ﺗﺄﺛﯿﺮ ﺣﻠّﺎل ﻫﺎي ﻣﺨﺘﻠﻒ ﺑﺮ ﻣﯿﺰان درﺻﺪ ﻣﻬﺎر رادﯾﮑﺎل آزاد DPPH و ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽ -ا ﮐﺴﯿﺪاﻧﯽ ﮔﯿﺎﻫﺎن داروﯾﯽ ﺑﻮﻣﯽ ﺷﻬﺮﺳﺘﺎن ﺟﯿﺮﻓﺖ

The Effect of Different Solvents on the Percentage of Free Radical Scavenging DPPH and Antioxidant Activity of Native Medicinal Plants in Jiroft City

در ﻗﺮن ﺣﺎﺿﺮ، ﺗﺤﻘﯿﻘﺎت ﮔﺴﺘﺮده اي در ﻣﻮرد ﮔﯿﺎﻫﺎن داروﯾﯽ اﻧﺠﺎم ﺷﺪه و داروﻫﺎﯾﯽ ﺑﺎ ﻣﻨﺸﺄ ﻃﺒﯿﻌﯽ اﻓﻖﻫﺎي ﺟﺪﯾﺪي را ﺑﺮاي ﺟﺎﻣﻌﻪ ﭘﺰﺷﮑﺎن، داروﺳﺎزان و ﭘﮋوﻫﺸﮕﺮان ﮔﺸﻮده اﺳﺖ. ﻫﺪف از اﯾﻦ ﻣﻄﺎﻟﻌﻪ، ﺑﺮرﺳﯽ ﺗﺄﺛ ﯿﺮ ﺣﻼلﻫﺎي ﻣﺨﺘﻠﻒ ﺑﺮ ﻣﯿﺰان درﺻﺪ ﻣﻬﺎر رادﯾﮑﺎل آزاد DPPHو ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ ﮔﯿﺎﻫﺎن داروﯾﯽ ﺑﻮﻣﯽ ﺷﻬﺮﺳﺘﺎن ﺟﯿﺮﻓﺖ ﻣﯽﺑﺎﺷﺪ. در اﯾﻦ ﭘﮋوﻫﺶ، ﺷﺶ ﮔﯿﺎه داروﯾﯽ ﺑﻮﻣﯽ ﺷﻬﺮﺳﺘﺎن ﺟﯿﺮﻓﺖ )آوﯾﺸﻦ، ﻣﺮزه، ﻧﻌﻨﺎع ﻓﻠﻔﻠﯽ، ﮐﺎﮐﻮﺗﯽ ﮐﻮﻫﯽ، رزﻣﺎري و ﺑﻮﻣﺎدران( آزﻣﺎﯾﺶ ﺷﺪﻧﺪ. ﻋﺼﺎره ﮔﯿﺮي از ﻗﺴﻤﺖﻫﺎي ﻫﻮاﯾﯽ ﮔﯿﺎﻫﺎن ﺑﺎ اﺳﺘﻔﺎده از ﺣﻼلﻫﺎي ﻣﺘﺎﻧﻮل 80درﺻﺪ، اﺗﺎﻧﻮل 50 درﺻﺪ، اﺳﺘﻮن و ﻫﮕﺰان ) 100درﺻﺪ( اﻧﺠﺎم ﺷﺪ. ﻣﯿﺰان ﻓﻌﺎﻟﯿﺖ آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ ﺑﻪ روش 2و 2دي ﻓﻨﯿﻞ-1 - ﭘﯿﮑﺮﯾﻞ ﻫﯿﺪرازﯾﻞ، درﺻﺪ ﻣﻬﺎر رادﯾﮑﺎل آزاد DPPHاﻧﺪازه ﮔﯿﺮي ﺷﺪ. از ﺑﯿﻦ ﺣﻼلﻫﺎي ﻣﻮردﻣﻄﺎﻟﻌﻪ در ﻫﺮ ﺷﺶ ﮔﯿﺎه ،ﻋﺼﺎره ﻫﺎي اﺳﺘﺨﺮاج ﺷﺪه ﺑﺎ ﺣﻼل ﻣﺘﺎﻧﻮل 80 درﺻﺪ، ﺑﯿﺸﺘﺮﯾﻦ و ﺣﻼل ﻫﮕﺰان ﮐﻤﺘﺮﯾﻦ ﻣﯿﺰان ﺟﺬب رادﯾﮑﺎل آزاد ﺑﻮد. از ﺑﯿﻦ ﮔﯿﺎﻫﺎن ﻣﻮردﻣﻄﺎﻟﻌﻪ، ﻧﻌﻨﺎع ﻓﻠﻔﻠﯽ در اﺳﺘﺨﺮاج ﺑﺎ ﻫﺮ ﭼﻬﺎر ﺣﻼل )ﺣﻼل ﻣﺘﺎﻧﻮل IC50:20.9 ppm، اﺗﺎﻧﻮل IC50:34ppm، اﺳﺘﻮنIC50:44.2ppm و ﻫﮕﺰان IC50:49.19ppm( داراي ﮐﻤﺘﺮﯾﻦ IC50و ﮔﯿﺎه ﮐﺎﮐﻮﺗﯽ ﮐﻮﻫﯽ )ﺣﻼل ﻣﺘﺎﻧﻮل IC50:1090.78ppm، اﺗﺎﻧﻮل IC50:1209.3ppm، اﺳﺘﻮن IC50:1439.23ppmو ﻫﮕﺰان IC50:1898.7ppm( داراي ﺑﺎﻻﺗﺮﯾﻦ ﻣﻘﺪار ﺑﻮد. ﻫﻤﭽﻨﯿﻦ ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﮐﻪ ﺗﻤﺎﻣﯽ ﺷﺶ ﮔﯿﺎه داروﯾﯽ ﻣﻮرد ﭘﮋوﻫﺶ، ﺧﻮاص ﺿﺪرادﯾﮑﺎﻟﯽ دارﻧﺪ و ﻋﺼﺎره اﻟﮑﻠﯽ ﻣﺘﺎﻧﻮﻟﯽ 80درﺻﺪ اﯾﻦ ﮔﯿﺎﻫﺎن ﻣﯽﺗﻮاﻧﻨﺪ در ﺻﻨﺎﯾﻊ ﻣﺨﺘﻠﻒ ﺑﻪ ﻋﻨﻮان ﻣﻮاد آﻧﺘﯽاﮐﺴﯿﺪان ﻃﺒﯿﻌﯽ اﺳﺘﻔﺎده ﺷﻮﻧﺪ.

In the current century, extensive research has been carried out on medicinal plants, and drugs of natural origin have opened new horizons for the community of doctors, pharmacists and researchers. The aim of this study was to investigate the effect of different solvents on the percentage of free radical scavenging DPPH and antioxidant activity of native medicinal plants in Jiroft city and six native plants (Achillea millefoliom, Thymus vulgaris, Satureja hortensis, Ziziphora Clinopodioides, Mentha piperita and Rosmarinus officinalis) were sampled for testing. Extraction of the aerial parts of plants was performed using 80% methanol, 50% ethanol, acetone 100% and hexane 100% solvents. The extraction time was 24 hours at ambient temperature using a stirrer. The amount of antioxidant activity was measured using 2 and 2 diphenyl-1-pyrrolidrazyl, DPPH free radical inhibitory concentration. Among the studied solvents in all six plants, the extracts extracted with 80% methanol solvent had the highest and the hexane solvent had the lowest free radical adsorption. Among the studied plants, peppermint extract with all four solvents (methanol IC50: 20.9 ppm, ethanol IC50: 34ppm, acetone IC50: 44.2ppm, and hexane IC50: 49.19ppm) had the highest antiradical activity and Ziziphora Clinopodioides (methanol IC50: 1090.78ppm, ethanol IC50: 1209.3ppm, acetone IC50: 1439.23ppm and hexane IC50: 1898.7ppm) had the lowest anti-radical activity. The findings of the present study showed that all the 6 studied medicinal plants had anti-radical properties and using 80% methanol extracts of these plants can be used as an antioxidant in various industries.