing an effective antibacterial agent is very important in controlling pathogenic and antibiotic-resistant bacterial strains. Recent advances in nanotechnology, especially the ability to fabricate biological nanoparticles, have led to the development of a wide range of antimicrobial agents. Therefore, the aim of this study is to investigate the antibacterial properties of ZnO nanoparticles (NPs) in inhibiting resistant strains of Acinetobacter baumannii. Ten samples of Acinetobacter were collected from clinical centers of Khorramabad city in 2020 and were identified based on biochemical tests. After treatment of MDR strains with sub-minimum inhibitory concentration (MIC) of ZnO NPs, the expression level of efflux pump genes (AdeA, AdeC) was evaluated using real-time PCR. After applying the effect of ZnO NPs on AdeA gene, 80% of the samples showed reduced expression. 20% of the sample also had incremental expression. Also in AdeC gene, 40% of the samples showed reduced expression after the effect of ZnO NPs. 60% of the samples also had incremental expression. According to the results, the optimal inhibitory effect of biosynthesized ZnO NPs on the expression of AdeA and AdC genes, which play an important role in drug resistance of the Acinetobacter species, was evident. On the other hand, due to the increased expression in 60% of the samples for the AdeC gene, the possibility of mutations in the regulatory genes was raised. It seems that ZnO NPs can be used as a pharmaceutical candidate in the pharmaceutical industry in the future